Genetic variation in the porcine myogenin gene locus

Soumillion, A.; Erkens, J.H.F.; Lenstra, Johannes A.; Rettenberger, G.; Pas, M.F.W. te

doi:10.1007/s003359900504

Cited by 48 publications

(56 citation statements)

References 33 publications

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“…Genetic influence of the MRF gene family on porcine muscle development has been shown before. Genetic variation in porcine myogenin was related to muscle and general body growth (Soumillion et al, 1997;te Pas et al, 1999a ) while myf-5 did not show such a relationship (te Pas et al, 1999b ). Furthermore, postnatal expression levels of myf-5, MyoD, and myogenin were related to growth rate (te Pas et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Pas

Wit

Priem

et al. 2005

J Muscle Res Cell Motil

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Myogenesis, the formation of muscle fibers, is a complex process. Pigs have been selected for efficient muscle growth for the past decades making them interesting to study myogenesis. We studied expression profiles of genes known to affect myogenesis, muscle structural proteins, and energy metabolism in prenatal pigs from 14 to 91 days of gestation. Primary and secondary muscle fiber formation takes place during days 30-60 and 54-90 of gestation, respectively. Differential expression and expression levels of the genes were studied using microarray technology. Gene activation and repression profiles were studied counting the number of spots with detectable signal. The number of spots for muscle tissue structural protein genes showing upregulated expression increased constantly from day 14 until day 91of gestation indicating continued activation of genes during this period. The mRNA expression level of the genes showed a peak around day 35 of gestation. The expression levels of genes affecting myogenic differentiation (stimulating and inhibiting) showed a peak at day 35 of gestation. The number of spots for differentiation-stimulating genes showing differential expression reaches a first peak around day 35 of gestation and a nadir at day 49 of gestation while the number of spots for differentiation-inhibiting genes reaches a nadir at day 35 of gestation. Myogenic differentiation seems less a matter of the expression level of genes affecting differentiation, but depends on the balance between the number of significantly activated genes for stimulating and inhibiting differentiation. Genes stimulating myoblast proliferation showed a small peak expression prior to day 35 of gestation indicating myoblast proliferation before differentiation. The number of spots and the expression levels of genes for glycolysis and ATP-metabolism are at a nadir around days 35 and 49-63 of gestation suggesting that the energy metabolism is low during fusion of myoblasts into multinucleated muscle fibers.

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Section: Introductionmentioning

confidence: 99%

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Pas

Wit

Priem

et al. 2005

J Muscle Res Cell Motil

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“…Contrary to the expectations resulting from previously published studies (Verner et al, 2007;Civaňova and Knoll, 2007;Stupka et al, 2012) all of the MYF4 genotypes showed only non-significant (P<0.05) impact on selected production traits. The associations of MYOD genes family with pork quality has been previously investigated in various pig populations by several studies (Soumillion et al, 1997;Kapelañski et al, 2005;Cinar et al, 2012;Stupka et al, 2014). The MyoD genes were tested as candidate genes with an expected, significant effect principally on the muscle deposition (Kłosowska et al, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…Subsequently, the concentration and purity of genomic DNA were analysed based on the spectrophotometry measurements by the optical density at wave length of 260 nm. The polymorphism in MYF4 gene was analysed according to Soumillion et al (1997) using restriction endonuclease MspI. The products of PCR reaction and restriction fragments have been separated and visualised using horizontal electrophoresis in 2% agarose gels (130 V for 50 min) and stained with day GelRed.…”

Section: Methodsmentioning

confidence: 99%

“…The expression of the MYF5 and MYOD1 (MYF3) genes plays a fundamental role during myoblast proliferation, while the expression of the MYOG (MYF4) and the MYF6 (MRF4) genes is linked to the differentiation and maturation of myofibres (Stupka et al, 2012). The myogenin (MYF4) gene, which controls the start of myoblast fusion and myofibres formation, was identified on the 9 th autosome, in the 9q2.1-2.6 area (Soumillion et al, 1997;Ernst et al 1998). The myogenin is a transcription factor specific to skeletal muscle and fulfils a key function in muscle differentiation by controlling the onset of myoblast fusion and the establishment of myofibres.…”

