“…Because the allelic frequencies of CYP2C19 4 to 6 are extremely rare in East Asians (less than 0.4%) 20,21) , two CYP2C19 mutant alleles, CYP2C19 2 (rs4244285, c. 681G A, p. P227P) and 3 (rs4986893, c. 636G A, p. W212X), were investigated using the ABI SNaPshot reaction and the ABI 3100 automated genetic analyzer (Applied Biosystems, Foster City, CA, USA). PCR was carried out in a total volume of 10 L which contained 30 ng genomic DNA, 0.2 mol/L of each primer (with primer sequences available on request), 0.25 mmol/L of each dATP, dNTP, dTTP, and dGTP (Invitrogen, SanDiego, CA, USA), 0.5 units of Taq DNA polymerase, and 1.0 L of 10 X buffer contain-ing 25 mmol/L MgCl2 (Qiagen, Hilden, Germany).…”