Genetic Variant Discovery and Its Use in Genome Characterization of Agronomically Important Crop Species

Deschamps, Stéphane; Campbell, Matthew A.

doi:10.1002/9781118473023.ch9

Cited by 1 publication

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“…Transcriptome sequencing has been employed in other functional genomics methodologies, viz., genome annotation, gene expression profiling, and non-coding RNA identification employed transcriptome sequencing (Morozova and Marra, 2008). In recent years, for generating a large number of transcript reads from a variety of developing and distress-responsive tissues in several leguminous crops through several low-cost sequencing systems has already been established, viz., an improved transcriptome assembly, utilizing FLX/ 454 sequencing together with Sanger ESTs comprised 103,215 Transcript Assembly Contigs (TACs) with an average contig length of 459 base pairs in chickpea (Hiremath et al, 2011) Introns, and so on in soybean and common bean 1,682 and 4,099 SNPs, respectively (Deschamps and Campbell, 2012), ESTs comprising of 103,215 Transcript Assembly Contigs (TACs) in chickpea (Hiremath et al, 2011) can be utilized by the breeders to achieve a better grasping of the molecular underpinnings of distress tolerance and as a result more stress-tolerant beans as well chickpea cultivars may be produced and narrow genetic base may be broadened.…”

Section: Transcriptomics Utilization For Broadening the Genetic Basesmentioning

confidence: 99%

Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses

Singh

Ambika

et al. 2022

Front. Genet.

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Legume crops provide significant nutrition to humans as a source of protein, omega-3 fatty acids as well as specific macro and micronutrients. Additionally, legumes improve the cropping environment by replenishing the soil nitrogen content. Chickpeas are the second most significant staple legume food crop worldwide behind dry bean which contains 17%–24% protein, 41%–51% carbohydrate, and other important essential minerals, vitamins, dietary fiber, folate, β-carotene, anti-oxidants, micronutrients (phosphorus, calcium, magnesium, iron, and zinc) as well as linoleic and oleic unsaturated fatty acids. Despite these advantages, legumes are far behind cereals in terms of genetic improvement mainly due to far less effort, the bottlenecks of the narrow genetic base, and several biotic and abiotic factors in the scenario of changing climatic conditions. Measures are now called for beyond conventional breeding practices to strategically broadening of narrow genetic base utilizing chickpea wild relatives and improvement of cultivars through advanced breeding approaches with a focus on high yield productivity, biotic and abiotic stresses including climate resilience, and enhanced nutritional values. Desirable donors having such multiple traits have been identified using core and mini core collections from the cultivated gene pool and wild relatives of Chickpea. Several methods have been developed to address cross-species fertilization obstacles and to aid in inter-specific hybridization and introgression of the target gene sequences from wild Cicer species. Additionally, recent advances in “Omics” sciences along with high-throughput and precise phenotyping tools have made it easier to identify genes that regulate traits of interest. Next-generation sequencing technologies, whole-genome sequencing, transcriptomics, and differential genes expression profiling along with a plethora of novel techniques like single nucleotide polymorphism exploiting high-density genotyping by sequencing assays, simple sequence repeat markers, diversity array technology platform, and whole-genome re-sequencing technique led to the identification and development of QTLs and high-density trait mapping of the global chickpea germplasm. These altogether have helped in broadening the narrow genetic base of chickpeas.

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Section: Transcriptomics Utilization For Broadening the Genetic Basesmentioning

confidence: 99%

Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses

Singh

Ambika

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

show abstract

Genetic Variant Discovery and Its Use in Genome Characterization of Agronomically Important Crop Species

Cited by 1 publication

References 125 publications

Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses

Exploring Chickpea Germplasm Diversity for Broadening the Genetic Base Utilizing Genomic Resourses

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