Genetic variation of 10 X chromosomal STR loci in Indian population

Abstract

X chromosomal short tandem repeats have the potential to complement the analyses of the autosomal, Y chromosomal, and mitochondrial DNA markers in forensics and population genetics, and extensive research on X chromosomal markers is being carried out. In the present study, a decaplex for the co-amplification of ten X chromosomal microsatellite loci (DXS6807, DXS8378, DXS7132, DXS6809, DXS6789, DXS101, DXS7133, GATA172D05, HPRTB, and GATA31E08) was optimized and 749 blood samples of unrelated male individuals from the four major linguistic families of India were analyzed. The number of alleles for the studied loci ranged from 7-16 while the gene diversity values varied from 0.408 to 0.855. Two new alleles were observed for the loci DXS101 and HPRTB. Statistical parameters of forensic interest were calculated and all loci were found to be polymorphic. High power of discrimination was observed for the loci DXS101, DXS6809, and DXS6789. The present study demonstrates the efficacy of these X-linked markers for human identification and kinship analysis.

Refining the genetic portrait of Portuguese Roma through X‐chromosomal markers

“…However, recent work focusing on X-chromosomal diversity in India (Mukerjee et al, 2010) reported no signs of LD for a set of 10 X-STR markers, seven of which were analyzed in this study. To address this issue, it would be essential to have data for all of the investigated loci in the populations that were involved in the process of gene flow.…”

“…Unfortunately, the complete set of markers studied here was not available for Indian populations. However, recent work focusing on X-chromosomal diversity in India (Mukerjee et al, 2010) reported no signs of LD for a set of 10 X-STR markers, seven of which were analyzed in this study.…”

“…Indian samples were also used for comparison (Mukerjee et al, 2010, Ghosh et al, 2011. All control individuals were of non-Gypsy origin and were born and living in different regions of Portugal.…”

“…All control individuals were of non-Gypsy origin and were born and living in different regions of Portugal. Indian samples were also used for comparison (Mukerjee et al, 2010, Ghosh et al, 2011. More information on the markers and references for the samples used in this study is displayed in Supporting Information Tables S1-S3.…”

“…While this comparison was impossible for the full set of markers studied here, the strategy was applied to recently released data for Indian populations (Mukerjee et al, 2010;Ghosh et al, 2011) that contained 7 of the 10 X-STRs of this work plus the entire set of 15 autosomal STRs. While this comparison was impossible for the full set of markers studied here, the strategy was applied to recently released data for Indian populations (Mukerjee et al, 2010;Ghosh et al, 2011) that contained 7 of the 10 X-STRs of this work plus the entire set of 15 autosomal STRs.…”