Genetic variation in neutrophil accumulation in mice is not mediated through immigrant regulatory cells

Abstract

SUMMARYGenetic variation of induced peritoneal neutrophiiia in mice was accompanied by parallel variation in macrophage responses. The timing of the macrophage responses in high responder (C57B1/10) mice indicated a potential role for these cells in mediating the enhanced neutrophil response. However, adoptive transfer of inflammatory macrophages did not induce neuirophilia. Analysis of peritoneal cytokine levels in high and low responder mice further indicated [hat IL-I. lL-3. GM-CSE, G-CSE and interferon-gamma (IFN-7) were not involved in mediating the genetic variation observed. Exogenous tumour necrosis factor-alpha (TNF-a) was effective in inducing the high responder phenotype. despite the absence of detectable TNF-fi in either peritoneal fluid or serum. A role for genetically determined differential expression of endotheiial adhesion molecules in high and low responders is suggested.

Low-Dose Irradiation Programs Macrophage Differentiation to an iNOS+/M1 Phenotype that Orchestrates Effective T Cell Immunotherapy

“…Eight-to 12-week-old, sex-matched C3H donor mice received a 2 Gy TBI or were left unirradiated. After 1 day, 1 ml of thioglycolate (3%, AppliChem; Marley et al, 1995) were injected intraperitoneally (i.p.). Peritoneal macrophages were collected by peritoneal lavage after 3 days and 5 3 10 6 doses were injected i.v.…”

N-Glycans Differentially Regulate Eosinophil and Neutrophil Recruitment during Allergic Airway Inflammation

“…Previous studies have demonstrated decreased total leukocyte recruitment to the peritoneum in Mgat5 Ϫ/Ϫ mice after exposure to TG (31). However, it is important to note that the end point of the study as well as the mouse strain were different compared with the current study (3 h versus 6 h), and strain-related differences are known to exist during induced neutrophilia in mice (32). In the TG-exposed Mgat5 Ϫ/Ϫ mice in our study, we did not observe a significant reduction in eosinophil recruitment compared with WT counterparts (p ϭ 0.1), although the trend in reduction paralleled the BALF eosinophils in the allergen challenge model (Fig.…”