Genetic Variation in Hypothalamic-Pituitary Axis Candidate Genes and Their Effects on Milk Production Traits in Iranian Holstein Cattle

Sadeghi, Mostafa; Mokhber, M.; Shahrbabak, M. Moradi

doi:10.1134/s1022795422110096

Cited by 4 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results indicated that the genotype frequencies for POU1F1/Hinf1 were in balance (P>0.05), likely due to the closed barn environment, absence of external migration, and controlled breeding practices. Similarly in our study, it has been reported that POU1F1/HinfI genotype frequencies are in Hardy-Weinberg equilibrium in Holstein calves, Anatolian Black Steppe, and Turkish Grey cattle (Ozdemir et al, 2018;Cobanoglu and Ardicli, 2022;Fındık and Özdemir, 2022;Sakar and Zülkadir, 2022;Sadeghi et al, 2022), and similarly, genotype distributions are balanced in Holstein Friesian, Brown Swiss, and Simmental cattle breeds (Mohammadabadi et al, 2015;Aytekin and Bayraktar, 2022). On the other hand, in different cattle breeds such as Grati-Ongole, Bos Indicus, and Bali cattle (Bos javanicus), it has been reported that Pit-1 genotype distributions are not in Hardy-Weinberg equilibrium and there are deviations in HW equilibrium (Jakaria and Noor, 2015;Hartati et al, 2018).…”

Section: Discussionsupporting

confidence: 86%

“…Similar studies on different breeds, such as buffaloes, Slovak Simmental, and Holstein cattle, have also reported no significant association between these genotypes and milk yield (Heidari et al, 2012;Ebrahimi et al, 2015;Trakovická et al, 2015;Ozdemir et al, 2018;Thuy et al, 2018;Zghair and Hassooni, 2021). However, studies conducted in the Horasan region of Iran reported that the POU1F1/HinfI genotype polymorphism is linked to milk composition in Holstein cattle, affecting protein and fat percentages, and in Shawial cattle, affecting total milk yield and 300-day milk yield (Chauhan et al, 2015;Mohammadabadi et al, 2015;Sadeghi et al, 2022) Further research with larger sample sizes and more diverse breeds is necessary to better understand this relationship.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle

SÖNMEZ,

ÜNAL

2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

View full text Add to dashboard Cite

As the global population grows, there is a need to produce higher yields in food, agriculture, and livestock. To achieve this, scientists are exploring new techniques and methods. However, it is crucial to select the right candidate genes and markers, especially in techniques like QTL and MAS in livestock, to ensure success. We conducted a study to determine allele frequencies and their association with milk yield in 70 Simmental cattle breeds in two lactations using the PCR-RFLP technique. The statistical analysis was conducted using the general linear model procedure with the least square method The study focused on the allele frequencies for the POU1F1-HinfI gene in Simmental cattle. The dominant B allele frequency for POU1F1/Hinf1 was 0.58, while the A allele frequency was 0.42. Simmental population was under HardyWeinberg Equilibrium (HWE) for the POU1F1-HinfI genotypes (p>0.05) The study found that genotype frequencies were in balance for POU1F1/Hinf1. No significant correlation between POU1F1-HinfI gene polymorphisms and milk yield was found, but they have been associated with growth and reproductive traits in various cattle breeds. The results could provide useful information for breeding programs aimed at improving the performance traits of Simmental cattle.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle

SÖNMEZ,

ÜNAL

2023

Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi

View full text Add to dashboard Cite

show abstract

IGF-I and GH Genes polymorphism and their association with milk yields, composition and reproductive performance in Holstein–Friesian dairy cattle

