Genetic variability of the S gene of hepatitis B virus: clinical and diagnostic impact

Weber, Bernard

doi:10.1016/j.jcv.2004.10.008

Cited by 184 publications

(155 citation statements)

References 89 publications

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“…Moreover, this mutation has been associated with antiviral treatment failure [27]. Since the HBV S gene is the main target for the specific antibody against HBV, these mutations may result in the escape of the virus from the neutralizing antibody response or the failure of antiviral therapy [19,28,29]. Therefore, the stop codon mutations in the S gene should be further investigated in T2DM patients with HBV infection, although the results of the present study did not show a statistical difference between diabetics and nondiabetics.…”

Section: Discussionmentioning

confidence: 99%

Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection

Zhu

Wang

Yu³

et al. 2016

J Infect Dev Ctries

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Introduction: This study aimed to further analyze the associations between type 2 diabetes mellitus (T2DM) and hepatitis B virus (HBV) infection, and to investigate the relationships between T2DM and the mutations within the HBV major hydrophilic region (MHR). Methodology: In this cross-sectional study, 3,377 persons (338 T2DM patients and 3,039 non-diabetics) were randomly selected. HBsAg detection was performed by enzyme-linked immunosorbent assay. The HBV MHR was amplified, sequenced, and analyzed by nested PCR. Results: The seroprevalence of HBsAg was 21.30% in T2DM patients (72/338), which was significantly higher than in non-diabetics (15.53%). Compared to persons without T2DM, the proportion of T2DM patients positive for HBsAg was significantly elevated in males, people > 55 years (p = 0.039), and people with a body mass index (BMI) ≥ 24 kg/m 2 . Totally, 112 genotype B and 111 genotype C HBV sequences were isolated. No significant difference in HBV genotype distribution was observed between T2DM patients and non-diabetics. Compared to genotype C HBV-infected cases in non-diabetics, the amino acid substitution rates in the MHR were significantly higher in T2DM patients (p = 0.003). Moreover, seven HBV strains with stop codon mutations within the HBV S gene were identified: three from T2DM patients (5.45%) and four from non-diabetics (2.38%). Conclusions: In China, T2DM is significantly associated with chronic HBV infections and genotype C HBV MHR mutations. Being males, > 55 years of age, and ≥ 24 kg/m 2 of BMI are the risk factors of HBV infection in T2DM patients.

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Section: Discussionmentioning

confidence: 99%

Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection

Zhu

Wang

Yu³

et al. 2016

J Infect Dev Ctries

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“…The S-gene, which is targeted in this assay, encodes the surface antigen (HBsAg). Though the clinical significance of S-gene mutants remains to be further investigated [11], the variability of this gene represents a challenge for the sensitivity of immunologic and molecular-based assays. Therefore, the magnetic bead assay was designed to specifically target a conserved region of the S-gene.…”

Section: Discussionmentioning

confidence: 99%

A multi-center clinical study comparing Sansure Magb and CAP/CTM HBV tests in the quantitative detection of HBV DNA

Tan

Dou

et al. 2016

J Infect Dev Ctries

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Introduction: As the most reliable means of diagnosing hepatitis (HBV) infection and predicting the prognosis of HBV-related chronic liver disease, the COBAS AmpliPrep/COBAS TaqMan real-time polymerase chain reaction (PCR) (CAP/CTM) assay provides a highly sensitive and accurate method for quantifying HBV DNA. However, the high cost of the COBAS reagents is prohibitive in many developing countries. Thus, we compared the Sansure magnetic bead (Magb) assay, a novel technology developed by a Chinese company, with the CAP/CTM assay. Methodology: The reproducibility and sensitivity of the Sansure Magb assay were first validated using HBV DNA reference samples. Next, the quantitative results for the two assays using 635 blood samples collected from chronic hepatitis B patients and 10 healthy controls were compared. Results: The Sansure Magb assay showed high reproducibility and was at least as sensitive and specific as the CAP/CTM assay. Among the patient samples, 407 tested positive by both methods, with 386 (94.84%) showing quantitative differences of less than 1 log unit and 21 (5.16%) showing quantitative differences of between 1 and 2 log units. The results from the assays were closely correlated. Bland-Altman plot analysis showed that only 6.6% of the data points fell outside the 95% limits of agreement, which suggests that the differences between methods are clinically acceptable. Conclusions: This study demonstrates that the Sansure Magb assay is highly sensitive and reproducible. Based on its reduced cost, the Sansure Magb assay may be more applicable than the CAP/CTM assay for HBV diagnosis in developing countries such as China.

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“…The "a" determinant is considered as the main neutralizing epitope and its common in all HBV genotypes, for this reason the anti HBs against the "a" determinant are widely protective against reinfection by HBV [29]. Changes in the conformation which can successively lead to failure of binding of neutralizing antibodies may develop due to amino acid substitutions within the" a" determinant [30].…”

Section: Hepatitis B Surface Antigen (Hbsag)mentioning

confidence: 99%

Hepatitis B Virus_Surface Gene Mutations and Their Clinical Implications

Hamadalnil¹,

Bakheit²

2017

SJMS

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Genetic variability of the S gene of hepatitis B virus: clinical and diagnostic impact

Cited by 184 publications

References 89 publications

Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection

Serological and molecular analysis on the relationships between type 2 diabetes mellitus and hepatitis B virus infection

A multi-center clinical study comparing Sansure Magb and CAP/CTM HBV tests in the quantitative detection of HBV DNA

Hepatitis B Virus_Surface Gene Mutations and Their Clinical Implications

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