Genetically different cell subpopulations in hydatidiform moles A study of three cases by RFLP, flow cytometric, cytogenetic, HLA, and morphologic analyses

Sunde, Lone; Vejerslev, Lars O.; Larsen, Jørgen K.; Christensen, Ib Jarle; Hansen, Hanna E.; Mogensen, Bent; Bolund, Lars

doi:10.1016/0165-4608(89)90047-2

Cited by 11 publications

(9 citation statements)

References 26 publications

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“…To confirm this hypothesis, further investigations are necessary by direct and culture chromosome preparations as well as by flow cytometry. These results, as well as those of reported cases where the maternal and paternal chromosomal sets were balanced [14], chromosomal set was not triploid [16], or where there were heterogenic cell populations [ 15] make it prob able, that not just the abnormal fertilization resulting in an extra paternal chromosomal set can cause the degener ations characteristic for partial hydatidiform moles.…”

Section: Discussionsupporting

confidence: 69%

“…The cells cultured for chromosome analysis were of mesenchy mal origin, whereas in vivo mitoses occur in trophoblastic cells. A few cases of genetically different cell subpopula tions in hydatidiform moles have been reported [ 15], but this is difficult to disclose by cytogenetic analysis of only cultured cells. The culture conditions may favour the growth of one-cell subpopulations, which will therefore dominate at the time of harvest.…”

Section: Discussionmentioning

confidence: 99%

“…Another possibility is that the abnormal cell lines have a selective disadvantage. It was demonstrated by RFLP and flow cytometric analy sis, that genetically different cell subpopulations may exist in hydatidiform moles though cytogenetic investiga tions have not shown mosaicism [15].…”

Section: Discussionmentioning

confidence: 99%

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Tetraploidy in Human Placenta

Tóth¹,

Arató²,

Szepesi³

et al. 1992

Gynecol Obstet Invest

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17 cases of partial molar pregnancy were analysed cytogenetically by the direct-preparation method. Eight partial moles were triploid, 7 diploid/tetraploid mosaic, and 2 tetraploid. In the course of prenatal cytogenetic screening, out of 1,263 chorionic villus samplings, 2 tetraploid and 1 diploid/tetraploid cases were found. These cases of partial moles do not fit into the usual patterns of triploid partial moles. The findings presented here suggest that different causative factors may be involved in the origin of molar degenerations. These results also call to attention that tetraploidy is an existent and relatively common abnormality.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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Tetraploidy in Human Placenta

Tóth¹,

Arató²,

Szepesi³

et al. 1992

Gynecol Obstet Invest

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“…The approved doses of TNFi for treatment of AS are 5 mg/kg infliximab intravenously every 6–8 weeks after induction; subcutaneous etanercept, 25 mg twice a week or 50 mg once a week; 50 mg subcutaneous golimumab monthly and 40 mg adalimumab subcutaneously every other week (category A/B evidence23 281–288 322 398 399). No major differences in efficacy and safety between 50 mg and 100 mg golimumab doses were seen when treating AS (category B evidence272).…”

Section: Tnf Blocking Agents (Tnfi)mentioning

confidence: 99%

“…In clinical trials in patients fulfilling the modified New York criteria for AS, improvement in signs and symptoms were seen after TNFi as measured by patient-reported outcomes (BASDAI, BASFI, patient global VAS, SF-36), spinal mobility measures, peripheral arthritis, enthesitis and acute phase reactants (category A/B/D evidence257 280–288 395 396 398 400–402). …”

Section: Tnf Blocking Agents (Tnfi)mentioning

confidence: 99%

Updated consensus statement on biological agents for the treatment of rheumatic diseases, 2012: Table 1

Fürst

Keystone

So³

et al. 2013

Ann Rheum Dis

119

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Interphase cytogenetics in paraffin sections of routinely processed hydatidiform moles and hydropic abortions

Kaa

Nelson

Ramaekers

et al. 1991

The Journal of Pathology

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The differential diagnosis of complete (CM) and partial (PM) hydatidiform moles and hydropic abortions (HA) can be difficult when based on histology alone. Therefore, a more objective approach of chromosome ploidy analysis as detected by in situ hybridization (ISH) was performed on 6 microns paraffin sections of seven cases, originally classified as three CM, two PM, and two HA with a histologic pattern suggestive of triploidy. Probes for repetitive DNA targets in the (peri)centromeric region of chromosomes 1 and X and in the q arm of chromosome Y were used to determine chromosome ploidy and sex chromosome composition. The findings in the three CM were consistent with diploidy: two copies of chromosomes 1 and X and none of chromosome Y. In the two HA with a histologic pattern suggestive of triploidy, three copies of chromosomes 1 and X and none of chromosome Y confirmed triploidy. Two cases originally classified as PM both appeared to have two copies of chromosome 1 with an XX pattern in one case and an XY pattern in the other case, which is consistent with diploidy instead of triploidy. After reviewing, both cases most likely represented CM. We conclude that interphase cytogenetics by ISH on paraffin sections of hydatidiform moles and hydropic abortions enables chromosome ploidy analysis with preservation of histological context.(ABSTRACT TRUNCATED AT 250 WORDS)

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Genetically different cell subpopulations in hydatidiform moles A study of three cases by RFLP, flow cytometric, cytogenetic, HLA, and morphologic analyses

Cited by 11 publications

References 26 publications

Tetraploidy in Human Placenta

Tetraploidy in Human Placenta

Updated consensus statement on biological agents for the treatment of rheumatic diseases, 2012: Table 1

Interphase cytogenetics in paraffin sections of routinely processed hydatidiform moles and hydropic abortions

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