Genetic variation among Clostridium perfringens isolated from food and faecal specimens in Lagos

Chukwu, Emelda E.; Nwaokorie, Francisca O.; Coker, A. O.; Ávila-Campos, Mário Júlio; Ogunsola, Folasade

doi:10.1016/j.micpath.2017.08.031

Cited by 8 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we differentiated the C. perfringens isolates into five distinct groups (O1-O5) and observed an appropriate discriminatory index of 0.73. In contrast with our findings, Chukwu et al [19] found 44.7% of the C. perfringens isolates were typeable using PCR-based genotyping with arbitrary OPA-3 primers. Llanco et al [46] found all (100%) of the C. perfringens isolates typeable, which is in agreement with our findings.…”

Section: Discussioncontrasting

confidence: 99%

“…These differences may be because of strong variations in hygienic and sanitary conditions of handling, processing and distribution of the products [32]. However, the results of the C. perfringens prevalence rate in the raw meat samples showed that, regarding the lower prevalence rate, there is still a high risk with respect to contamination with this pathogen in raw meat products due to poor hygiene and low levels of sanitation practices that have been used during raw meat processing and distribution [19,31]. The results in this study also showed that the prevalence rate of C. perfringens was significantly (p < 0.05, chi-square test) higher in raw whole meat samples than that in minced meat samples.…”

Section: Discussionmentioning

confidence: 99%

“…Several common genotyping assays have been used to type C. perfringens isolates from food samples, including multiple-locus variable-number tandem repeat analysis (MLVA), multi-locus sequence typing (MLST), amplified polymorphic DNA (RAPD), pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), arbitrary primer PCR-based (AP-PCR) and toxinotyping methods [14,15]. There are limited studies to characterize the antibiotic resistance, presence of toxin genes and genetic relatedness in C. perfringens isolated from raw meat samples [16][17][18][19]. Therefore, the purpose of this study was to determine the prevalence rate, antimicrobial susceptibility, toxinotype and genetic diversity in C. perfringens isolates from raw whole and minced meat samples.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Hassani

Pakbin

Brück³

et al. 2022

Antibiotics

View full text Add to dashboard Cite

Background: Clostridium perfringens is one of the highest prevailing spore-forming foodborne pathogens, which is widely distributed and causes severe disease and outbreaks in humans and animals. Raw meat and poultry are the main vehicles of this pathogen. In this study, we investigated the prevalence, antibiotic resistance pattern, toxin-encoding genes and genetic diversity of C. perfringens isolates from raw whole and minced meat samples purchased from local markets in Qazvin city, Iran (the source of beef cattle production was also located in Qazvin city, Iran). Methods: We used conventional culture-based and Kirby–Bauer disk diffusion and conventional and arbitrary primer PCR methods. Results: A total of 18 C. perfringens strains were isolated from 133 raw meat samples (13.53%). Up to 44.4 and 55.5% of these isolates were detected in raw minced and whole meat samples, respectively. We found that 72.2, 66.6, 61.1, 37.8 and 33.3% of the C. perfringens isolates were resistant to ampicillin, tetracycline, amoxicillin, ciprofloxacin and chloramphenicol antibiotics, respectively. Multidrug resistance was found in 38% of the isolates. Among the four main toxin genes evaluated, the Cpa gene was detected in all isolates, and 61.1% of the isolates were mostly recognized as type A C. perfringens. High levels of genetic diversity were observed among the isolates, and they were classified into five distinct groups. Conclusions: The isolates from whole meat samples were more resistant to antibiotics. However, toxin genes were more detected in the isolates from minced meat samples. Our findings suggest that contamination of raw meat products with multidrug resistant C. perfringens could be regarded as one of the concerning pathogens in these products. Comprehensive monitoring of C. perfringens isolates is strongly recommended.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Hassani

Pakbin

Brück³

et al. 2022

Antibiotics

View full text Add to dashboard Cite

show abstract

“…Alongside this, spores may germinate under favourable conditions and may cause spoilage and/or produce toxins. Most important examples are Clostridium and Bacillus spp., which may contaminate foods and are often accountable for food safety and quality issues [1][2][3]. Various sources throughout the dairy chain, e.g., water, soil, dirty udders, milking equipment and faeces have been attributed to the contamination of raw milk [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterisation of Spore-Forming Bacteria in Bovine Raw Milk Collected from Four Dairy Farms in New Zealand

