Genetic Variation in Bovine Neutrophil Sensitivity to Glucocorticoid Challenge

Tempelman, Robert J.; Saama, P.M.; Freeman, A.E.; Kelm, S.C.; Kuck, A.L.; Kehrli, Marcus E.; Burton, Jeanne L.

doi:10.1080/090647002762381078

Cited by 10 publications

(16 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This marked L-selectin down-regulation on circulating cells may have been supported by interactions of the blood neutrophils with vascular endothelial cells, which is well known to cause L-selectin shedding during vascular margination [1,2,3]. In fact, demargination likely accounted for a majority of the early rise in circulating neutrophils with modestly reduced surface L-selectin expression we observed in the 3-and 6-h groups [32,50,51]. However, in the 12-and 24-h groups, where neutrophilia peaked, continued massive loss of surface L-selectin on circulating cells must have been a result of effects of DEX on blood neutrophils themselves, possibly related to increased longevity of existing neutrophils [43,[53][54][55] and (or) direct inhibition of L-selectin gene expression (see below).…”

Section: Discussionmentioning

confidence: 62%

See 1 more Smart Citation

Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils

Weber¹,

Toelboell²,

Chang³

et al. 2004

Journal of Leukocyte Biology

View full text Add to dashboard Cite

One anti-inflammatory action of glucocorticoids is down-regulation of surface L-selectin on circulating neutrophils. However, it is unclear if this is a result of release of affected bone marrow neutrophils or if the steroid has direct effects on L-selectin expression in existing blood neutrophils. We recently demonstrated that circulating neutrophils from cattle with high blood concentrations of endogenous glucocorticoid had reduced L-selectin mRNA, suggesting that the steroid interrupted L-selectin gene expression. In the current study, dexamethasone (DEX) was administered to cattle in vivo, and blood and bone marrow neutrophils were studied simultaneously within the animal to determine which pool of cells responds to glucocorticoids with inhibited L-selectin expression. Purified blood neutrophils were also treated with DEX +/- RU486 in vitro, and glucocorticoid effects on L-selectin expression were determined. Our results indicate that glucocorticoid-induced suppression of L-selectin, which accompanies neutrophilia, is likely mediated by direct effects of glucocorticoid receptor activation on intracellular reservoirs of L-selectin mRNA and protein in cattle, predominantly in blood neutrophils.

show abstract

Section: Discussionmentioning

confidence: 62%

“…The 12 remaining steers not treated with DEX were processed as a fifth 0-h (control) group. This large number of control steers was used to help account for animal variation in basal expression of neutrophil surface Lselectin and L-selectin mRNA, which is not present following DEX administration in cattle [25,32].…”

Section: Animals and Glucocorticoid Treatmentsmentioning

confidence: 99%

Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils

Weber¹,

Toelboell²,

Chang³

et al. 2004

Journal of Leukocyte Biology

View full text Add to dashboard Cite

show abstract

“…Polish researchers identified a genetically determined polymorphism in leukocyte acid phosphatase that produces an increase in neutrophil count along with a decrease in enzyme activity 34,35 . A 2002 study found a genetic component to both the percentage of neutrophils in healthy bulls and how much that percentage increases in response to dexamethasone 36 . An association between neutrophil count and various bovine lymphocyte antigens exists, but the differences are relatively small 37,38 .…”

Section: Discussionmentioning

confidence: 99%

Comparison of bovine hematology reference intervals from 1957 to 2006

George

Snipes

Lane

2010

Veterinary Clinical Pathol

102

View full text Add to dashboard Cite

show abstract

“…Development of physiological tests that examine the susceptibility of cows to fatty liver could help determine the genetic basis of fatty liver independent of milk production. A similar approach has been used to examine the genetic basis of immune function (Tempelman et al, 2002).…”

Section: Genetic Risk Factors For Fatty Livermentioning

confidence: 99%

Invited Review: Pathology, Etiology, Prevention, and Treatment of Fatty Liver in Dairy Cows

Bobe

Young

Beitz

2004

Journal of Dairy Science

691

714

View full text Add to dashboard Cite

Fatty liver (i.e., hepatic lipidosis) is a major metabolic disorder of many dairy cows in early lactation and is associated with decreased health status and reproductive performance. In severe cases, milk production and feed intake are decreased. Therefore, a practical preventative or an efficacious treatment of fatty liver could save millions of dollars yearly in treatment, replacement, and production losses for dairy farmers. Fatty liver develops when the hepatic uptake of lipids exceeds the oxidation and secretion of lipids by the liver, which usually is preceded by high concentrations of plasma NEFA mobilized from adipose tissue. Excess lipids are stored as triacylglycerol in the liver and are associated with decreased metabolic functions of the liver. Liver can be categorized into normal liver or mild, moderate, or severe fatty liver; the latter can be subdivided further into nonencephalopathic severe fatty liver and hepatic encephalopathy. Insufficient or unbalanced dietary intake, obesity, and elevated estrogen concentrations are involved in the etiology of fatty liver, which is associated with greater incidence of dystocia, diseases, infections, and inflammations. Because even mild fatty liver is associated with decreased health status and reproductive performance of dairy cows, prevention of fatty liver by supplying cows with sufficient nutrients and a clean and health-promoting environment in the peripartal period would reduce production losses of cows more than would any treatment of fatty liver. This, however, might not be enough for cows that are obese or do not eat well, had calving difficulties or twins, have metabolic or infectious diseases, or are in severe negative energy balance because of high milk production immediately after calving. Potential and commonly used preventatives, as well as treatments, are discussed in the review. Currently, detection of fatty liver is possible only by minor surgery. Ultrasonic techniques offer a potential tool to noninvasively detect fatty liver. Future gene-array and proteomic studies may provide means to detect early molecular events in the etiology of fatty liver plus their connection with immune function and reproductive performance so that more effective treatments and preventatives of fatty liver can be developed. Such advances hopefully will make fatty liver a problem of the past.

show abstract

Genetic Variation in Bovine Neutrophil Sensitivity to Glucocorticoid Challenge

Cited by 10 publications

References 43 publications

Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils

Mechanisms of glucocorticoid-induced down-regulation of neutrophil L-selectin in cattle: evidence for effects at the gene-expression level and primarily on blood neutrophils

Comparison of bovine hematology reference intervals from 1957 to 2006

Invited Review: Pathology, Etiology, Prevention, and Treatment of Fatty Liver in Dairy Cows

Contact Info

Product

Resources

About