Genetic Variations of Outer Membrane Protein Genes of Vibrio harveyi Isolated in Korea and Immunogenicity of OmpW in Olive Flounder, Paralichthys olivaceus

Abstract

Vibrio harveyi is a pathogenic marine bacterium causing systemic symptoms resulting in mass mortalities in fishes and shrimps in aquaculture. Outer membrane proteins(OMPs) are related to the pathogenicity and thus good targets for diagnosis and vaccination for Gram negative bacteria. Recently vaccination strategies using the OMPs have been suggested to control vibriosis in several fish species. In this study, we have isolated V. harveyi from diseased marine fishes from different regions of Korea and investigated genetic variations of four OMP genes including OmpK, OmpU, OmpV and OmpW. Consequently, OmpK and U genes could be divided into 3 subgroups of type I, II, III and type A, B, C, respectively, without any correlation with geographical regions and species while OmpV and W were highly homologous. OmpW gene of V. harveyi FP4138 was fully sequenced and predicted the deduced amino acid sequence to form -barrel with hydrophobic channel. Indeed, the immunogenicity of recombinant OmpW produced in β Escherichia coli was assessed by vaccinating flounder. As a result, the high antibody response with antibody titer of 4.2±0.7 and protection with relative percent survival of 60% against artificial infection of V. harveyi were demonstrated. This result indicates that OmpW is a virulence related factor and it can be a vaccine candidate to prevent a high mortality caused by V. harveyi infection in olive flounder, Paralichthys olivaceus.

Bacteriostatic effects of benzyl isothiocyanate on Vibrio parahaemolyticus: Transcriptomic analysis and morphological verification

“…By BITC treatment, gene expression level of VP2362 was signi cantly downregulated compared with that of control group. The outer membrane protein is virulence related (Kim MS 2015) and commonly found in Gram-negative bacteria (Xiong XP 2010). Previous ndings have con rmed that the cell surface integrity, neutralizing host defense mechanisms, cell adhesion and invasion, and inhibition of the complement system are all affected by outer membrane proteins (Koebnik R 2000).…”

Transcriptomic Analysis of Vibrio parahaemolyticus Reveals Different Virulence Gene Expression in Response to Benzyl Isothiocyanate

“…VPA0318 encodes the outer membrane protein OmpV. Outer membrane proteins (OMPs) are widespread in Gram-negative bacteria [ 36 ] and are related to pathogenicity [ 37 ]. A previous study reported that outer membrane proteins could medicate the infection process including chemotaxis, motility through the mucous layer lining the intestinal wall, and survival of the gastric acid barrier [ 38 ].…”