Genetic Variation in Experimental Dental Caries in Four Inbred Strains of Rats

Kanamoto, T.; Nonaka, Kenichi; Nakata, M.

doi:10.1159/000261638

Cited by 5 publications

(5 citation statements)

References 9 publications

(9 reference statements)

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“…Our study revealed that children who have a familial history of dental caries are more at risk (OR 8.2) of dental caries. A similar association was reported by various other authors [ 90 , 91 , 92 ]. A correlation of dental decay in siblings was observed by Klein and Palmer (1938) [ 93 ].…”

Section: Discussionsupporting

confidence: 92%

Prevalence of Dental Caries in Children in Mymensingh and Its Associated Risk Factors: A Cross-Sectional Study

et al. 2022

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Background: Children suffer from various oral and periodontal diseases. Dental caries is one of the most prevalent oral diseases among children in the world. This study was conducted to identify the prevalence and risk factors of dental caries in children in Mymensingh, Bangladesh. Methods: A cross-sectional study was conducted on 362 pediatric patients who attended the Dental Unit of Mymensingh Medical College from March to September 2019. The sample size was calculated using a statistical formula and the children were selected using a systematic random sampling technique. Children and their guardians were interviewed and data were recorded using a structured questionnaire. Risk factors were analyzed using multivariate logistic regression. Results: The overall prevalence of dental caries was 82.7%. The prevalence of caries was significantly higher in aged children (8–10 years) and also in rural, low-income, and illiterate families. Seven significant risk factors were identified that included residence in the rural area (OR: 7.31 [1.73–30.83]), a parental income of BDT ≤ 20,000 per month (OR: 4.75 [1.49–15.05]), reduced duration (≤1 min) of teeth cleaning (OR: 18.54 [2.05–168.17]), teeth cleaning before breakfast (OR: 93.30 [10.95–795.32]), the spoon-feeding method (OR: 12.57 [2.09–75.61]), long-term (37–48 months) breastfeeding (OR: 212.53 [8.69–5195.25]), and family oral problem (OR: 8.20 [2.57–26.16]). Conclusions: The prevalence of dental caries among the children in Mymensingh is very high and was associated with residence in rural areas, parental income, reduced duration of teeth cleaning, teeth cleaning before breakfast, the spoon-feeding method, long-term breastfeeding, and family oral problems.

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Section: Discussionsupporting

confidence: 92%

Prevalence of Dental Caries in Children in Mymensingh and Its Associated Risk Factors: A Cross-Sectional Study

et al. 2022

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“…Nevertheless, to date, there is little literature describing the direct effects of AMPs on dental caries. Prior to clinical trials, the well-established rat model is widely used for its favourable susceptibility to caries akin to that of humans, with the small body size of rats optimizing operations and a high reproducibility [22,23]. In addition, the physicochemical properties and macromolecular composition of saliva could influence microbial colonization [24,25] and the maintenance of antibacterial agents [26] both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

Wang

Zeng

et al. 2018

Journal of Oral Microbiology

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Background:Targeting Streptococcus mutans, the principle cariogenic bacterium, could prove to be an effective means of preventing dental caries. A de novo designed antimicrobial peptide, GH12, has shown bactericidal effects on S. mutans and inhibitory effects on virulence and regulatory systems of S. mutans. However, the effects of GH12 on caries remain to be elucidated.Objectives: The objectives of this study were to evaluate the direct effects of GH12 on caries and numbers of S. mutansin vivo.Design: The Keyes score was evaluated in a rodent model and in vivo S. mutans was quantified by qPCR. To further interpret the in vivo anticaries efficacy, an in vitro biofilm model using short-term treatments with GH12 mimicked treatments in vivo was adopted. Results: In vivo data showed that GH12 at 8 mg/L reduced the incidence and severity of caries. Furthermore, GH12-treated animals had less S. mutans infection. In vitro data revealed that GH12 reduced the number of S. mutans within the biofilm, as well as reducing lactic acid and water-insoluble EPS synthesis of S. mutans biofilms. Afterwards, all rats showed no significant difference in weight gain, and no sign of harm to the mucosa, indicating that GH12 possessed good biocompatibility in vivo.Conclusion: Due to the combined inhibitory effects of GH12 on the biomass and cariogenic properties of S. mutans biofilms, the population of S. mutans in vivo was reduced, residual S. mutans biofilms were less acidic and compact, and thereby the onset and development of caries were arrested. Such findings collectively certified the clinical prospects for GH12. ARTICLE HISTORY

