Genetic variability of respiratory syncytial virus A in hospitalized children in the last five consecutive winter seasons in Central Spain

Calderón, Ana Belén Mayo; Pozo, Francisco; Calvo, Cristina; García-García, M.L.; González-Esguevillas, Mónica; Molinero, Mar; Casas, Inmaculada

doi:10.1002/jmv.24703

Cited by 10 publications

(11 citation statements)

References 47 publications

(98 reference statements)

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“…In our study, however, RSV-B strongly dominated the seasons 2013/14 and 2015/16. This finding suggests that the emergence of genotype ON1 has not had a noticeable effect on the overall epidemiological pattern of frequent shifts between RSV-A and RSV-B group dominance [2, 3], confirming similar observations reported from Spain [38] and Argentina [43].…”

Section: Discussionsupporting

confidence: 88%

“…During the first and the second season of RSV-A ON1 appearance in Germany, the proportion of RSV-A in our study population was considerably higher than the proportion of RSV-B, similar to reports from some other countries [19, 38, 43, 44]. It has been speculated that the rapid spread of RSV-A ON1 might also reduce RSV-B circulation in subsequent seasons [43, 44].…”

Section: Discussionsupporting

confidence: 87%

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Spread and clinical severity of respiratory syncytial virus A genotype ON1 in Germany, 2011–2017

et al. 2019

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Background The Respiratory Syncytial Virus (RSV) A genotype ON1, which was first detected in Ontario (Canada) in 2010/11, appeared in Germany in 2011/12. Preliminary observations suggested a higher clinical severity in children infected with this new genotype. We investigated spread and disease severity of RSV-A ON1 in pediatric in- and outpatient settings. Methods During 2010/11 to 2016/17, clinical characteristics and respiratory samples from children with acute respiratory tract infections (RTI) were obtained from ongoing surveillance studies in 33 pediatric practices (PP), one pediatric hospital ward (PW) and 23 pediatric intensive care units (PICU) in Germany. RSV was detected in the respiratory samples by PCR; genotypes were identified by sequencing. Within each setting, clinical severity markers were compared between RSV-A ON1 and RSV-A non-ON1 genotypes. Results A total of 603 children with RSV-RTI were included (132 children in PP, 288 in PW, and 183 in PICU). Of these children, 341 (56.6%) were infected with RSV-A, 235 (39.0%) with RSV-B, and one child (0.2%) with both RSV-A and RSV-B; in 26 (4.3%) children, the subtype could not be identified. In the 341 RSV-A positive samples, genotype ON1 was detected in 247 (72.4%), NA1 in 92 (26.9%), and GA5 in 2 children (0.6%). RSV-A ON1, rarely observed in 2011/12, was the predominant RSV-A genotype in all settings by 2012/13 and remained predominant until 2016/17. Children in PP or PW infected with RSV-A ON1 did not show a more severe clinical course of disease compared with RSV-A non-ON1 infections. In the PICU group, hospital stay was one day longer (median 8 days, inter-quartile range (IQR) 7–12 vs. 7 days, IQR 5–9; p = 0.02) and duration of oxygen treatment two days longer (median 6 days, IQR 4–9 vs. 4 days, IQR 2–6; p = 0.03) for children infected with RSV-A ON1. Conclusions In children, RSV-A ON1 largely replaced RSV-A non-ON1 genotypes within two seasons and remained the predominant RSV-A genotype in Germany during subsequent seasons. A higher clinical severity of RSV-A ON1 was observed within the group of children receiving PICU treatment, whereas in other settings clinical severity of RSV-A ON1 and non-ON1 genotypes was largely similar. Electronic supplementary material The online version of this article (10.1186/s12879-019-4266-y) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 87%

Spread and clinical severity of respiratory syncytial virus A genotype ON1 in Germany, 2011–2017

et al. 2019

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“…Group RSV-A was predominant with 64% among positive RSV cases. Similar predominance of RSV-A over RSV-B was reported earlier in Saudi Arabia, Germany, Thailand, South Africa, Spain, USA, France, Cyprus and Kuwait [6,8,[31][32][33][34][35][36][37].…”

Section: Discussionsupporting

confidence: 82%

Dominance of the ON1 Genotype of RSV-A and BA9 Genotype of RSV-B in Respiratory Cases from Jeddah, Saudi Arabia

