Genetical analysis of methionine suppressors in <i>Coprinus</i>

Lewis, D.

doi:10.1017/s0016672300000628

Cited by 100 publications

(74 citation statements)

References 8 publications

(6 reference statements)

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“…The possibility that differences in phenotype may occur between combinations of the same genes in heterokaryons and in heterozygotes was predicted by Pontecorvo (19523) and has been discussed more recently by other authors (Roper, 1958; Lewis, 1961). Differences have been described in Ustilago in the pathogenicity and saprophytic growth of dikaryons as compared with diploids (Holliday, 1961) and also in the case of pun2 mutants of Neurospora combined in heterokaryons or in disomic 'pseudowild' strains (Case & Giles, 1960).…”

Section: Discussionmentioning

confidence: 96%

The Genetic Analysis of Carbohydrate Utilization in Aspergillus nidulans

Roberts¹

1963

Journal of General Microbiology

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SUMMARYThe isolation and genetic analysis of a new class of mutants of the mould Aspergillus nidulans is described. The mutants were detected by their inability to utilize specific carbohydrates as sole carbon source for growth. All of the mutants are recessive and analysis of 27 mutants has resulted in the description of 10 new loci concerned with the utilization of carbohydrates in the organism; the loci have been allocated to linkage groups and 6 were mapped meiotically. Two loci which control lactose utilization are not linked and neither are 5 loci which control the utilization of galactose. A series of sorbitol mutants is unusual in that they are all non-complementary when combined in heterokaryons, although certain pairs of mutants complement in the corresponding heterozygous diploids.

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Section: Discussionmentioning

confidence: 96%

The Genetic Analysis of Carbohydrate Utilization in Aspergillus nidulans

Roberts¹

1963

Journal of General Microbiology

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“…C. cinerea strains were maintained at 37ºC on complete medium (CM) (Lewis, 1961) supplemented with 100 μg/L L-tryptophan or 2 μg/L para-aminobenzoic acid as required. CM broth was used to grow isolates for four days at 37ºC for RNA extraction, fluorimetry and microscopy.…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes

Costa

Mills

Bailey

et al. 2008

Journal of Microbiological Methods

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This technological advance should expedite functional genomic studies in C. cinerea and has wider potential for utility in other homobasidiomycete and filamentous fungi. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

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“…For (GPCRs). Fungal GPCRs constitute the class D subfamgeneral culture and mating tests the complete medium of ily, and C. cinereus GPCRs specifically belong to the S. Lewis (1961) was used, supplemented with 100 mg/liter cerevisiae Ste3p-like receptor subgroup (http:/ /www.gpcr.…”

Section: T He Role Of Pheromone Signaling In Fungal Matingmentioning

confidence: 99%

The Origin of Multiple B Mating Specificities in Coprinus cinereus

et al. 2005

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Mushrooms, such as Coprinus cinereus, possess large families of pheromones and G-protein-coupled receptors that are sequestered at the B mating-type locus and whose function is to confer vast numbers of different mating types. This ability results from complex patterns of cognate and noncognate pheromone/ receptor pairings, which potentially offer a unique insight into the molecular interaction between receptor and ligand. In this study we have identified many more members of these families by molecular analysis of strains collected worldwide. There are three groups of genes at each B locus. We have identified two alleles of group 1, five alleles of group 2, and seven alleles of group 3, encoding in total 14 different receptors and 29 different pheromones. The specificity of many newly identified alleles was determined by transformation analysis. One striking finding was that receptors fall into groups based on sequence homology but these do not correspond to the groups defined by position, indicating that complex evolutionary processes gave rise to the B loci. While additional allelic versions may occur in nature, the number of B specificities possible by combination of the alleles that we describe is 70, close to previous estimates based on population analysis. T HE role of pheromone signaling in fungal matingand receptors were first identified as mating-type deterhas largely been elucidated from studies of the budminants and map to what has been designated the B ding yeast Saccharomyces cerevisiae. This fungus has just mating-type locus. A second mating-type locus, A, entwo mating types and pheromones are secreted to act codes the subunits of a transcription factor belonging as chemoattractants for identifying compatible mating to the homeodomain family (Kü es et al. 1994a) that is partners. Binding of a pheromone to an appropriate necessary, together with pheromone signaling, to proreceptor on the cell surface triggers an intracellular mote the initial stages of sexual development. Compati-G-protein-linked MAP kinase cascade that results in ble A genes encode versions of the proteins that can changes in growth direction to permit cell fusion, which heterodimerize following cell fusion, an interaction that is then followed immediately by nuclear fusion (reis analogous to that between the MATa1 and MAT␣2 viewed by Kurjan 1993). Once cells are diploid, pheromating-type proteins in mated cells of S. cerevisiae (remone signaling ceases.viewed by Johnson 1995). Pheromone signaling also plays an essential role in In contrast to S. cerevisiae, compatible cell fusion in mating in basidiomycete fungi (reviewed by Casselton C. cinereus is not followed immediately by nuclear fusion, and Olesnicky 1998), but in mushroom species such as but by an extended vegetative phase in which the nuclei Coprinus cinereus, unlike S. cerevisiae, there is no evidence from each mate remain paired in each cell, a phase that pheromones have a role in mate attraction (Olesknown as the dikaryophase or dikaryon. The B matingnicky et ...

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Genetical analysis of methionine suppressors in Coprinus

Cited by 100 publications

References 8 publications

The Genetic Analysis of Carbohydrate Utilization in Aspergillus nidulans

The Genetic Analysis of Carbohydrate Utilization in Aspergillus nidulans

Oligonucleotide sequences forming short self-complimentary hairpins can expedite the down-regulation of Coprinopsis cinerea genes

The Origin of Multiple B Mating Specificities in Coprinus cinereus

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