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“…At this stage, the main strategies are experimental: application of animal models and controlled crossbreeding, and observational: applicable to human populations, such as familial aggregation and twin studies. Typically, animal studies use inbred strains presenting extreme known phenotypes to analyze the result of controlled crossing, correlating genotypes and phenotypes (Kanamoto et al , 1994). Familial aggregation analysis investigates the clustering of disease cases in large pedigrees resulting from excessive sharing of genetic and/or environmental variants (Burton et al , 2005; Hopper et al , 2005).…”

Section: Genetic Analysis Of Complex Traitsmentioning

confidence: 99%

“…Genetic studies have been conducted to better comprehend the genetic component associated with the individual susceptibility to dental decay development. The approaches which have been used include: (i) experimental studies involving animal models and controlled crossbreeding (Hunt et al , 1944, 1955; Kanamoto et al , 1994); (ii) observational studies involving human populations, such as familial aggregation analysis (Garn et al , 1976a) and twin studies (Finn and Caldwell, 1963; Boraas et al , 1988; Conry et al , 1993; Sofaer, 1993; Hassel, 1995; Shuler, 2001), and (iii) linkage and association studies (Slayton et al , 2005; Bagherian et al , 2008; Deeley et al , 2008; Patir et al , 2008; Vieira et al , 2008).…”

Section: Genetic Analysis Of Dental Cariesmentioning

confidence: 99%

“…However, when the mice were exposed to a high cariogenic diet, the difference between the two lines decreased (Larson, 1965; Larson et al , 1968). In the 1990s, Kanamoto et al (1994) also demonstrated the genetic influence in caries scores of molar teeth, when comparing four inbred strains of male mice which were inoculated with S. mutans and fed with a cariogenic diet. Other studies started to identify genomic regions and polymorphisms related to susceptibility and/or resistance variations (Kurihara et al , 1991; Quivey et al , 2005).…”

Section: Genetic Analysis Of Dental Cariesmentioning

confidence: 99%

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A critical review: an overview of genetic influence on dental caries

2010

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Dental decay is a complex, chronic disease and one of the most common illnesses in dentistry today. Several dental decay risk factors have been identified during the last years; however, these variables alone may not entirely explain the disease development. Genetic research applied to dental decay began in the 1930s with experimental reports in animals and human observational research. Only recently, have some studies begun to search for genetic polymorphisms in humans and apply linkage analysis. However, due to the complex characteristics of the disease, the strong influence from several biological and environmental factors, and the small number of genetic studies related to dental caries, the genetic basis still requires further study. Therefore, the aim of this review is to provide a brief description of the current methodology for genetic analysis of complex traits, followed by a comprehensive evaluation of the literature related to genetic susceptibility/resistance to dental decay and a discussion of different aspects of the applied methodology. Advances towards the elucidation of the dental decay genetic basis may contribute to the understanding of the disease etiopathogenesis and to the identification of high risk groups, thus providing potential targets for effective screening, prevention and treatment.

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Genetic Variation in Experimental Dental Caries in Four Inbred Strains of Rats

Cited by 5 publications

References 9 publications

Prevalence of Dental Caries in Children in Mymensingh and Its Associated Risk Factors: A Cross-Sectional Study

Prevalence of Dental Caries in Children in Mymensingh and Its Associated Risk Factors: A Cross-Sectional Study

Antimicrobial peptide GH12 targetsStreptococcus mutansto arrest caries development in rats

A critical review: an overview of genetic influence on dental caries

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