Gupta,

Brightwell

2023

Dairy

View full text Add to dashboard Cite

Contamination of milk and dairy products with pathogenic and spoilage bacteria may result in huge economic loss due to recalls of products. This study aimed to identify spore-forming bacteria from raw milk and characterise those for toxin production and their spoilage ability. Bovine raw milk collected from dairy farms in the Manawatu region of the North Island of New Zealand was tested for the presence of both aerobic and anaerobic spore-forming bacteria using standard culture-based techniques, as well as genomic analysis. The spore-forming bacteria were investigated for the presence of toxin genes and their spoilage potential. A low number of aerobic spore-forming bacteria were detected in raw-milk samples collected from the four farms in summer and winter. The 16S rRNA sequence types similar to important food spoilage bacteria like C. beijerinckii, C. sporogenes, B. licheniformis and members of the Paenibacillus genus, as well as potentially toxigenic bacteria such as B. cereus and C. perfringens were isolated. Genes responsible for important toxin production were present in some of the tested spore-forming bacteria. This pilot study highlights the presence of various spoilage and pathogenic spore-forming bacteria in raw milk from these farms. A low number of spore-forming bacteria indicates the implementation of good hygienic farm practices and management to reduce the contamination of raw milk with spore-forming bacteria.

show abstract

“…It has been estimated that up to 15% of all AAD patients were infected with enterotoxigenic C. perfringens 3,10 . C. perfringens has historically been classified into five toxigenic types (A-E) depending upon its ability to produce four major exotoxins including α-, β-, ɛ-, and ɩ-toxin encoded by cpa , cpb , etx , and iap/ibp genes, respectively 11,12 . However, based on a recently introduced toxin-based typing system, C. perfringens is now reclassified into seven toxinotypes (A-G) 13 .…”

Section: Introductionmentioning

confidence: 99%

Prevalence and characterization of Clostridium perfringens toxinotypes among patients with antibiotic-associated diarrhea in Iran

Azimirad

Gholami

Yadegar

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Clostridium perfringens has emerged as an important cause of antibiotic-associated diarrhea (AAD), particularly in the hospital environment. Here we investigated the prevalence and molecular epidemiology of C. perfringens isolated from 2280 fecal samples from Iranian diarrheal patients suspected of having AAD. Overall, AAD was diagnosed in 13.3% (303/2280) of patients and associated with advanced age (>50 years, P = 0.001). A total of 106 C. perfringens isolates were cultured from AAD ( n = 68) and non-AAD ( n = 38) groups, with toxinotypes A and F comprising 84% and 16% of isolates, respectively. Notably, 41.2% of type F strains were also cpb2 -positive and enterotoxigenic cpe -positive strains were detected in 13.2% of the isolates from AAD patients. Genes associated with the VirR/VirS signal transduction ( virR , virS ) and accessory gene regulator ( agrB , agrD ) systems were detected in 56.6% and 67% of the isolates, respectively, and peptides of the quorum-sensing modulator, AgrD were highly conserved across all strains. The high prevalence of C. perfringens in Iranian AAD patients suggests that diagnostic laboratories in this region should consider screening for C. perfringens in cases of suspected AAD, especially if the specimen is negative for other pathogens and/or the patients are aged >50 years.

show abstract

Genetic variation among Clostridium perfringens isolated from food and faecal specimens in Lagos

Cited by 8 publications

References 27 publications

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Prevalence, Antibiotic Resistance, Toxin-Typing and Genotyping of Clostridium perfringens in Raw Beef Meats Obtained from Qazvin City, Iran

Identification and Characterisation of Spore-Forming Bacteria in Bovine Raw Milk Collected from Four Dairy Farms in New Zealand

Prevalence and characterization of Clostridium perfringens toxinotypes among patients with antibiotic-associated diarrhea in Iran

Contact Info

Product

Resources

About