Genetic Variation of H-FABP Gene and Association with Intramuscular Fat Content in Laiwu Black and Four Western Pig Breeds

Abstract

This study was performed to detect genetic variation of the heart fatty acid-binding protein (H-FABP) gene by PCR-RFLPs approach and its association with intramuscular fat (IMF) content. Data from 223 individuals, including one Chinese native pig breed and four western pig breeds, were analyzed. The results showed that for the H-FABP gene, there was one polymorphic Hinf site Ⅰ in the 5'-upstream region, whereas there were one HaeIII and one HinfI (marked as HinfI*) polymorphic site in the second intron, respectively. The three PCR-RFLPs were present in all breeds tested. The allele frequencies, however, revealed significant differences between them (p<0.05). Furthermore, the allele frequency distribution of HinfI in the Laiwu Black and that of HinfI* in the Hampshire breed were at disequilibrium, which might be the result of selective breeding. Results also indicated that for HinfI, HaeIII and HinfI* H-FABP RFLP, significant (p<0.05) contrasts of 0.78%, -0.69% and 0.72% were detected in the least square means of IMF content between the homozygous genotype HH and hh, DD and dd, BB and bb classes, respectively. It implied that the HHddBB genotype had the highest IMF content in this experimental population and these H-FABP RFLPs could serve, to some extent, as genetic markers for use in improvement of IMF content.

Risk of multi-drug resistant Campylobacter spp. and residual antimicrobials at poultry farms and live bird markets in Bangladesh

“…Emergence of MDR bacteria from poultry farms is a major threat to public health, especially in low-and middle-income countries. The poultry industry is among the rapidly growing agro-based enterprises with an annual increasing rate of 20% per annum in Bangladesh [35]. Being the major reservoirs of Campylobacter spp., poultry and livestock are mainly responsible for the ongoing spread of campylobacteriosis throughout the world.…”

Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig

“…Laiwu Pig, an excellent type of North China local fatty breed of pig, is known for high meat quality with good tenderness (Zeng et al 2005 ), bright color, and higher IMF, while it is the best animal model to study the mechanisms of high intramuscular fat deposition (Spurlock and Gabler 2008 ; Chen et al 2013 ). The Lulai Black pig is a cross of a Laiwu pig with introgression of a Large White pig.…”

Risk of multi-drug resistant Campylobacter spp. and residual antimicrobials at poultry farms and live bird markets in Bangladesh

“…Comparison of symptomatic and asymptomatic infections in individual farms indicated that diverse combinations of multiple risk factors, concerning hygiene (e.g., hand washing, and proper cleaning), poultry rearing practices, waste disposal and health management, were synergistic socio-environmental drivers of the MDR Campylobacter pathogens in poultry sector.DiscussionEmergence of MDR bacteria from poultry farms is a major threat to public health, especially in lowand middle-income countries. The poultry industry is among the rapidly growing agro-based enterprises with an annual increasing rate of 20% per annum in Bangladesh[32]. Being the major reservoirs of Campylobacter spp., poultry and livestock are mainly responsible for the ongoing spread of campylobacteriosis throughout the world.…”