Genetic variability of the phloem sap metabolite content of maize (Zea mays L.) during the kernel-filling period

Yesbergenova-Cuny, Zhazira; Dinant, Sylvie; Martin‐Magniette, Marie‐Laure; Quilleré, Isabelle; Armengaud, Patrick; Monfalet, Priscilla; Lea, Peter J.; Hirel, Bertrand

doi:10.1016/j.plantsci.2016.08.007

Cited by 27 publications

(28 citation statements)

References 64 publications

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“…Nevertheless, it is likely that at least in line FV252, the small decrease in the amount of root carbohydrates such as glucose and of organic acids such as trans -aconitate (the most abundant in maize [77,79]), corresponds to the utilization of the C skeletons necessary for the synthesis of larger amounts of amino acids. This utilization could also be balanced by a higher production of other organic acids such as isocitrate.…”

Section: Discussionmentioning

confidence: 99%

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

et al. 2017

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Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.

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Section: Discussionmentioning

confidence: 99%

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

et al. 2017

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“…For maize and wheat, we followed the method described by Yesbergenova-Cuny et al (Yesbergenova-Cuny et al, 2016). Leaves were cut off at their base, close to the stem, and recut under the exudation buffer (10 mM HEPES, 10 mM EDTA, adjusted to pH 7.8 with NaOH).…”

Section: Measurement Of Photosynthesis Leaf Sugar Levels and Sugar mentioning

confidence: 99%

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

et al. 2017

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Suc transporters (SUTs) play a key role in the allocation and partitioning of photosynthetically fixed carbon in plants. While a function could be assigned to many members of the SUT family, almost no information is available on their regulation. Here, the transcriptional regulation of SUTs in response to various environmental stimuli in the leaves of five dicots (Arabidopsis Extensive data on expression of SUTs in relation to changes of environmental conditions were obtained through a global analysis of 168 transcriptomics data sets. Results were validated by quantitative PCR measurements and extended by the measurement of photosynthesis rate and phloem sugar content to draw insight on the correlation of SUT expression and sugar export from leaves. For the apoplasmic phloem loaders, a clear difference in transcriptional regulation in response to different environmental stimuli was observed. The consistent patterns of SUT expression under abiotic stress indicates which types of SUTs are involved in the regulation of leaf sugar status and in stress signaling. Furthermore, it is shown that down-regulation of phloem loading is likely to be caused by transcriptional regulation of SUTs, while up-regulation depends on post-transcriptional regulation. In poplar, expression of PtaSUT4 was found to consistently respond to environmental stimuli, suggesting a significant role in the regulation of sugar export from leaves in this passive symplasmic phloem loader.

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“…Further examinations have begun to characterize the breadth of diversity of various inbreds and hybrids across typical agronomic environments particularly emphasizing these effectors on grain and forage composition (Asiago, Hazebroek, Harp, & Zhong, 2012;Baniasadi, Vlahakis, Hazebroek, Zhong, & Asiago, 2014;Benevenuto et al, 2017;Hall et al, 2016;Tang et al, 2017;Venkatesh et al, 2016). There have also been several "omic" analyses to understand the relationship of transcripts and metabolites to critical phenotypic traits including: yield (Asiago et al, 2012;Hazebroek, Janni, & Lightner, 2012;Wang, Xue, & Wang, 2014;Xu, Xu, & Xu, 2017), dry matter accumulation (Westhues et al, 2017), and silking (Yesbergenova-Cuny et al, 2016). However, it is resource intensive to collect data for a suite of traits in a consistent manner across diverse germplasm to understand the environmental and genetic control of traits.…”

Section: Introductionmentioning

confidence: 99%

Evaluating maize phenotypic variance, heritability, and yield relationships at multiple biological scales across agronomically relevant environments

Tucker

Dohleman

Flagel

et al. 2020

Plant Cell & Environment

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A challenge to improve an integrative phenotype, like yield, is the interaction between the broad range of possible molecular and physiological traits that contribute to yield and the multitude of potential environmental conditions in which they are expressed. This study collected data on 31 phenotypic traits, 83 annotated metabolites, and nearly 22,000 transcripts from a set of 57 diverse, commercially relevant maize hybrids across three years in central U.S. Corn Belt environments. Although variability in characteristics created a complex picture of how traits interact produce yield, phenotypic traits and gene expression were more consistent across environments, while metabolite levels showed low repeatability. Phenology traits, such as green leaf number and grain moisture and whole plant nitrogen content showed the most consistent correlation with yield. A machine learning predictive analysis of phenotypic traits revealed that ear traits, phenology, and root traits were most important to predicting yield. Analysis suggested little correlation between biomass traits and yield, suggesting there is more of a sink limitation to yield under the conditions studied here. This work suggests that continued improvement of maize yields requires a strong understanding of baseline variation of plant characteristics across commercially‐relevant germplasm to drive strategies for consistently improving yield.

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Genetic variability of the phloem sap metabolite content of maize (Zea mays L.) during the kernel-filling period

Cited by 27 publications

References 64 publications

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

Regulation of Sucrose Transporters and Phloem Loading in Response to Environmental Cues

Evaluating maize phenotypic variance, heritability, and yield relationships at multiple biological scales across agronomically relevant environments

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