Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis

Asemota, Helen N.; Ramser, Juliane; López-Peralta, Cristina; Weising, Kurt; Kahl, Günter

doi:10.1007/bf00037118

Cited by 48 publications

(30 citation statements)

References 33 publications

(49 reference statements)

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“…rotundata was also studied using isozyme and morphological markers (Hamon and Toure, 1990;Zoundjihekpon et al, 1994;Dansi et al, 1999Dansi et al, , 2000aMignouna et al, 2002). Accessions of these species were also evaluated with random amplified polymorphic DNA, amplified fragment length polymorphism (Asemota et al, 1996;Dansi et al, 2000b;Mignouna et al, 2003), and simple sequence repeat markers (Scarcelli et al, 2005;Obidiegwu et al, 2009a). Mignouna et al (2005) concluded that cultivars that were classified as D. cayenensis should be considered as a taxon that was separate from D. rotundata.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure and diversity in the Dioscorea cayenensis/D. rotundata complex revealed by morphological and isozyme markers

et al. 2014

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ABSTRACT.Of the 600 known yam species, only 10 are utilized as food, and the Dioscorea cayenensis/D. rotundata species complex is among the most cultivated. In Brazil, these species are commercially cultivated in the northeast region and are cultivated in the south and southeast regions as subsistence crops by traditional agriculturists. This study aimed to evaluate the genetic diversity of 21 local varieties of D. cayenensis and 2 D. rotundata accessions using 7 isozymic loci and 24 morphological markers, and to investigate the diversity distribution in different levels of organization, such as swidden fields and communities of Vale do Ribeira. Cluster analyses for both the isozymic and morphological data separated the 2 D. rotundata accessions from the D. cayenensis accessions from Vale do Ribeira. The analysis with morphological characteristics showed the presence of 2 subgroups (Iguape and Cananéia) within group I, which included all of the local varieties from Vale do Ribeira; this result may indicate the influence of the cultural units on the morphological variation. Molecular analysis of variance indicated that most of the isozymic variability was concentrated among swiddens within communities (42.5%) and within communities (40.3%). Most of the morphological variability was also concentrated among swidden fields within communities (44.8%). The correlation between geographic and genetic distances indicated that neither morphological (r = 0.17) nor isozymic diversity (r = -0.15) is structured in space. Thus, the traditional agriculturists of Vale do Ribeira maintain and manage a great diversity of D. cayenensis varieties in their communities.

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Section: Introductionmentioning

confidence: 99%

Genetic structure and diversity in the Dioscorea cayenensis/D. rotundata complex revealed by morphological and isozyme markers

et al. 2014

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“…The isozymatic genetic characterization in D. alata was performed by Lebot et al (1998) and Twyford (1990). This species was also studied with molecular markers such as RAPD, for the evaluation of resistance to anthracnose (Mignouna et al, 2002a) and genetic diversity studies (Asemota et al, 1996;Cheng and Liu, 1996;Zannou et al, 2009); AFLP, for the study of intraspecific genetic diversity (Egesi et al, 2006;Malapa et al, 2005) and development of a genetic map and analysis of QTL aimed at resistance to anthracnose (Mignouna et al, 2002b). Recently, Arnau et al (2009) used six microsatellite primers to study the segregation patterns from four different progenies of D. alata.…”

Section: Introductionmentioning

confidence: 99%

Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil

Bressan¹,

Neto²,

Zucchi³

et al. 2011

Sci. agric. (Piracicaba, Braz.)

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Traditional growers of the Vale do Ribeira, São Paulo State, grow and make use of several D. alata landraces. This study assessed the genetic diversity of 16 landraces, using isozymatic and morphological markers and comparing them with 19 commercial varieties of D. alata. Their distribution in different levels of organization such as households and communities of the Vale do Ribeira was evaluated. Isozymatic analyses were performed with polyacrylamide (six systems) and starch gels (one system), while the morphological analyses were carried out with 24 traits. Due to the polyploid nature of this species, the isozymatic bands were scored as binary data. Morphological traits were also scored as binary data. Principal coordinates and cluster analyses were conducted for both markers, using for the later the Jaccard´s similarity coefficient and UPGMA method. The separation of the landraces from the commercial varieties, which showed lower genetic diversity, was reported for both markers. No correlation between genetic and geographical distances was found for both data, which suggests that the observed variability is not spatially structured. Also, no correlation was found between both markers. The analysis of molecular variance (AMOVA) indicated that genetic diversity was mainly found within households for both isozymatic (54%) and morphological (70%) markers. The results obtained for both markers revealed the importance of traditional agriculturists in the Vale do Ribeira in maintaining high diversity for D. alata, even higher than the varieties commercialized in São Paulo State, contributing for the in situ/on farm conservation of this crop.

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“…1). Other DNAbased molecular markers, such as simple sequence repeat (Muthamia et al, 2013 andNorman et al, 2012), random amplified polymorphic DNA, and amplified fragment length polymorphism (Asemota et al, 1996;Dansi et al, 2000;and Mignouna et al, 2003), were also used in the genetic diversity studies of yam.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity Study of <i>Dioscoreas</i> Using Morphological Traits and Isozyme Markers Analyses

Efisue¹

2016

Nig. J. Biotechnol

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Genetic variation and cultivar identification of Jamaican yam germplasm by random amplified polymorphic DNA analysis

Cited by 48 publications

References 33 publications

Genetic structure and diversity in the Dioscorea cayenensis/D. rotundata complex revealed by morphological and isozyme markers

Genetic structure and diversity in the Dioscorea cayenensis/D. rotundata complex revealed by morphological and isozyme markers

Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil

Genetic diversity Study of <i>Dioscoreas</i> Using Morphological Traits and Isozyme Markers Analyses

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