Genetic variation in Symbiodinium isolates from giant clams based on random-amplified-polymorphic DNA (RAPD) patterns

Baillie, Brett K.; Belda-Baillie, C. A.; Silvestre, Víctor Quipuscoa; Sison, M.; Gómez, Alvarado; Gomez, Enrique D.; Monje, Virginia D.

doi:10.1007/s002270000290

Cited by 63 publications

(70 citation statements)

References 26 publications

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“…Studies on bleached reefs have indicated that clade D increases as a result of bleaching (Glynn et al 2001, Baker et al 2004, Berkelmans & van Oppen 2006, Jones et al 2008, LaJeunesse et al 2009), but long-term environmental history, particularly high temperature with low temperature variability, is also likely to play an important role in determining the relative abundance of clade D (Baker et al 2004, Fabricius et al 2004, McClanahan et al 2007, La Jeunesse et al 2010, Oliver & Palumbi 2011, al though over large scales these pattern may be obscured by other biogeographic factors (Oliver & Palumbi 2009). Clade-level diversity in Symbiodinium has been reported in clams previously (Rowan et al 1996, Rowan 1998, Baillie et al 2000a, 2000b, Sison 2003, but the distribution of this diversity over ecological or biogeographic scales has not been reported. …”

Section: Maxima Hosts Containing Multiplementioning

confidence: 99%

“…The second most common symbiont (A6) has been recorded in Tridacna from the Philippines (Baillie et al 2000b) and Japan (LaJeunesse et al 2004). It has not been recorded in other hosts and consequently also appears to be specific to tridacnid clams.…”

Section: Symbiont Diversity In Tridacnid Clams From Indonesiamentioning

confidence: 99%

“…lower light levels) that could favor different symbiont types. Although to date no studies have investigated spatial variability in Symbiodinium within individual giant clams, the evidence demonstrating that individuals can routinely host multiple symbiont types (Baillie et al 2000b, Carlos et al 2000, Ishikura et al 2004 suggests that this is possible. If different symbionts are not evenly distributed throughout clam tissues, then the fact that individual hosts were partially biopsied, rather than sacrificed in their entirety, suggests that symbiont diversity within individuals may be higher than reported here.…”

Section: Mixed Symbiodinium Communities In Clam Hostsmentioning

confidence: 99%

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Patterns of Symbiodinium distribution in three giant clam species across the biodiverse Bird’s Head region of Indonesia

DeBoer¹,

Baker²,

Erdmann³

et al. 2012

Mar. Ecol. Prog. Ser.

110

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The formation and persistence of modern coral reefs depends largely on organisms that host dinoflagellate algal symbionts of the genus Symbiodinium. There are important ecological and physiological differences among Symbiodinium types, and many host species are able to associate with multiple types, which may facilitate adaptation to local environmental change. Using denaturing gradient gel electrophoresis (DGGE) and sequencing of internal transcribed spacer-2 (ITS2) ribosomal DNA, we identified 11 Symbiodinium types belonging to clades A, C, and D in 250 host animals from 3 Tridacna species in eastern Indonesia. Individuals with multiple symbiont types were common: 42% of all clams had symbionts from multiple clades and 15% of all clams had multiple types from a single clade. T. crocea associated more often with clade C symbionts and less frequently with clade D symbionts. T. squamosa associated more frequently with clade D and less often with clade C symbionts. T. maxima did not preferentially associate with a particular Symbiodinium clade, but sample sizes were low. We used both satellite sea surface temperature and in situ recordings to characterize the thermal environment in the study area. Clams with clade C and D symbionts were located in areas with higher mean temperatures, while clams with clade A symbionts were in cooler areas. This is consistent with previous research indicating that clade C and D types may be more heat-tolerant than clade A. These results support the hypothesis that giant clams can associate with different symbiont types based on local environmental conditions. KEY WORDS: Symbiodinium · Tridacna spp. · Thermal tolerance · Climate change · Coral reef · IndonesiaResale or republication not permitted without written consent of the publisher

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Section: Maxima Hosts Containing Multiplementioning

confidence: 99%

Section: Symbiont Diversity In Tridacnid Clams From Indonesiamentioning

confidence: 99%

Section: Mixed Symbiodinium Communities In Clam Hostsmentioning

confidence: 99%

See 1 more Smart Citation

Patterns of Symbiodinium distribution in three giant clam species across the biodiverse Bird’s Head region of Indonesia

DeBoer¹,

Baker²,

Erdmann³

et al. 2012

Mar. Ecol. Prog. Ser.

110

View full text Add to dashboard Cite

show abstract

“…Studies of other DNA regions such as internal transcribed spacer (ITS) regions of nuclear DNA (Baillie et al 2000, LaJeunesse 2001, van Oppen et al 2001, the 23S rDNA of chloroplasts (Santos et al 2002, Pochon et al 2006, psbA minicircle (Barbrook et al 2006), mitochondrial sequences (Takabayashi et al 2004), and microsatellites , Magalon et al 2006 support the cladal relationships based on ssRNA and lsRNA and provide within-clade resolution. Most reports have identified Symbiodinium at the clade level.…”

Section: Introductionmentioning

confidence: 99%

Worldwide biogeography of Symbiodinium in tropical octocorals

Goulet

Simmons²,

Goulet³

2008

Mar. Ecol. Prog. Ser.

