Genetic variability of wild Agave angustifolia populations based on AFLP: A basic study for conservation

Teyer, Lorenzo Felipe Sánchez; Moreno-Salazar, Sergio Francisco; Esqueda, Martín; Barraza, Aarón; Robert, Manuel L.

doi:10.1016/j.jaridenv.2009.01.008

Cited by 31 publications

(18 citation statements)

References 26 publications

(28 reference statements)

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“…glabrescens and B. repanda subsp. baldensis are indeed moderately low compared to those reported by other AFLP studies on herbaceous outcrossing and animal‐pollinated species (Gaudeul, Taberlet & Till‐Bottraud, 2000; Chung, Gelembiuk & Givnish, 2004; Kreivi et al ., 2005; Sánchez‐Teyer et al ., 2009). Despite the lack of field data concerning their mating system, AMOVA within taxa on the different sampling localities show, for both B. repanda subsp.…”

Section: Discussionmentioning

confidence: 99%

Application of the unified species concept reveals distinct lineages for disjunct endemics of the Brassica repanda (Brassicaceae) complex

Lega

Fior

Prosser

et al. 2012

Biological Journal of the Linnean Society

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Species delimitation is an important issue in terms of conservation priorities, especially for narrow endemics under threat of extinction. The Alpine endemics Brassica repanda subsp. glabrescens and subsp. baldensis belong to a highly polymorphic species complex, although their disjunct distribution suggests favourable conditions for independent evolution. In the present study, we applied the unified species concept to test whether the endemics form distinct evolutionary lineages, both from one another and also from the remainder of the complex. Compliance with the criteria of monophyly, diagnosability, and genotypic clustering was examined, primarily by making use of amplified fragment length polymorphism data. Both endemics were indicated as monophyletic by phylogenetic analyses, and diagnostic characters were found for both taxa. Population structure analyses showed clear genetic discontinuity for each of the endemics, with little admixture among the clusters. This evidence indicates that the endemics have acquired multiple properties that satisfy each of the species criteria considered. Hence, we suggest the taxonomic recognition of B. baldensis and B. glabrescens as separate species. Comparative population genetics analyses show the lack of marked genetic structuring within either taxon, as well as low levels of heterozygosity. Conclusions on the status of threat and on recommended conservation actions are drawn. © 2012 The Linnean Society of London, Biological Journal of the Linnean Society, 2012, 106, 482–497.

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Section: Discussionmentioning

confidence: 99%

Application of the unified species concept reveals distinct lineages for disjunct endemics of the Brassica repanda (Brassicaceae) complex

Lega

Fior

Prosser

et al. 2012

Biological Journal of the Linnean Society

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“…We detected differences within A. tequilana individuals and our study was conducted using a large sample (100 individuals), which contrasts with the small samples used in previous studies (Gil-Vega et al, 2001;2006). The FST values indicate genetic differentiation within A. tequilana and A. angustifolia, as reported by Sanchez-Teyer et al (2009). The model-based clustering method using multilocus genotype data based on ISTR molecular markers provided information on the population structure of A. tequilana and other Agavaceae species.…”

Section: Discussionmentioning

confidence: 51%

“…Thus, offshoots provide a certain level of agave uniformity, but different levels of genetic variability have been detected in A. tequilana (Gil-Vega et al, 2006;Torres-Moran et al, 2010) and several other Agave spp. using molecular tools (Magdub-Mendez, 2000;Demey et al, 2004;Infante et al, 2007;Sanchez-Teyer et al, 2009). Other studies have also reported variability in the morphological characteristics of the Agave genus.…”

Section: Introductionmentioning

confidence: 99%

Variability and Genetic Structure in a Commercial Field of Tequila Plants, Agave tequilana Weber (Agavaceae)

Torres-Morán¹,

Velasco-Ramírez²,

Pena³

et al. 2013

American Journal of Agricultural and Biological Sciences

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Crops of the tequila plant (Agave tequilana) are produced mainly from offshoots of mother plants in established commercial fields. This propagation method is significant, as it is believed that it facilitates the spread of disease because of the crop's low genetic variability and is also necessary because it is regulate the use of just that variety in tequila industry. Different levels of genetic variability have been reported for A. tequilana and so we tested individuals from representative cultivation zones to determine the actual variability in fields and to assess the genetic structure of populations in commercial plantations. Four additional Agave spp. were used as a control group while Fourcrea spp. individuals were used as an external group. Morphological traits and molecular markers were analyzed. The differences between A. tequilana individuals collected from southern Jalisco state and those collected in the principal Denomination of Origin zone confirmed the existence of different genotypes, which were conserved in different regions by asexual propagation. Leaf length, plant height and number of leaves were the most significant variables that explained the variability within the A. tequilana group. At the molecular level, we found genetic differentiation with a minimum similarity of 0.253 (Jaccard's coefficient) and genetic structure analysis indicated five groups with significant genotypic differences. Genetic structure analysis, grouped accessions according to the dispersion of plant material from the initial sites of cultivation. These results might facilitate the correlation of different groups with crop yield or tequila quality and the establishment of elite lines for breeding programs. It is recommendable in a future, to determinate the different levels of inulines produced by each detected group.

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“…Although there are no reports of the genetic characteristics of the species of Agave in the Dominican Republic and their molecular characterization, the reports on these issues are abundant in the scientific literature for many species of this genus that grow in several areas of the world [3,4,5,6,7,8,9,10,11,12,13].…”

Section: Agave Antillarummentioning

confidence: 99%

Molecular characterization of beast’s maguey (Agave antillarum Descourt), in the Dominican Republic

Núñez¹,

Hoda²

2018

Preprint

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Background:This research project was proposed to determine the genetic variability (if any) of beast maguey (Agave antillanarum Decourt) populations, by means of molecular markers of the AFLPs (Amplified Fragment Length Polymorphism) type. For the determination of genetic variability, samples were collected from wild populations of maguey growing in some regions of the Dominican Republic, mainly the north, northwest, south and southwest regions, using statistical techniques already established for these cases. These samples were processed and analyzed in the plant molecular biology laboratory of the Centtro de Biotecnología Vegetal (CEBIVE) of Instituto de Innovación en Biotecnología e Industria (IIBI) in Santo Domingo, Dominican Republic.Results: As for the genetic diversity is concerned, it was found that several of the populations studied differ from each other without taking into account the geographic distance where they grow. We concluded that there is a possibility that more than one species of Agave antillarun could be found in the wild in the Dominican Republic.---

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Genetic variability of wild Agave angustifolia populations based on AFLP: A basic study for conservation

Cited by 31 publications

References 26 publications

Application of the unified species concept reveals distinct lineages for disjunct endemics of the Brassica repanda (Brassicaceae) complex

Application of the unified species concept reveals distinct lineages for disjunct endemics of the Brassica repanda (Brassicaceae) complex

Variability and Genetic Structure in a Commercial Field of Tequila Plants, Agave tequilana Weber (Agavaceae)

Molecular characterization of beast’s maguey (Agave antillarum Descourt), in the Dominican Republic

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