Genetic Variation in VP7 Gene of Human Rotavirus Serotype 1 (G1 Type) Isolated in Japan and China

Xin, Ke-Qin; Morikawa, Shigeru; Fang, Zhao-Yin; Mukoyama, Atsushi; Okuda, Kenji; Ushijima, Hiroshi

doi:10.1006/viro.1993.1663

Virology

1993

DOI: 10.1006/viro.1993.1663

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Genetic Variation in VP7 Gene of Human Rotavirus Serotype 1 (G1 Type) Isolated in Japan and China

Ke-Qin Xin

Shigeru Morikawa

Zhao-Yin Fang

et al.

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Cited by 39 publications

(33 citation statements)

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“…Sequence analysis has also revealed the existence of genetic lineages within the P and G types. For example, the strains of type P [8] form at least three lineages (23,29,36), and four lineages of type G1 have been found so far (15,29,30,36,55). Genetic clustering is also evident at least for segments 5 (16), 7 (44), 8 (41), and 10 (12,17,28).…”

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confidence: 90%

Frequent Reassortments May Explain the Genetic Heterogeneity of Rotaviruses: Analysis of Finnish Rotavirus Strains

Maunula

Bonsdorff

2002

J Virol

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The predominant rotavirus electropherotypes (e-types) during 17 epidemic seasons (1980 through 1997) in Finland were established, and representative virus isolates were studied by nucleotide sequencing and phylogenetic analysis. The virus isolates were either P[8]G1 or P[8]G4 types. The G1 and G4 strains formed one G1 lineage (VP7-G1-1) and one G4 lineage, respectively. Otherwise, they belonged to two P[8] lineages (VP4-P[8]-1 and -2) unrelated to their G types. Phylogenetic analysis of partial sequences of all 11 RNA segments obtained from the strains also revealed genetic diversity among gene segments other than those defining P and G types. With the exception of segments 1, 3, and 10, the sequences of the other segments could be assigned to 2 to 4 different genetic clusters. The results of this study suggest that, in addition to the RNA segments encoding VP4 and VP7, the other RNA segments may segregate independently as well. In total, the 9 predominant e-types represented 7 different RNA segment combinations when the phylogenetic clusters of their 11 genes were determined. The extensive genetic diversity and number of e-types among rotaviruses are best explained by frequent genetic reassortment.

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confidence: 90%

Frequent Reassortments May Explain the Genetic Heterogeneity of Rotaviruses: Analysis of Finnish Rotavirus Strains

Maunula

Bonsdorff

2002

J Virol

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“…In addition, we have reported molecular analysis of human G 1, G2 and G3 rotaviruses in Japan, China and Pakistan (20,21,23). In our recent study, G3 rotaviruses were considered to be intraserotypically more heterogeneous than G I and G2 viruses.…”

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confidence: 99%

Molecular Characterization in the VP7, VP4 and NSP4 Genes of Human Rotavirus Serotype 4 (G4) Isolated in Japan and Kenya

Kudo

Zhou

Cao

et al. 2001

Microbiology and Immunology

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The VP7, VP4 and NSP4 genes of human rotavirus serotype 4 (G4) were analyzed to investigate intraserotypic variations. The techniques used included reverse transcription polymerase chain reaction with subtype specific primers, restriction fragment length polymorphism analysis and sequence analysis. Twelveisolates (nine from Japan and three from Kenya) and two standard strains (Hoehi, Odelia) were G4A P[8] Wa group NSP4. A standard strain (ST3) was G4A P[6] Wa group NSP4 and a strain (VA70) was G4B P[8] Wa group NSP4. These results show G4 rotaviruses can be divided into three combinations at the moment.Key words: Rotavirus, Serotype G4, VP7 gene, VP4 gene, NSP4 gene Rotavirus is a major etiologic agent that causes diarrhea in infants and children worldwide (3). In developing countries, rotavirus infection, coupled with malnutrition, leads to a severe and fatal outcome (1). At present, the genus Rotaviridae is classified into seven groups (groups A to G), based on group specific antigen. Group A rotavirus is the most prevalent (18,22). The genome of rotavirus comprises of 11 segments of double-stranded RNA, each of which encodes a distinct viral protein. Two outer capsid proteins, VP4 and VP7, are involved in virus neutralization. The neutralization specificities of VP4 and VP7 are designated as P and G serotypes for protease-sensitive protein and glycoprotein, respectively (5). Currently, two overlapping classification systems are used for VP4. P serotypes can be determined by P serotype specific monoclonal antibodies, however VP7 comprises of the greater percentage of the outer capsid protein and as such hyper-immune antisera raised to intact viral particles primarily recognize VP7 rather than VP4. For this reason, P typing is usually performed using reverse transcription polymerase chain reaction

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“…In addition, we have reported molecular analyses of human G1, G2 and G3 rotaviruses in Japan, China and Pakistan (17,18,21). In our recent study on G3 human rotaviruses, the five Chinese isolates were found to be genetically distant from 16 Japanese isolates and SAll (18).…”

mentioning

confidence: 97%

Genetic Variation in the VP4 and NSP4 Genes of Human Rotavirus Serotype 3 (G3 Type) Isolated in China and Japan

Cao

Akihara

Fang³

et al. 1999

Microbiology and Immunology

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Sequence analyses of the VP4 and NSP4 genes were performed on twenty human isolates of serotype G3 rotavirus obtained from China and Japan. One isolate from China, CHW17, possessed P [4] genotype VP4 and KUN group NSP4 genes which are associated with G2.One isolate (02/92) from Japan, which was shown to have a wider spacing between RNA segments 10 and 11 by RNA polyacrylamide gel electrophoretic analysis like AU-1, possessed P[9] genotype VP4 and AU-1 group NSP4 genes. The other isolates had P[8] genotype VP4 and Wa group NSP4 genes. While the nucleotide sequence conservation among the G3 VP7 genes was more than 79% (Wen et al, Arch. Virol., 1997, 142: 1481-1489, the conservation of VP4 and NSP4 genes in the same genotypes or groups was more than 85%.

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Genetic Variation in VP7 Gene of Human Rotavirus Serotype 1 (G1 Type) Isolated in Japan and China

Cited by 39 publications

References 0 publications

Frequent Reassortments May Explain the Genetic Heterogeneity of Rotaviruses: Analysis of Finnish Rotavirus Strains

Frequent Reassortments May Explain the Genetic Heterogeneity of Rotaviruses: Analysis of Finnish Rotavirus Strains

Molecular Characterization in the VP7, VP4 and NSP4 Genes of Human Rotavirus Serotype 4 (G4) Isolated in Japan and Kenya

Genetic Variation in the VP4 and NSP4 Genes of Human Rotavirus Serotype 3 (G3 Type) Isolated in China and Japan

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