Genetic variability of tyrosinase gene in Egyptian camel breeds and its association with udder and body measurements traits in Maghrebi camel breed

Association of <i>β</i>-casein gene polymorphism with milk composition traits of Egyptian Maghrebi camels (<i>Camelus dromedarius</i>)

“…In this study the heterozygous A/G genotype recorded the highest frequency of 50 % while the A/A and G/G genotypes occurred at frequencies of 38 % and 12 %, respectively. Similar trends were reported by Pauciullo et al (2014), who identified three CSN2 genotypes -A/G (51 %), A/A (40 %), and G/G (9 %) -across four populations of Sudanese camels (Shanbali, Kahli, Lahaoi, and Arabi) using PCR-RFLP method. Our results showed that Maghrebi camels with A/A genotypes had a higher protein percentage with higher acidity.…”

“…Our results showed that Maghrebi camels with A/A genotypes had a higher protein percentage with higher acidity. Previous studies have reported a significant association of CSN2 gene polymorphisms with higher protein and casein content in cattle (Nilsen et al, 2009;Bugeac et al, 2015;Bhat et al, 2017;Viale et al, 2017;Soyudal et al, 2018). The association between the CSN2 gene polymorphisms and milk protein percentage of our study might be attributed to the modification of the mRNA stability and transcription rates (Szymanowska et al, 2004;Kuss et al, 2005).…”

“…Genomic DNA from 68 hair samples was extracted using a Gene JET genomic DNA purification kit, following the manufacturer's protocol (Fermentas, Waltham, MA, USA). DNA fragments of 659 bp spanning from −428 bp of 5 flanking region to +231 bp of the camel CSN2 gene were amplified to detect the 2126 A/G SNP by using the previously published primers (Pauciullo et al, 2014). Each PCR mix consisted of 1.0 µM right and left primers, 0.2 mM dNTPs, and 1.25 U of Taq polymerase.…”

“…The distribution of calcium and the stability of casein micelle are reported to be influenced by the different levels of β-casein phosphorylation, so β-casein is an important component of camel milk, and it plays a vital role in the nutritional and processing properties of camel milk and its products (Amigo et al, 2000). The β-casein (CSN2) gene coding for β-casein of dromedary camels consists of 9 exons and extends over 7819 nucleotides (Pauciullo et al, 2014). In ruminants, several studies have shown the association of β-casein gene polymorphism with economic important traits such as yield and composition of milk in cattle (Huang et al, 2012;Viale et al, 2017;Soyudal et al, 2018), in buffalo (Singh et al, 2007), in sheep (Corral et al, 2010), and in goats (Cosenza et al, 2007).…”

“…In ruminants, several studies have shown the association of β-casein gene polymorphism with economic important traits such as yield and composition of milk in cattle (Huang et al, 2012;Viale et al, 2017;Soyudal et al, 2018), in buffalo (Singh et al, 2007), in sheep (Corral et al, 2010), and in goats (Cosenza et al, 2007). Previously, Pauciullo et al (2014) identified a transition g.2126 A>G in CSN2 promoter region of four Sudanese Camelus dromedarius populations. This 2126 A/G SNP is located three nucleotides downstream of the TATA box and might modify the binding affinity of RNA polymerase and change the gene expression (Lee et al, 2012).…”