Saleh,

Hassan,

EL-Hedainy

et al. 2024

BMC Vet Res

View full text Add to dashboard Cite

Background The insulin-like growth factor (IGF-I) and growth hormone (GH) genes have been identified as major regulators of milk yield and composition, and reproductive performance in cattle. Genetic variations/polymorphism in these genes have been found to influence milk production, yield and quality. This investigation aimed to explore the association between IGF-I and GH polymorphisms and milk yield and composition, and reproductive performance in a herd consisting of 1000 Holstein–Friesian (HF) dairy cattle from El-Alamia farm. The experimental animals were 76 ± 7.25 months in age, with an average live weight of 750 ± 50.49 kg, and raised under the same conditions of feeding and weather. The studied animals were divided into three categories; high producers (n = 280), medium producers (n = 318) and low producers (n = 402). Results The digestion of 249 bp for IGF-I-SnaBI using the Restriction-fragment-length-polymorphism (RFLP) technique yielded two alleles; T (0.59) and C (0.41) and three genotypes; TT (0.52), TC (0.39) and CC (0.09) and this agrees with the results of DNA/gene sequencing technique. The sequencing analysis of the IGF-I gene revealed polymorphism in position 472 (C > T). Nucleotide sequencing of the amplified fragment of the IGF-I gene of different genotypes was done and submitted to the NCBI GenBank with Accession no. MH156812.1 and MH156811.1. While the digestion of 432 bp for GH-AluI using the RFLP technique yielded two alleles; A (0.81) and G (0.19) and two genotypes; AA (0.77) and AG (0.23) and this agrees with the results of DNA/gene sequencing technique. The sequencing analysis of the GH gene revealed polymorphism in the position 1758 C > G and in turn led to changes in amino acid sequence as Alanine for (A) compared to Glycine for (G). Nucleotide sequencing of the amplified fragment of the GH gene was done and submitted to the NCBI GenBank with Accession no. MH156810.1. The results of this study demonstrate the effects of variants of the GH-IGF-I somatotrophic axis on milk production and composition traits in commercial HF cattle. The greatest values of milk yield and reproductive performance were observed on IGF-I-SnaBI-TC and GH-AluI-AG genotypes. While the greatest % fat and % protein values were observed on IGF-I-SnaBI-CC and GH-AluI-AA genotyped individuals. Conclusion The genetic variation of the studied genes can be utilized in selecting animals with superior milk yield, composition and reproductive performance in Holstein–Friesian Dairy Cattle under subtropical conditions.

show abstract

Gene polymorphism of the family of transcription factors and their influence on the milk productivity of the first heifers

Tkachenko,

Fairushina,

Kleshcheva

2024

jour

View full text Add to dashboard Cite

Purpose: this study investigated the polymorphism of transcription activators genes and their effects on milk production traits.Materials and methods. The first time in the Ural region, studies were carried out to determine the polymorphism of cattle in the STAT1 and STAT5A genes and the study of their associations with dairy productivity. Allele polymorphism was determined by the КASP method – Кompetitive Allele Specific PCR.Results. 92 cows were genotyped for the STAT1 gene locus, three genovariants were identified – STAT1СС (69,5 %), STAT1СТ (27,2 %) и STAT1ТТ (3,3 %). In the first lactation, the productivity of cows with the STAT1СС genotype was: milk yield 8379 kg, milk fat content 4.18 %, milk protein content 3.23 %. The individuals with STAT1СТ genotype had milk yield 8045 kg, MF 4.17 %, MP 3.23 %. And the individuals with genotype STAT1ТТ had milk yield 7855 kg, MF 4.16 %, MP 3.18 %. The advantage of cows with the STAT1CC genotype was 334-524 kg of milk compared to analogues from other groups. The genotypes of 90 cows were determined by the locus of the STAT5A gene. The STAT5ACT genotype is widespread among the studied livestock (51.1 %), the STAT5AСС genotype occurs with a frequency of 25.6 %, STAT5ATT genotype – with a frequency of 23.3 %. The productivity of cows with the STAT5ACC genotype was: milk yield of 8376 kg of milk, fat content of 4.19 %, protein content of 3.21%. The milk yield of the first heifers in the STAT5ATT and STAT5ACT groups is lower by 83–142 kg, the fat content is lower by 0.02–0.03 %. According to the results of the study, the STAT1 gene has an advantage for use as markers of high dairy productivity of Holstein cattle.

show abstract

Genetic Variation in Hypothalamic-Pituitary Axis Candidate Genes and Their Effects on Milk Production Traits in Iranian Holstein Cattle

Cited by 4 publications

References 44 publications

The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle

The Relationship of POU1F1-HinfI Gene Polymorphisms on Milk Yields in Simmental Cattle

IGF-I and GH Genes polymorphism and their association with milk yields, composition and reproductive performance in Holstein–Friesian dairy cattle

Gene polymorphism of the family of transcription factors and their influence on the milk productivity of the first heifers

Contact Info

Product

Resources

About