Genetic Variation Among European Strains of Saccharomyces paradoxus: Results from DNA Fingerprinting

Наумова, Е. С.; Naumov, G. I.; Molina, Francis I.

doi:10.1016/s0723-2020(00)80049-1

Cited by 32 publications

(19 citation statements)

References 29 publications

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“…Individuals of the Saccharomyces stricto sensu complex sibling species isolated from Amarone wine were also genetically separated by (GTG) 5 primer (Torriani et al 1999). Moreover, strains of S. paradoxus were identified and grouped accordingly to geographical origin using (GTG) 5 primer (Naumov et al 2000). Similarly, strain differentiation has been achieved upon investigation of representative isolates belonging to species in the Williopsis sensu stricto complex (Naumova et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…PCR-based methods are advantageous over other expensive and time-consuming molecular methods like karyotyping and RFLP. RAPD is one of the simplest methods and, although problems with reproducibility may compromise the reliability of the results, it has been recommended for yeast typing (Gomes et al 2000;Naumov et al 2000). Nevertheless, the use of more stable markers is advisable.…”

Section: Introductionmentioning

confidence: 99%

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Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Silva-Filho

Santos

Resende

et al. 2005

Antonie Van Leeuwenhoek

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Yeast population used in industrial production of fuel-ethanol may vary according to the plant process condition and to the environmental stresses imposed to yeast cells. Therefore, yeast strains isolated from a particular industrial process may be adapted to such conditions and should be used as starter strain instead of less adapted commercial strains. This work reports the use of PCR-fingerprinting method based on microsatellite primer (GTG)5 to characterize the yeast population dynamics along the fermentation period in six distilleries. The results show that indigenous fermenting strains present in the crude substrate can be more adapted to the industrial process than commercial strains. We also identified new strains that dominate the yeast population and were more present either in molasses or sugar cane fermenting distilleries. Those strains were proposed to be used as starters in those industrial processes. This is the first report on the use of molecular markers to discriminate Saccharomyces cerevisiae strains from fuel-ethanol producing process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Silva-Filho

Santos

Resende

et al. 2005

Antonie Van Leeuwenhoek

View full text Add to dashboard Cite

show abstract

“…A number of biotyping procedures have been developed [2,3]. The extreme variability of the killer phenomenon [4^6] has been proposed in more recent years as a solution for the discrimination of yeasts below the species level [7^11].…”

Section: Introductionmentioning

confidence: 99%

Differential growth inhibition as a tool to increase the discriminating power of killer toxin sensitivity in fingerprinting of yeasts

Buzzini

2000

FEMS Microbiology Letters

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A panel of 27 cell-free crude killer toxin preparations were used in fingerprinting 45 Saccharomyces cerevisiae and 11 Saccharomyces exiguus strains. The differential sensitivity to different mycocins was evaluated both as binary data matrix (presence^absence of killing effect), and by considering the growth inhibition areas (measured by agar diffusion well bioassay). The first approach gave an individual fingerprinting of 68% of sensitive strains, whereas the second gave a total and reproducible (P 6 0.01) discrimination of all tested strains. ß

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“…Individuals of the Saccharomyces stricto sensu complex sibling species isolated from Amarone wine were also genetically separated using (GTG) 5 primer (Torriani et al 1999). Moreover, strains of S. paradoxus were identified and grouped accordingly to geographical origin using (GTG) 5 primer (Naumov et al 2000). Similarly, strain differentiation has been achieved upon investigation of representative isolates belonging to species in the Williopsis sensu stricto complex (Naumova et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…PCRbased methods are advantageous over other expensive and time-consuming molecular methods like karyotyping and RFLP. RAPD is one of the simplest methods and, although problems with reproducibility may compromise the reliability of the results, it has been recommended for yeast typing (Gomes et al 2000;Naumov et al 2000). Nevertheless, the use of more stable markers is advisable.…”

Section: Introductionmentioning

confidence: 99%

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Silva-Filho¹,

Santos

Resende

et al. 2005

Antonie Van Leeuwenhoek

View full text Add to dashboard Cite

Yeast populations used in industrial production of fuel-ethanol may vary according to the plant process conditions and to the environmental stresses imposed on yeast cells. Therefore, yeast strains isolated from a particular industrial process may be adapted to such conditions and should be used as the starter strain instead of less adapted commercial strains. This work reports the use of a PCR-fingerprinting method based on microsatellite primer (GTG) 5 to characterize the yeast population dynamics during the fermentation period in six distilleries. The results show that indigenous fermenting strains present in the crude substrate can be more adapted to the industrial process than commercial strains. We also identified new strains that dominated the yeast population and were more commonly present either in molasses or sugar cane fermenting distilleries. Those strains were proposed to be used as starters in those industrial processes. This is the first report on the use of molecular markers to discriminate Saccharomyces cerevisiae strains from the fuel-ethanol producing process.

show abstract

Genetic Variation Among European Strains of Saccharomyces paradoxus: Results from DNA Fingerprinting

Cited by 32 publications

References 29 publications

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

Differential growth inhibition as a tool to increase the discriminating power of killer toxin sensitivity in fingerprinting of yeasts

Yeast population dynamics of industrial fuel-ethanol fermentation process assessed by PCR-fingerprinting

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