Al-Sharif

El‐Kafrawy

Yousef

et al. 2020

Genes

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Human respiratory syncytial virus (HRSV) is a main cause of hospital admission for lower respiratory tract infection. In previous studies from Saudi Arabia, higher prevalence of the NA1 genotype in group A was observed from Riyadh and Taif. This study recruited respiratory cases from Jeddah during January to December, 2017. RSV represented 13.4% in the recruited cases with 64% of them belonging to group A and 36% to group B. All group A cases in this study were ON1 type characterized by duplication of 72 nucleotides, 24 amino acids in the C-terminal in the second hypervariable region of the G gene. In addition, for group B all of the cases were clustered under BA9, which had uniquely characterized as duplication of 60 nucleotides in the G protein. Our sequences showed similarity with earlier sequences from Saudi Arabia, Kuwait, Thailand, South Africa, Spain, the USA and Cyprus. Some amino acid substitutions in the investigated sequences would cause a change in potential O-glycosylation and N-glycosylation profiles from prototype ON1. The predominance of the ON1 and BA9 genotype of RSV-A in Jeddah compared to previous Saudi studies showing predominance of the NA1 genotype for group A. This difference in genotype prevalence could be due to fast spread of the ON1 genotype worldwide or due to the flux of travelers through Jeddah during hajj/umrah compared to Riyadh and Taif. This shift in genotype distribution requires continuous surveillance for genetic characterization of circulating respiratory infections including RSV. These findings may contribute to the understanding of RSV evolution and to the potential development of a vaccine against RSV.

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“…A report from Brazil also noted an increase of intensive care unit admissions and need for mechanical ventilation associated with ON1genotype [ 79 ]. In contrast, reports from Italy, the Philippines, South Africa, Spain found no evidence of clinical differences in disease severity in correlation to genotype [ 30 , 56 , 80 , 81 ].…”

Section: Discussionmentioning

confidence: 88%

“…However longer sequences (e.g. full lengths of G protein) are useful to elaborate more phylogenetic signal and better resolution in the evolutionary analysis [ 7 , 17 , 27 , 33 , 51 , 81 , 82 ] to understand the effect of any genetic changes on fitness, virulence and transmissibility [ 22 ]. We intend to continue investigating country wide patterns and genetic variety of HRSV and its correlation with clinical severity.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of human respiratory syncytial virus among children in Japan during three seasons and hospitalization risk of genotype ON1

et al. 2018

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We investigated the genetic diversity, the circulation patterns, and risk for hospital admission of human respiratory syncytial virus (HRSV) strains in Japan between 2012 through 2015. During the study period, 744 HRSV-positive cases were identified by rapid diagnostic test. Of these, 572 samples were positive by real-time PCR; 400 (69.9%) were HRSV-A, and 172 (30.1%) were HRSV-B. HRSV-A and -B alternated as the dominant strain in the subsequent seasons. Phylogenetic tree analysis of the second hyper-variable region of the G protein classified the HRSV-A specimens into NA1 (n = 242) and ON1 (n = 114) genotypes and the HRSV-B specimens into BA9 (n = 60), and BA10 (n = 27). The ON1 genotype, containing a 72-nucleotide duplication in the G protein’s second hyper-variable region, was first detected in the 2012–2013 season but it predominated and replaced the older NA1 HRSV-A in the 2014–2015 season, which also coincided with a record number of HRSV cases reported to the National Infectious Disease Surveillance in Japan. The risk of hospitalization was 6.9 times higher for the ON1 genotype compared to NA1. In conclusion, our data showed that the emergence and predominance of the relatively new ON1 genotype in Japan was associated with a record high number of cases and increased risk for hospitalization.

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Genetic variability of respiratory syncytial virus A in hospitalized children in the last five consecutive winter seasons in Central Spain

Cited by 10 publications

References 47 publications

Spread and clinical severity of respiratory syncytial virus A genotype ON1 in Germany, 2011–2017

Spread and clinical severity of respiratory syncytial virus A genotype ON1 in Germany, 2011–2017

Dominance of the ON1 Genotype of RSV-A and BA9 Genotype of RSV-B in Respiratory Cases from Jeddah, Saudi Arabia

Molecular epidemiology of human respiratory syncytial virus among children in Japan during three seasons and hospitalization risk of genotype ON1

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