Genetically encoded conductive protein nanofibers secreted by engineered cells

Kalyoncu, Ebuzer; Ahan, Recep Erdem; Ölmez, Tolga T.; Şeker, Urartu Özgür Şafak

doi:10.1039/c7ra06289c

Cited by 40 publications

(43 citation statements)

References 72 publications

(31 reference statements)

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“…In fact, various types of synthetic conductive protein fibres have recently been engineered using recombinant DNA technologies and engineered microbes as chassis. [19][20][21][22] These novel conductive biomaterials hold Sophia Roy and Oliver Xie contributed equally.…”

Section: Introductionmentioning

confidence: 99%

Challenges in engineering conductive protein fibres: Disentangling the knowledge

2020

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Conductive protein materials are promising candidates for next‐generation bioelectronics due to their genetically‐customizable functionalities, biocompatibility, and bioactivity. We envision that they could be used in a variety of bio‐friendly functional devices, including bio‐electronic interfaces, bio‐energy devices, and sensors. However, their practical uses are limited by gaps in our understanding of charge transport in proteins, and by challenges in establishing reliable data collection methods. Moreover, characterization protocols are not always designed with applications in mind, which hinders engineering developments. Here, we review the effects of sample preparation, environmental conditions (ie, hydration level, pH, temperature), measurement scale (nano, micro, and macro), and geometrical considerations, on the measured electrical properties of proteins. We emphasize the need for standardized methods and collaborations across fields for the design of conductive protein materials, keeping in mind their end goal applications. Our objective for this review is to disentangle the knowledge on protein conductivity, and to clarify the current challenges, limitations, and future possibilities for these biological conductors.

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Section: Introductionmentioning

confidence: 99%

Challenges in engineering conductive protein fibres: Disentangling the knowledge

2020

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“…Curli have already been engineered to develop strong underwater adhesives, 9 multifunctional biolms, 10 template nanoparticles 10,32 and quantum dots, 32 and more. 33,34 However, the complete atomistic structure of neither CsgA nor CsgB has been experimentally determined. As the structure of a protein dictates its' function, knowledge of specic protein structure is of great importance in fully understanding curli formation and function, and utilizing these structures in bioengineering applications.…”

Section: Introductionmentioning

confidence: 99%

Structural predictions for curli amyloid fibril subunits CsgA and CsgB

DeBenedictis

Keten

2017

RSC Adv.

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“…This was first accomplished by displaying conductive gold nanoparticle binding domains on CsgA in order to create a bio‐inorganic hybrid material (Figure f) . Another example involved fusing an aromatic residue‐rich domain derived from the PilA protein of the electroactive marine bacterium, Geobacter sulfurreducens to achieve conductive fibers that were completely genetically encoded …”

Section: Engineering Cells and Biofilms As Living Materialsmentioning

confidence: 99%

Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials

et al. 2018

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Vast potential exists for the development of novel, engineered platforms that manipulate biology for the production of programmed advanced materials. Such systems would possess the autonomous, adaptive, and self-healing characteristics of living organisms, but would be engineered with the goal of assembling bulk materials with designer physicochemical or mechanical properties, across multiple length scales. Early efforts toward such engineered living materials (ELMs) are reviewed here, with an emphasis on engineered bacterial systems, living composite materials which integrate inorganic components, successful examples of large-scale implementation, and production methods. In addition, a conceptual exploration of the fundamental criteria of ELM technology and its future challenges is presented. Cradled within the rich intersection of synthetic biology and self-assembling materials, the development of ELM technologies allows the power of biology to be leveraged to grow complex structures and objects using a palette of bio-nanomaterials.

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Genetically encoded conductive protein nanofibers secreted by engineered cells

Abstract: Bacterial biofilms are promising tools for functional applications as bionanomaterials.

Cited by 40 publications

References 72 publications

Challenges in engineering conductive protein fibres: Disentangling the knowledge

Challenges in engineering conductive protein fibres: Disentangling the knowledge

Structural predictions for curli amyloid fibril subunits CsgA and CsgB

Engineered Living Materials: Prospects and Challenges for Using Biological Systems to Direct the Assembly of Smart Materials

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