Genetic variation of isolated and peripheral populations of Pinus sylvestris (L.) from glacial refugia

Prus-Głowacki, Wiesław; Urbaniak, Lech; Bujas, E.; Curtu, Aleksandru L.

doi:10.1016/j.flora.2011.11.006

Cited by 33 publications

(31 citation statements)

References 37 publications

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“…Differentiation between those geographical regions indicates limited gene flow and possibly different evolutionary history. Previous genetic studies showed that the populations from Turkey, the Iberian Peninsula and Scotland were characterized by a higher level of heterozygosity or some unique molecular markers relative to the populations in central and northern Europe (Prus-Głowacki et al 2012;Floran et al 2010;Pyhäjärvi et al 2007;Wachowiak et al 2011). The intermediate genetic structure of the Scottish pine population relative to the central and marginal locations may result from the admixture of populations with different postglacial histories (Wachowiak et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Genetic characteristics of Scots pine in Poland and reference populations based on nuclear and chloroplast microsatellite markers

Hebda¹,

Wójkiewicz²,

Wachowiak³

2017

Silva Fenn.

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Polymorphisms at a set of eighteen nuclear (nSSR) and chloroplast (cpSSR) microsatellite loci were investigated in sixteen populations of Scots pine (Pinus sylvestris L.) derived from the provenance trial experiment and representative of the species distribution range and climatic zones in Poland. The patterns of genetic variation were compared to the reference samples from the species distribution in Europe and Asia. A similar level of genetic variation and no evidence of population structure was found among the Polish stands. They showed genetic similarity and homogenous patterns of allelic frequency spectra compared to the Northern European populations. Those populations were genetically divergent compared to the marginal populations from Turkey, Spain and Scotland. The population structure patterns reflect the phylogeography of the species and the divergence of populations that most likely do not share recent history. As the analysed provenance trial populations from Poland are diverged in phenotypic traits but are genetically similar, they could be used to test for selection at genomic regions that influence variation in quantitative traits.

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Section: Discussionmentioning

confidence: 99%

Genetic characteristics of Scots pine in Poland and reference populations based on nuclear and chloroplast microsatellite markers

Hebda¹,

Wójkiewicz²,

Wachowiak³

2017

Silva Fenn.

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“…As the most widely distributed member of the family Pinaceae (Critchfield & Little, 1966;Turna, 2003) in Central and Eastern Europe (including the Carpathians), Scots pine is present only in isolated, peripheral localities. Also, it has been postulated that in Europe Scots pine survived the last glaciations in five main refugia, namely the Iberian Peninsula, the north Apennine Peninsula, south of the Carpathians, the Sudetes, and the Balkans (Prus-Głowacki et al, 2012). Nowadays, these refugial populations represent gene pools of the species and are affected by isolation, mutations and selection due to ecological conditions and genetic drift.…”

Section: Introductionmentioning

confidence: 99%

Morphological and anatomical differentiation in peripheral Pinus sylvestris L. populations from the Carpathian region

Köbölkuti¹,

Tóth²,

Ladányi³

et al. 2017

Dendrobiology

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Carpathian Scots pine populations having peripheral distribution within the species' range are often sustained in specific types of habitats, such as peat bogs and rocky surfaces or lime consisting sandy substrates. Due to their long time adaptive processes, which involve genotypes that can be preadapted to a given selection pressure, historically isolated populations are subjects of particular interest in studies of in situ adaptation. In this study we focus on detecting the level of phenotypic differentiation based on cone morphology and needle anatomy in marginal populations of Pinus sylvestris L. in the Pannonian Basin and the Carpathian Mountains. Six cone morphological and eight needle anatomical characters were measured and four cone morphological and four needle anatomical ratios were calculated. Our results in concordance with paleobotanical data indicate a common origin of the populations from the Northern Carpathians and the Pannonian Basin. High levels of variation were observed in cone morphology. Discriminant function analysis based on the eight cone characteristics revealed clearly discernible groups of populations and indicated significant differentiation among populations growing in peat bogs and on rocky substrates. Significant differences among populations from different habitats were also revealed by comparing needle anatomical variables. The phenotypic differentiation by habitat type based on the measured characters might be evaluated as a sign of local adaptation with detectable phenotypic patterns.

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“…The genetic differentiation was slightly lower than in the Scots pine populations from Germany (2%) (Müller-Starck 1987) and from northeastern Europe (2.5%) (Prus-Głowacki et al 2012). However, the statistically significant differences in the G ST found between the intermediate-and late-flowering tree groups may be explained by the significant differences in the G ST for loci ShDH A and DIAF.…”

Section: Discussionmentioning

confidence: 75%

“…Our study generally confirmed Chung's results, except for ShDH locus (not used by Chung), which was significantly differentiated among the flowering groups. The recent analyses of 51 European populations of P. sylvestris showed a significant genetic differentiation at ShDH A and ShDH B loci across Europe (Prus-Głowacki et al 2012). Mean values of observed heterozygosity (Ho) for locus ShDH A in the Iberian Peninsula and Scotland were 0.421 and 0.590, respectively, whereas in Central and Eastern Europe they were 0.273 and 0.287, respectively.…”

Section: Discussionmentioning

confidence: 99%

Shikimate dehydrogenase (E.C. 1.1.1. 25 ShDH) alleles as potential markers for flowering phenology in Pinus sylvestris

Prus-Głowacki¹,

Sukovata²,

Lewandowska-Wosik³

et al. 2015

Dendrobiology

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Abstract:The aims of this study were 1) to determine the variability in the flowering phenology of Scots pine (Pinus sylvestris L.) clones in a seed orchard and 2) to compare the genetic structure and genetic markers (13 isozyme loci and 5 chloroplast and 3 nuclear DNA microsatellite loci) among groups of clones that are differentiated by flowering phenology. Using the timing of male inflorescence development, 57 plus trees represented by their clones in a seed orchard were classified into three phenological groups: early-, intermediate-, and late-flowering. The microsatellites showed no significant differences in the genetic structure of the analyzed phenological groups. However, the frequency of allele 2 at the shikimate dehydrogenase A locus (ShDH A 2) differed significantly between the groups of early-and late-flowering trees and between the groups of intermediate-and late-flowering trees. In addition, a significant difference in the frequencies of the genotype ShDH A 11 was observed between the intermediate-and late-flowering groups. Nei's genetic distance indicated that the late-flowering group was the most genetically distant among the phenological groups. These results suggest that the ShDH A locus might be considered as isoenzymatic marker that differentiates these flowering groups of Scots pine clones. At several isozyme and DNA loci, the presence of private alleles in each group of pines was observed. However, these alleles cannot serve as markers of Scots pine flowering time because of their low frequencies.

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Genetic variation of isolated and peripheral populations of Pinus sylvestris (L.) from glacial refugia

Cited by 33 publications

References 37 publications

Genetic characteristics of Scots pine in Poland and reference populations based on nuclear and chloroplast microsatellite markers

Genetic characteristics of Scots pine in Poland and reference populations based on nuclear and chloroplast microsatellite markers

Morphological and anatomical differentiation in peripheral Pinus sylvestris L. populations from the Carpathian region

Shikimate dehydrogenase (E.C. 1.1.1. 25 ShDH) alleles as potential markers for flowering phenology in Pinus sylvestris

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