Genetic variation of the white-spotted longicorn beetle Anoplophora spp. (Coleoptera: Cerambycidae) in Japan detected by mitochondrial DNA sequence

Abstract

Genetic variation of the white-spotted longicorn beetle Anoplophora spp., distributed on the Japanese mainland and the Ryukyu Islands, was examined using nucleotide sequences of mitochondrial DNA. Two fragments, a 1.2-kblong fragment containing portions of the cytochrome oxidase subunit I and II genes and a 1.4-kb-long fragment containing portions of 16S and 12S rDNAs, were sequenced. In phylogenetic analyses based on the sequences, 294 individuals collected at 75 localities were divided into two major groups (groups A and B) and then split into seven subgroups (A1-A4 and B1-B3). In group A, the closely related subgroups A1, A2, and A3 were mainly distributed on the Japanese mainland and were roughly separated among geographic areas, although the range of A3 spread significantly from Kyushu to the Central Ryukyu Islands. The sequence of A4, detected from one individual collected in eastern Honshu, was almost the same as that reported for A. chinensis (Forster) on the Chinese continent. In group B, subgroups B1 and B2 were restricted to the Central and Southern Ryukyu Islands, respectively, while B3 was distributed widely in both regions. Based on the results, we discuss the geographic origin of the haplotypes and movement of the insects between geographic areas.

Comparison of fecundity and longevity of Anoplophora malasiaca (Coleoptera: Cerambycidae) adults fed on three different host‐plants

“…Third, a mitochondrial DNA analysis indicated substantial differences between the two longhorn beetles (Muraji et al. ).…”

Detection and identification of citrus long-horned beetle, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) a new pest in Antalya Province, Turkey by sequencing of mtCOI region

“…They determined that the phylogenetic tree was divided into three major clades and that Anoplophora [A. glabripennis, A. chinensis and Anoplophora lurida (Pascoe, 1857)] and Monochamus [Monochamus alternatus Hope, 1842, Monochamus urussovi (Fisch., 1806 and Monochamus sutor (L., 1758)] were placed in the third lineage. Apart from such molecular studies supporting morphological studies in the Lamiinae, in another study, Muraji et al (2011) determined that the genetic variation of species belonging to the Anoplophora in Japan was identified by mtDNA sequence and the species was split into two main groups. In the same study, they noted that a sample taken in East Honshu was the same as A. chinensis from China.…”

“…In DNA extraction studies, leg parts belonging to live or just dead insects were used as described by Muraji et al (2011). DNA was extracted in accordance with the CTAB protocol for invertebrates (Folmer et al, 1994) from three individuals.…”

Degradation of white birch shelterbelts by attack of white-spotted longicorn beetle in central Hokkaido, northern Japan

“…We have used A. malasiaca as the scientific name in this study to follow the Dictionary of Japanese Insect Names (http://konchudb.agr.agr.kyushu-u.ac.jp/dji/), though A. malasiaca is often used as a synonym of A. chinensis (Haack et al 2010). DNA analysis has revealed that A. malasiaca is not necessarily the same as A. chinensis in Japan (Muraji et al 2011).…”