Genetic variants of TLR9 Gene and Chronic Kidney Disease Susceptibility

Abstract

Objectives Chronic kidney disease (CKD) is a common condition that can lead to renal dysfunction and is closely in relation to an increased cardiovascular risk and mortality risk. CKD is an important public health issue, and recent genetic studies have verified common CKD susceptibility variants. In this research, we examined the interrelationship between candidate genes polymorphisms of IFNL induction and signaling pathway and CKD. Methods 92 CKD patients and 330 healthy subjects as control were participated in this research. Replication set consisting 137 CKD with CGN patients and 446 controls was used for additional analysis. The genotype of SNPs was determined by the Axiom Genome-Wide Human Assay and SNaPshot assay. Results The SNPs of IFNL3 and IFNL2 were significantly associated with chronic kidney disease in the codominant (p = 0.015, p = 0.013, respectively). The SNP of IFNRA2 was significantly associated with chronic kidney disease in the codominant (p = 0.029). The SNP of TLR9 was significantly associated with chronic kidney disease in the codominant (p = 0.016), dominant (p = 0.047) and recessive (p = 0.049). The SNP of IL-22 was significantly associated with chronic kidney disease in the codominant (p = 0.049). No significant associations involving IFNL3, IFNL2 and IL22 were observed in the replication set whereas concerning the rs187084, in the TLR9 gene, a significant association was observed pooling the original and the replication sets. Conclusions This results shows a possibility that IFNL induction and signal pathway genes polymorphisms as a risk factor for CKD. Secondly, our results seem to TRL9 gene variant may be play a risk factor on CKD with CGN.