Genetical dissection of H3-mediated polygenic PCN resistance in a heterozygous autotetraploid potato population

Bryan, Glenn J.; McLean, Karen; Pande, B. N.; Purvis, Andy; Hackett, Christine A.; Bradshaw, J. E.; Waugh, Robbie

doi:10.1023/b:molb.0000037999.13581.9c

Cited by 45 publications

(32 citation statements)

References 21 publications

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“…The percentage of the total phenotypic variation explained by the major QTL on chromosome IX using interval mapping (43%) exceeded the original 27% obtained by simple regression analysis (Tables 2 and 3). A similar increase in the percent of variability explained by an interval mapping approach has been previously observed (Bradshaw et al 2004a, b;Bryan et al 2004). The variation explained by the minor QTL associated with the marker GGT-CAC-0259 was 12.3% in simple regression analysis.…”

Section: Discussionsupporting

confidence: 84%

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Interval Mapping of Quantitative Trait Loci for Corky Ringspot Disease Resistance in a Tetraploid Population of Potato (Solanum tuberosum subsp. tuberosum)

et al. 2008

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Corky ringspot disease (CRS) resistance is an ideal target trait for breeding using marker-assisted selection (MAS) due to the high variability in field disease incidence that complicates screening for resistance. To develop molecular marker(s) associated with CRS resistance, a linkage map was constructed for a tetraploid population of 92 genotypes in order to conduct quantitative trait locus (QTL) analysis. The population was tested for CRS resistance in an infested screening field for four years. Broad sense heritability and its standard error of CRS disease resistance were 0.80 (±0.16). A total of 892 AFLP, 95 SSR, and 5 SSCP markers were scored and used for testing marker-trait association. One major QTL that explained 43% of the phenotypic variation in CRS resistance was localized on chromosome IX, with flanking AFLP markers AAC-CGT-0347 and ACG-CTG-0588. A minor QTL that explained 12% of CRS resistance was also detected. This minor QTL was associated with distorted marker GGT-CAC-0259, which remained unlinked. Polygenetic nature of CRS resistance was explained by major and minor QTLs association. Conversion of the three AFLP markers associated with this quantitative trait to simple PCR markers will benefit resistance breeding by enabling MAS. ResumenLa resistencia a la mancha de anillo corchoso (CRS) es un carácter ideal para el mejoramiento mediante la selección asistida por marcador (MAS), debido a la gran variabilidad de la incidencia de la enfermedad en el campo, la misma que complica el tamizado para resistencia. Para desarrollar marcadores moleculares asociados con resistencia a CRS, se construyó un mapa de ligamiento para una población tetraploide de 92 genotipos, con el fin de realizar un análisis de locus de un carácter cuantitativo (QTL). La población fue probada para resistencia a CRS tamizando por cuatro años en un campo infestado. La heredabilidad en sentido amplio de la resistencia a CRS, y su error estándar, fue de 0.80 (±16). Un total de 892 marcadores AFLP, 95 SSR y 5 SSCP fueron tomados en cuenta y usados para probar la asociación marcador-carácter. Un QTL mayor que explicó el 43% de variación fenotípica en resistencia al CRS fue localizado en el cromosoma IX, con marcadores AFLP flanqueantes, AAC-CGT-0347 y ACG-CTG-0588. Un QTL menor que explicó el 12% de resistencia al CRS fue detectado también. Este QTL menor fue asociado con el marcador distorsionado CGT-CAC-0259, el cual permaneció sin ligamiento. La naturaleza poligenética de la resistencia al CRS fue explicado por la asociación de los QTLs mayor y menor. La conversión de los tres marcadores AFLP asociados con este carácter cuantitativo en marcadores simples PCR, beneficiará el mejoramiento para resistencia haciendo posible la aplicación de MAS.

