Genetic Variation of E. coli Strains Isolated from Poultry Slaughterhouses at Ismailia Governorate, Egypt

Abstract

Shiga toxin producing E. coli is represented as one of the main source of foodborne infectious disease distributed all over the world. This area of study not fully explained before at Ismailia governorate. So this study aimed to make genetic survey of all isolated E. coli strains with attention to shiga toxins isolated from different sections of poultry slaughterhouses and workers at Ismailia governorate. One hundred and fifty swab samples were collected from baskets, workers hands, machines, processing tools and food contact surfaces and subjected to bacteriological examination. Ten samples were positive for E. coli with a percentage 6.6%.

Phylogenetic Tracking of Ica-locus in MRSA from Different Sources at Ismailia City, Egypt

“…The incidence of Staphylococcus isolates from frozen raw chickens was low, which was consistent with the findings of (De Boer et al, 2009), who reported that imported chicken goods such as frozen filets had a low frequency of Staphylococcus. These findings differed by 2.6% from those of (Ezzat et al, 2018) , who recovered S. aureus from fresh raw chicken.…”

“…MRSA DNA was extracted from purified bacterial cells using the QIAamp DNA Mini Kit (Invitrogen, USA), and the methods were followed exactly (Ezzat et al, 2018). From the confirmed strains nuc,ica, spa, mecA, blaZ, Coa genes were detected, PCR was performed using specific sets of primers (Metabion, Germany), and the cycling conditions were performed as in (Table 1) according to (Ezzat et al, 2018). The final volume was 25 µl (12.5 µl Go Taq ® Green Master Mix 2X, µl (20 pmol) for each forward and reverse primer, 5µl sample DNA, and PCR water up to 25 µl).…”