GENETIC VARIATION OF ENTOMOPATHOGENIC FUNGI, Metarhizium anisopliae AND Isaria amoenerosea AND THEIR PATHOGENICITY AGAINST SUBTERRANEAN TERMITE, Coptotermes curvignathus

Kin, Pong Kuan

doi:10.21894/jopr.2017.2901.04

Cited by 5 publications

(1 citation statement)

References 12 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…anisopliae yang diperoleh dari tanah pertanian/non pertanian wilayah timur Laut Mati Turki dan kelompok yang ditentukan tidak terkait dengan tipe habitat, lokasi geografis, pertumbuhan di tempat yang berbeda suhu, resistensi UV, atau virulensi terhadap T. molitor. Pong et al (2017) juga melaporkan M. anisopliae yang diisolasi dari tanah mineral dan tanah gambut tidak berada dalam satu kelompok, ini menunjukkan bahwa jenis tanah bukan faktor yang mempengaruhi variasi genetik M. anisopliae. Pada pohon filogenetik ada temuan menarik bahwa pertanaman yang diberi tandan kosong kelapa sawit (M8 dan M15) dan limbah cair kelapa sawit (M10) berada dalam cluster yang sama.…”

Section: Profil Pita Dna Hasil Pcr Dengan Marka Rapdunclassified

Keragaman genetik cendawan entomopatogen Metarhizium anisopliae (Metsch.) berasal dari tanah pertanaman kelapa sawit berdasarkan penanda RAPD

Sirait¹,

Tobing²,

Safni³

2023

J Entomol Indones

View full text Add to dashboard Cite

Metarizhium anisopliae (Metsch.) is an entomopathogenic fungus that attacks Oryctes rhinoceros (Linnaeus). This fungus is one of the potential bioagents that is environmentally friendly. The purpose of this study was to isolate the fungus M. anisopliae from various oil palm plantation soil samples and to see its genetic diversity based on RAPD markers. The method used in this research is the morphological and the molecular identification of M. anisopliae (ITS), and genetic diversity testing using RAPD markers. Fifteen isolates of M. anisopliae isolated from 15 oil palm plantation sites in North Sumatra were examined for their molecular diversity. On the basis of morphological characteristics, all isolates were identified as M. anisopliae. The result of sequencing with the ITS primer showed that all isolates of M. anisopliae isolated from soil had a 85–99% homology of with M. anisopliae and M. brunneum reference strains from gen bank database National Center Biotechnology Information (NCBI). Eight RAPD primers generated 39 scorable bands which 38 (98,44%) of them were polymorphic. Clustering analysis was performed based on RAPD profiles using Neighbour-Joining Tree method that formed 3 groups. Key words: entomopathogenic fungi, genetic diversity, ITS sequencing, RAPD

show abstract

Section: Profil Pita Dna Hasil Pcr Dengan Marka Rapdunclassified