Genetic Variations in Asparagus racemosus, an Endangered Medicinal Herb Endemic to India Using RAPD Markers

Assessment of genetic diversity of Asparagus racemosus willd. from different agro-ecological zones of Kerala using RAPD markers

“…The presence of large genetic variations within the accessions was clear from the range of Jaccard's similarity coefficient (0.118 to 0.566). Similar to the results the study of KUMAR et al (2016) in A. racemosus accessions revealed the range of Jaccard's coefficient from 0.48 to 0.97. Interestingly in our study the most similar accessions Ar11 and Ar12 are from two different agro-ecological zones viz., zone 7 and 10.…”

“…In the present work primer OPI19 which showed highest resolving power (37.1) was found to be most efficient with respect to A. racemosus. Comparing to this, genetic diversity analysis in 60 A. racemosus cultivars through RAPD revealed RP ranged from 0.8 to 25.17 (KUMAR et al, 2016). The highest Marker Index observed for the primer OPI 12 was not evaluated as a genotype diagnosis tool of a primer but provides information about overall utility of a marker.…”

“…However, only 54.92% of polymorphism was resulted in a study by VIJAY et al (2009) in A. racemosus. In a previous study, KUMAR et al (2016) reported 81.42% of polymorphism in 60 A. racemosus cultivars. LAL et al (2011) had reported 94.50% of polymorphism between 5 different species of Asparagus.…”

“…The efficiency of RAPD primers for the better resolving of polymorphism in the intra-species level is evident from the broad range of PIC values (0.059-0.209). Previous report (KUMAR et al, 2016) suggests the range of PIC from 0.497 to 0.945 in A. racemosus, where as in Aloe barbadensis PIC value was between 0.13 to 0.44 (PANWAR et al, 2013). From the present study it is clear that both TNP and RP are inversely related to PIC.…”

“…There have been extensive studies carried out on genetic diversity in different Asparagus species like A. racemosus, A. srpingeri, A. officinalis, A. plumosus and A. densiflors myersii (LAL et al, 2011) especially A. officinalis using RAPD markers (RAIMONDI et al, 2001;LI et al, 2012;IRSHAD et al, 2014). However, there are scares of literature on genetic diversity studies in A. racemosus (VIJAY et al, 2009;SINGH et al, 2013b;KUMAR et al, 2016). Hence, the present study was attempted to analyze the genetic diversity among A. racemosus accessions using RAPD markers to provide helpful genetic data for measuring the pattern of genetic diversity.…”