Genetic variation and genetic structure of Aedes aegypti population Dengue Hemorrhagic Fever (DHF) vector in West Sumatera

Abstract

Aedes aegypti is the main vector of dengue virus in the tropics and subtropics. Prevention of dengue depends on vector control due to the vaccine is still in development, understanding the survival of the species can be determined by tracking the genetic variations among individuals and determine the genetic structure of Aedes aegypti population. The purpose of this study was to analyze genetic variation and genetic structure of populations of Ae. aegypti in four dengue-endemic region in Sumatra Barat. All collected sample was performed DNA extraction to obtain DNA genomic of Ae. aegypti. DNA was amplified by using microsatellite primers resulted from previous study. Homozygous and heterozygous alleles were detected by using PAGE. Genetic variation and genetic differentiation were high in four dengue-endemic regions in West Sumatra. He scores ranged from 0.7886 to 08,404, I score were 1.5215 to 1.7263, FST score was 0.1205, and Nm was 1.8240. Genetic variation and gene flow of Ae. aegypti among populations, so that is easy for mosquito to adapt. There were two groups of Ae. aegypti resulted based on UPGMA analysis, and there was no correlation between genetic distance and geographical distance. Genetic variation of Ae. aegypti in some endemic areas of dengue fever in West Sumatra was high with moderate genetic differentiation. There were two groups of population based on genetic structure. There was no correlation between genetic distance and geographic distance.