Section: Introductionmentioning

confidence: 99%

“…Genetic variation in the MYF4 gene can be associated with differences in myoblast and myofibril numbers (Weisz et al, 2011). Ernst et al (1993) and Soumillion et al (1997) detected using endonuclease MspI gene three polymorphic sites in the MYF4, in the promoter region, the second intron and the 3' side of the gene.…”

Section: Introductionmentioning

confidence: 99%

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Carcass and Meat Quality in Relation to the Polymorphism in Porcine Myf4 Gene

Trakovická¹,

Moravčíková²,

Navrátilová³

et al. 2016

AgricultForest

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SUMMARYThe aim of this study was to analyse the associations between polymorphism in porcine myogenin gene (MYF4) and economically important traits in relation to the carcass and meat quality of pigs. The genomic DNA samples were taken from in total 180 crossbreeds (Large White x Landrace). The detection of polymorphism in MYF4 gene were performed in order to evaluate its effect on back fat thickness, proportion of valuable meat parts, MLT area and proportion of thigh. The genotyping of analysed individuals was carried out by means PCR-RFLP method and restriction endonuclease MspI. The allele frequencies were as follows: A 0.75 and B 0.25. A prevalence of AA genotype (59%) compared to AB (33%) and BB genotypes (8%) were detected in analysed crossbreed population. The observed average value of heterozygosity (0.33) and positive value of the Wright's F IS index (0.12) similarly reflected the higher proportion of homozygous genotype in populations. The effect of MYF4 gene polymorphism on selected phenotypic traits has been tested using one-way ANOVA procedure. The statistical analysis showed only non-significant results. Due to the polygenic character of selected phenotypic traits the involvement of other candidate genes and increase of sample size could clarify the role of MYF4 gene in porcine carcass and meat quality regulation. The study, which is based on molecular variability of livestock genetic resources, is necessary for the genetic improvement and understanding of relations between markers and trait of interest.

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Polymorphisms in coding and regulatory regions of the porcineMYF6 andMYOG genes and expression of theMYF6 gene inm. longissimus dorsi versus productive traits in pigs

et al. 2006

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MYOG and MYF6 belong to the MyoD gene family. They code for the bHLH transcription factors playing a key role in later stages of myogenesis: differentiation and maturation of myotubes. Three SNPs in porcine MYF6 and two in porcine MYOG were analysed in order to establish associations with chosen carcass quality and growth rate traits in Polish Landrace, Polish Large White and line 990 sows. No statistically significant effect of SNP in the promoter region of the MYF6 gene on its expression measured on mRNA level was found. Associations between the genotype at the MYF6 locus and carcass quality traits appeared to be breed-dependent. The C allele in the case of SNP in the promoter region and GC haplotype in exon 1 were advantageous for right carcass side weight in Polish Landrace sows and disadvantageous for this trait in Polish Large White sows. These gene variants were also the most advantageous for loin and ham weight in sows of line 990. The mutation in exon 1 of the MYOG gene had no statistically significant association with carcass quality traits and the mutation in the 3'-flanking region had the breed-dependent effect as well. These results suggest that SNPs analysed in this study are not causative mutations, but can be considered as markers of some other, still unrevealed genetic polymorphism that influences the physiological processes and phenotypic traits considered in this study.

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Genetic variation in the porcine myogenin gene locus

Cited by 48 publications

References 33 publications

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Transcriptome Expression Profiles in Prenatal Pigs in Relation to Myogenesis

Carcass and Meat Quality in Relation to the Polymorphism in Porcine Myf4 Gene

Polymorphisms in coding and regulatory regions of the porcineMYF6 andMYOG genes and expression of theMYF6 gene inm. longissimus dorsi versus productive traits in pigs

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