Genetic variation in the coffee berry disease pathogen, Colletotrichum kahawae

Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil

“…The high frequency of nit3 mutant that we observed was also found in previous research on Colletotrichum species (Brooker et al, 1991;Beynon et al, 1995), although some studies reported a predominance of nit1 mutants (Nitzan et al, 2002;Pereira, 2005;Fávaro et al, 2007). The recovery of nit phenotypes (especially nitM) from Colletotrichum species is clearly not straightforward and sometimes is not possible (Beynon et al, 1995;Varzea et al, 2002).…”

“…The characterization of VCGs has been employed successfully in various species of Colletotrichum (Brooker et al, 1991;Skroch et al, 1992;Wasilwa et al, 1993;Correll et al, 1993;Chacko et al, 1994;Beynon et al, 1995;Gichuru et al, 2000;Nitzan et al, 2002;Varzea et al, 2002;Abang et al, 2004;Pereira, 2005), and nitrate non-utilizing mutants have been used to demonstrate heterokaryosis and parasexual segregation in C. lindemuthianum isolates (Castro-Prado et al, 2007). However, little could be inferred about vegetative compatibility in this species, because the nit mutants were obtained from only five isolates.…”

Responses of Compact Coffee Clones Against Coffee Berry and Coffee Leaf Rust Diseases in Tanzania

“…Further studies within C. kahawae at the DNA level have also revealed limited polymorphism (Beynon et al, 1995). The formation of hyphal fusions during infection of C. kahawae is a likely mechanism creating pathogen variations as there is involvement of an exchange of genetic materials (Mwang'ombe et al, 1992).…”

“…Inside the paper bag, green infected berry samples were sandwich between layers of newspaper and kept in a cool box at 18 to 20 ºC for the pathogen to be viable for successful subsequent isolation (Johnston & Booth, 1983). Colletotrichum kahawae was isolated from the survey samples using the method described by Beynon et al (1995). Infected green coffee berries with CBD lesions were surface sterilized by immersing for 15 min in a 5% solution of sodium hypochlorite (available chlorine 0.5% W/V) and rinsed in three changes of sterile distilled water.…”

“…Infected coffee berry tissues were then removed aseptically and incubated on distilled water agar at 22 ºC. Hyphae from advancing edges of the tentative C. kahawae colonies were then transferred to Malt Extract Agar (MEA) (Beynon et al, 1995). The cultures were incubated at 22 ºC for 10 days to allow colony growth.…”