Genetic variation in leaf and stem glucosinolates in resynthesized lines of winter rapeseed (Brassica napus L.)

Abstract

Glucosinolates are secondary components characteristic for the Brassicaceae with complex biological functions. Glucosinolates in the seeds are anti-nutritive when feeding animals and their inheritance have been extensively investigated. Much less is known about the genetics of glucosinolates in leaves and stems, which may attract some insects, while repelling others. They may also inhibit bacterial processes of importance when using green biomass for the production of biogas. The objective of this study was to analyse the genetic variation of total and individual glucosinolates in the green material of rapeseed. For this 28 resynthesized winter rapeseed lines were tested at two locations. There was a large variation in leaf glucosinolate content between 0.10 and 4.75 lmol/g dry matter. The predominant leaf glucosinolates are the alkenyle glucosinolates progoitrin, gluconapin and glucobrassicanapin. The line R53 is exceptional, while combining a relative high content of the indole glucosinolate glucobrassicin with low alkenyle glucosinolates in the leaves. The total glucosinolate concentration in the stems and leaves is not correlated with the seed glucosinolate concentrations. Heritabilities are above h 2 = 0.60 for progoitrin, h 2 = 0.65 for gluconapin, h 2 = 0.30 for glucobrassicanapin and h 2 = 0.52 for total glucosinolate content in the leaves. In conclusion, resynthesized rapeseed is an important genetic resource to modify the leaf glucosinolate content and composition of rapeseed.

Fire and Brimstone: Molecular Interactions between Sulfur and Glucosinolate Biosynthesis in Model and Crop Brassicaceae

“…In different tissues of Brassica , aliphatic GSLs account for 70–97% of the total GSL content. This has been reported in leaves of B. oleracea (Cartea et al, 2008), leaves and stems of B. napus (Cleemput and Becker, 2012), leaves and seeds of B. juncea (Shilpa et al, 2012; Othmane, 2015) and sprouts and mature leaves of B. rapa (Wiesner et al, 2013). Aliphatic GSLs are mostly derived from Met, with a few from leucine, isoleucine, alanine, and valine.…”

Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence

“…Overall, rapeseed breeding suffers from low genetic diversity, especially for the Canola type (Dupont et al, 1989). Artificially resynthesized rapeseed has been a solution for introducing genetic variation (Becker et al, 1995;Cleemput and Becker, 2012;Girke et al, 2012). Given our understanding of the asymmetric evolution of rapeseed subgenomes, selection of appropriate cabbage parents with a relatively wild C genome is of particular interest for increasing genetic variation in rapeseed.…”

Performance of cabbage stem flea beetle larvae (Psylliodes chrysocephala) in brassicaceous plants and the effect of glucosinolate profiles

“…Glucotropeolin was added as an internal standard in the analyses. The samples were treated with sulfatase and, after incubation, the desulphoglucosinolates were analysed by HPLC, as described by Cleemput & Becker (2012).…”