Genetic Variation of 25 Y-Chromosomal and 15 Autosomal STR Loci in the Han Chinese Population of Liaoning Province, Northeast China

Abstract

In the present study, we investigated the genetic characteristics of 25 Y-chromosomal and 15 autosomal short tandem repeat (STR) loci in 305 unrelated Han Chinese male individuals from Liaoning Province using AmpFISTR® Yfiler® Plus and IdentifilerTM PCR amplification kits. Population comparison was performed between Liaoning Han population and different ethnic groups to better understand the genetic background of the Liaoning Han population. For Y-STR loci, the overall haplotype diversity was 0.9997 and the discrimination capacity was 0.9607. Gene diversity values ranged from 0.4525 (DYS391) to 0.9617 (DYS385). Rst and two multi-dimensional scaling plots showed that minor differences were observed when the Liaoning Han population was compared to the Jilin Han Chinese, Beijing Han Chinese, Liaoning Manchu, Liaoning Mongolian, Liaoning Xibe, Shandong Han Chinese, Jiangsu Han Chinese, Anhui Han Chinese, Guizhou Han Chinese and Liaoning Hui populations; by contrast, major differences were observed when the Shanxi Han Chinese, Yunnan Bai, Jiangxi Han Chinese, Guangdong Han Chinese, Liaoning Korean, Hunan Tujia, Guangxi Zhuang, Gansu Tibetan, Xishuangbanna Dai, South Korean, Japanese and Hunan Miao populations. For autosomal STR loci, DP ranged from 0.9621 (D2S1338) to 0.8177 (TPOX), with PE distributing from 0.7521 (D18S51) to 0.2988 (TH01). A population comparison was performed and no statistically significant differences were detected at any STR loci between Liaoning Han, China Dong, and Shaanxi Han populations. The results showed that the 25 Y-STR and 15 autosomal STR loci in the Liaoning Han population were valuable for forensic applications and human genetics, and Liaoning Han was an independent endogenous ethnicity with a unique subpopulation structure.

Genetic polymorphism and evolutionary differentiation of Eastern Chinese Han: a comprehensive and comparative analysis on KIRs

“…Using KIR as the panel of genetic marker, the results showed that Eastern Han from Jiangsu province had the closest relationship with Jilin Han, followed by Shanghai Han, indicating that Eastern Han shared a common ancestry, which was consistent to the findings based on other genetic markers, such as autosomal STRs and Y-STRs by Yao et al . 57 . The notable association of Eastern Han with Jilin Han was confirmed by the famous large-scale migration literally called “Crashing into Guandong”.…”

Meningococcal disease and control in China: Findings and updates from the Global Meningococcal Initiative (GMI)

“…Liaoning province is located in the northeast of China, with a population of approximately 43 million people. 39 Currently, the incidence rate of MD is <1 case per 100,000 population, with the majority of cases recorded during the springtime, and in the cities of Shenyang and Dalian, from 2004 to 2016. MD mainly affects individuals aged 0-15 years, while children, students and farmers (defined as laborers that travel from rural areas into cities for work) are the three main groups affected.…”

Forensic characterization of 15 autosomal STRs in four populations from Xinjiang, China, and genetic relationships with neighboring populations

“…Principal components analysis was performed with MVSP 3.1 ( http://www.kovcomp.com ) based on allelic frequencies of the 15 autosomal STR loci. Nei’s standard genetic distances between currently studied and previously published populations (Russian 20 , Saraki Pakistan 21 , Korean 22 , Punjabi 23 , Indian 24 , Morocco 25 , Eastern Turkey 26 , Hong Kong 27 , Japanese 28 , Interior Sindh (unpublished), Hungarian 29 , South Iran 30 , Azerbaijan 31 , Turkish Cypriot 32 , Afghanistan 33 , Bangladesh 34 , Malaysia 35 , Kadazan Malaysia 36 , Sindh Pakistan 37 , Iraq 38 , Pashtuns Afghanistan 39 , Tajik Afghanistan 39 , Uzbek Afghanistan 39 , Turkmen Afghanistan 39 , Mongols of Mongolia 40 , Hazara Afghanistan 39 , Kuala Lumpur Malaysia 41 , Miao 42 , East Mongolia of China 43 , Dong 44 , Bouyei 45 , Han Liaoning 46 , Manchu Liaoning 47 , Hui Qinghai 48 , Uyghur China 49 , Russian in China 50 , Dongxiang Qinghai 51 , Salar Qinghai 51 , Kazakh China 13 ) were generated using the Phylip 3.69 package 52 and visualized with Mega7 software 53 .…”