Genetic Variation at Five STR Loci (D10S2325, D13S325, D20S470, D18S51, and D19S253) in Korean Population

Abstract

Allele and genotype frequencies of the five STR loci (D10S2325, D13S325, D20S470, D18S51, and D19S253) were determined. Specimens were collected from unrelated volunteer blood donors from a population of Chungnam Province in South Korea (n = 130). DNA was obtained from the blood specimens using the Wizard genomic DNA purification kit (Promega, USA) and amplified using the multiplex PCR (triplex amplification of D10S2325, D13S325, and D20S470; duplex amplification of D18S51 and D19S253). The amplified DNAs were separated by denaturing polyacrylamide gel electrophoresis and then visualized by the silver staining (1). Exact x2-test for Hardy-Weinberg equilibrium and pairwise disequilibrium were analyzed using the GDA program written by Lewis P.O. and Zaykin D (2). The chance of exclusion of combined system was 0.9986.

Genetic polymorphism of 14 non-CODIS STR loci for forensic use in southeast China population

“…Frequencies of the alleles of D12S391 versus the German, Central Poland, Portugal populations indicated statistically significant differences (P < 0.0001). In contrast, the distribution pattern of alleles was very similar as in Spain [14] and Other Asian Korean populations, especially Koreans [12,13,15].…”

“…Comparison of the allele frequencies from the studied population with the European and other Asian populations for which the data were available showed the following: the distribution of the alleles of D7S1517, D2S1360 and D10S2325 was similar as in German [10] and Central Poland [11] populations (P-values ranging from 0.115 to 0.708). No statistically significant differences in frequencies of the alleles of D10S2325, D16S3253, D18S1270, D19S253 and D20S470 versus two Korean populations [12,13] were observed.…”