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Section: Discussionsupporting

confidence: 84%

“…A detailed description of the theory for QTL interval mapping in an autotetraploid species is given by Hackett et al (2001); the protocol for interval mapping was summarized by Bryan et al (2004). The "TetraploidMap" software program (Hackett and Luo 2003) was used for all analytical procedures for the QTL interval mapping.…”

Section: Interval Mapping Of Qtlsmentioning

confidence: 99%

Interval Mapping of Quantitative Trait Loci for Corky Ringspot Disease Resistance in a Tetraploid Population of Potato (Solanum tuberosum subsp. tuberosum)

et al. 2008

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“…For example, Bradshaw et al (2004), Bryan et al (2004), andCaromel et al (2005) could identify linkage groups by the presence of comigrating AFLPs shared with this diploid reference map. Recently, the mapping of the R10 and R11 genes for resistance to late blight (P. infestans) by Bradshaw et al (2006) was entirely based on the mobility-based nomenclature of AFLP markers from this study (http://potatodbase.dpw.wau.nl/UHDdata.…”

Section: Discussionmentioning

confidence: 99%

Construction of a 10,000-Marker Ultradense Genetic Recombination Map of Potato: Providing a Framework for Accelerated Gene Isolation and a Genomewide Physical Map

et al. 2006

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An ultradense genetic linkage map with .10,000 AFLP loci was constructed from a heterozygous diploid potato population. To our knowledge, this is the densest meiotic recombination map ever constructed. A fast marker-ordering algorithm was used, based on the minimization of the total number of recombination events within a given marker order in combination with genotyping error-detection software. This resulted in ''skeleton bin maps,'' which can be viewed as the most parsimonious marker order. The unit of distance is not expressed in centimorgans but in ''bins.'' A bin is a position on the genetic map with a unique segregation pattern that is separated from adjacent bins by a single recombination event. Putative centromeres were identified by a strong clustering of markers, probably due to cold spots for recombination. Conversely, recombination hot spots resulted in large intervals of up to 15 cM without markers. The current level of marker saturation suggests that marker density is proportional to physical distance and independent of recombination frequency. Most chromatids (92%) recombined once or never, suggesting strong chiasma interference. Absolute chiasma interference within a chromosome arm could not be demonstrated. Two examples of contig construction and map-based cloning have demonstrated that the marker spacing was in accordance with the expected physical distance: approximately one marker per BAC length. Currently, the markers are used for genetic anchoring of a physical map of potato to deliver a sequence-ready minimal tiling path of BAC contigs of specific chromosomal regions for the potato genome sequencing consortium (http:/ /www.potatogenome.net).

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“…With a limited number of targeted markers, Bormann et al (2004) tagged QTLs for maturity corrected late blight resistance in tetraploid potato. However, the QTL studies in the tetraploid mapping population 12601ab1 £ Stirling are unique in demonstrating the complexity of map construction and QTL analysis Bryan et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Association mapping of quality traits in potato (Solanum tuberosum L.)

D’hoop¹,

Paulo²,

Mank

et al. 2007

Euphytica

102

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In this paper, we describe the assessment of linkage disequilibrium and its decay in a collection of potato cultivars. In addition, we report on a simple regression based association mapping approach and its results to quality traits in potato. We selected 221 tetraploid potato cultivars and progenitor lines, representing the global diversity in potato, with emphasis on genetic variation for agro-morphological and quality traits. Phenotypic data for these agro-morphological and quality traits were obtained from recent trials performed by Wve breeding companies. The collection was genotyped with 250 AFLP ® markers from Wve primer combinations. The genetic position of a subset of the markers could be inferred from an ultra dense potato map. Decay of linkage disequilibrium was estimated by calculating the squared correlation between pairs of markers using marker band intensities. Marker-trait associations were investigated by Wtting single marker regression models for phenotypic traits on marker band intensities with and without correction for population structure. The paper illustrates the potential of association mapping in tetraploid potato, because existing phenotypic data, a modest number of AFLP markers, and a relatively simple statistical analysis, allowed identifying interesting associations.

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Genetical dissection of H3-mediated polygenic PCN resistance in a heterozygous autotetraploid potato population

Cited by 45 publications

References 21 publications

Interval Mapping of Quantitative Trait Loci for Corky Ringspot Disease Resistance in a Tetraploid Population of Potato (Solanum tuberosum subsp. tuberosum)

Interval Mapping of Quantitative Trait Loci for Corky Ringspot Disease Resistance in a Tetraploid Population of Potato (Solanum tuberosum subsp. tuberosum)

Construction of a 10,000-Marker Ultradense Genetic Recombination Map of Potato: Providing a Framework for Accelerated Gene Isolation and a Genomewide Physical Map

Association mapping of quality traits in potato (Solanum tuberosum L.)

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