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Although octocorals are important components of coral reefs, most research on the genetic diversity of symbiotic zooxanthellae (Symbiodinium spp.) has focused on scleractinian (stony) corals. For both groups, most geographic comparisons have occurred within the same ocean or only included a few geographic sites. We characterized the genetic diversity of Symbiodinium in tropical octocorals in 15 geographic areas throughout the world. By combining octocoral samples analyzed in this study with published data, the cladal identities of zooxanthellae in 117 octocoral species were identified and a global analysis was performed. For 63 octocoral species, the Symbiodinium type was also identified. The vast majority (87.2%) of octocorals hosted a single zooxanthella clade. Most octocorals also hosted specific Symbiodinium types. A biogeographic pattern emerged. Octocorals from 4 geographic areas in the Indo-west Pacific hosted Symbiodinium C. In the Great Barrier Reef, 11 octocoral species (24.4%) hosted Symbiodinium D, 3 species hosted Symbiodinium G, and 1 species each hosted Symbiodinium A and B. Red Sea octocorals hosted predominantly Symbiodinium C, with Symbiodinium A occurring in 3 species. In Hawaii, 2 octocorals hosted Symbiodinium C and 1 hosted Symbiodinium A. In Bermuda, only clade B was found. In the Caribbean, unlike in the Indo-Pacific, the dominant Symbiodinium was B, predominantly type B1, with Symbiodinium C occurring in a few species. Understanding the similarities and differences between octocoral and scleractinian coral symbioses with zooxanthellae may enable predictions of which symbioses will survive in periods of environmental change.KEY WORDS: Symbiodinium · Zooxanthellae · Octocorals · Corals · GIS · Clade · RFLP · BiogeographyResale or republication not permitted without written consent of the publisher

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“…Their symbionts are mostly placed into Symbiodinium clade A, C, and occasionally D (Kirkendale and Paulay, 2017; see comprehensive literature list in Supplementary Table 2) and a single host individual can sometimes possess multiple symbiont lineages (DeBoer et al, 2012). Within Fraginae, the symbiont community from four species have been examined to date using molecular markers: Fragum fragum (Linnaeus, 1758), Fragum unedo (Linnaeus, 1758), Corculum cardissa, and Corculum monstrosum (Gmelin, 1791) (Carlos et al, 1999(Carlos et al, , 2000Baillie et al, 2000;LaJeunesse, 2001;Dreier et al, 2014). Symbionts freshly isolated from the hosts mostly belong to Symbiodinium clade C, while symbionts cultured from host tissues are from clade A (Carlos et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Characterizing Photosymbiosis Between Fraginae Bivalves and Symbiodinium Using Phylogenetics and Stable Isotopes

Volsteadt

Kirkendale

et al. 2018

Front. Ecol. Evol.

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Photosymbiotic associations between heterotrophic hosts and photosynthetic algae play crucial roles in maintaining the trophic and structural integrity of coral reef ecosystems. The marine bivalve subfamily Fraginae contains both non-symbiotic and photosymbiotic lineages, making it an ideal comparative system to study the origin and evolutionary adaptations of photosymbiosis. The symbiotic species exhibit unique morphological adaptations to photosymbiosis. However, the basic biology of these photosymbiotic relationships, such as symbiont diversity and nutritional benefits, has not been thoroughly characterized. In this study, we examined the general morphology of four Fraginae species occupying different depths (0-10 m): Corculum cardissa, Fragum fragum, Fragum scruposum, and Fragum sueziense. Abundant symbionts were found in the mantle, gill, and part of the foot, contained in tubular networks within host tissues. We used molecular phylogenetics to investigate the algal symbiont community of these Fraginae species. Results showed that symbionts from all four species are dinoflagellates belonging to the Symbiodinium clade C and we did not detect any host-specific or geographic-specific genetic structures within the symbionts. We also used stable carbon isotope analyses to examine whether the cockles are directly utilizing photosynthetically derived carbon sources. All species show less depleted 13 C compared to filter-feeding bivalves, suggesting at least part of their organic carbon is derived directly from the symbionts. However, 13 C depletion of Fragum sueziense collected from deeper habitats are less distinguishable from filter-feeding bivalves. This indicates that species in deeper habitats may rely less on photosymbiosis due to the reduced light availability. Given that the symbiotic fragines exhibit varying morphologies, habitats, and utilization of symbiont photosynthesis, the subfamily represents an ideal model system to study differential adaptations to photosymbiosis.

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Genetic variation in Symbiodinium isolates from giant clams based on random-amplified-polymorphic DNA (RAPD) patterns

Cited by 63 publications

References 26 publications

Patterns of Symbiodinium distribution in three giant clam species across the biodiverse Bird’s Head region of Indonesia

Patterns of Symbiodinium distribution in three giant clam species across the biodiverse Bird’s Head region of Indonesia

Worldwide biogeography of Symbiodinium in tropical octocorals

Characterizing Photosymbiosis Between Fraginae Bivalves and Symbiodinium Using Phylogenetics and Stable Isotopes

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