Genetic variability of<i>Haemonchus contortus</i>(Nematoda: Trichostrongyloidea) in alpine ruminant host species

Cerutti, M.C.; Citterio, Carlo Vittorio; Bazzocchi, Chiara; Epis, Sara; D’Amelio, Stefano; Ferrari, Nicola; Lanfranchi, P.

doi:10.1017/s0022149x09990587

Cited by 67 publications

(65 citation statements)

References 48 publications

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“…Absence of host specific clustering of ND4 and COI haplotypes indicates a high rate of gene flow occurring across Haemonchus populations infecting different host species, which are maintained and grazed together under the prevailing small holder production systems in Pakistan. This feature has been broadly observed as characteristic of generalist trichostrongylid nematodes when assessed for genetic structure among isolates from sympatric (Archie and Ezenwa, 2011) as well as allopatric hosts (Webster et al, 2007;Cerutti et al, 2009). It appears that host ecology is insufficient to create significant barriers to parasite migration between host species.…”

Section: Discussionmentioning

confidence: 98%

Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Hussain

Kathiravan

Nadeem

et al. 2014

Veterinary Parasitology

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Section: Discussionmentioning

confidence: 98%

Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Hussain

Kathiravan

Nadeem

et al. 2014

Veterinary Parasitology

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“…Recent studies have focused on the distribution of the genetic variability and population structure of trichostrongylids, based on neutral markers (BLOUIN et al, 1995;BRAISHER et al, 2004;CERUTTI et al, 2010;ARCHIE;EZENWA, 2011) and genes under ATH selective pressure (SILVESTRE et al, 2009;BRASIL et al, 2012). However, data on the distribution and variation of anthelmintic resistance-conferring polymorphisms among individual animal nematode communities within herds are still scarce, especially with regard to important domestic ruminant species such as buffaloes.…”

Section: Resultsmentioning

confidence: 99%

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Nunes

Santos

Bastianetto

et al. 2013

Rev. Bras. Parasitol. Vet.

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Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the β-tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide information about the benzimidazole resistance status of the entire herd, which would enable a considerable reduction in the costs of diagnosis for the producer. Molecular diagnosis has practical advantages, since it can guide the choice of anthelmintic drug that will be used, before its application in the herd, thus reducing the economic losses driven by anthelmintic resistance.Keywords: Anthelmintic resistance, benzimidazole drugs, molecular diagnosis, domestic ruminants, genetic polymorphisms. ResumoA resistência aos anti-helmínticos é um problema crescente que ameaça a produção pecuária em todo o mundo. A compreensão da base genética da resistência ao benzimidazol permitiu, recentemente, o desenvolvimento de métodos diagnósticos moleculares promissores. Neste estudo, isolados de Haemonchus contortus obtidos a partir de rebanhos de caprinos, ovinos e bubalinos criados no Brasil foram avaliados quanto à presença do polimorfismo F200Y no gene da β-tubulina1, o qual confere resistência ao benzimidazol. A frequência alélica da mutação variou de 7% a 43%, indicando que a resistência ao benzimidazol pode ser encontrada em nematoides isolados a partir de todas as espécies de ruminantes pesquisadas. Embora tenha sido observada variação significativa das frequências de mutação F200Y entre rebanhos/espécies hospedeiros distintos, não foi encontrada variação significativa entre populações isoladas de animais dentro de um mesmo rebanho. Estes achados sugerem que a avaliação de amostras de alguns poucos animais tem o potencial de fornecer informações sobre o nível de resistência ao benzimidazol de todo o rebanho, possibilitando uma redução considerável dos custos de diagnóstico para o produtor. O diagnóstico molecular apresenta vantagens práticas, uma vez que pode guiar a escolha da base anti-helmíntica a ser utilizada antes da sua aplicação no rebanho, reduzindo, portanto, as perdas ocasionadas pela resistência aos fármacos anti-helmínticos.Palavras-chave: Resistência a anti-helmínticos, benzimidazóis, diagnóstico molecular, ruminantes domés...

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“…In recent years, mitochondrial DNA (mtDNA) sequences have been widely used as useful genetic markers for the identification and phylogenetic studies of different parasite groups (Gasser and Newton 2000;Blouin 2002;Li et al 2008;Zhao et al 2009;Cerutti et al 2010;Dai et al 2012;Lin et al 2012;Liu et al 2012a,b;Wang et al 2012). mtDNA sequences also provide valuable markers for genetic studies of trematodes.…”

Section: Introductionmentioning

confidence: 99%

Characterization ofDicrocoelium chinensisfrom domestic yaks in Gansu and Sichuan provinces, China, using genetic markers in two mitochondrial genes

Wang¹,

Zhao²,

Li³

et al. 2012

Mitochondrial DNA

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Dicrocoelium chinensis is commonly known as the "lancet fluke" or "small liver fluke" which lives in the bile ducts and gall bladder of domestic and wild ruminants. In this study, we examined sequence variability in two mitochondrial (mt) genes, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes, among D. chinensis isolated from domestic yaks in Gansu and Sichuan provinces, China. A portion of the cox1 (pcox1) and nad1 (pnad1) genes was amplified by PCR separately from the individual adult D. chinensis, and the amplicons were subjected to sequencing from both directions. The lengths of the sequences of pcox1 and pnad1 were 358 and 458 bp, respectively. The A+T contents of the sequences were 58.94-59.22% for pcox1 and 60.04-60.48% for pnad1, respectively. The intra-specific sequence variations within D. chinensis were 0-0.84% for pcox1 and 0-0.87% for pnad1. The inter-specific sequence differences among members of the examined trematodes were significantly higher, being 22.9-29.3% and 33.6-41.5% for pcox1 and pnad1, respectively. Phylogenetic analysis utilizing combined sequences of pcox1 and pnad1 using Bayesian inference revealed that all of the D. chinensis isolates grouped together with high statistical support. These findings demonstrated the existence of low intra-specific variation in mitochondrial DNA (mtDNA) sequences among the D. chinensis isolates from domestic yaks in China and had implications for studying the molecular epidemiology and population genetics of D. chinensis.

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Genetic variability ofHaemonchus contortus(Nematoda: Trichostrongyloidea) in alpine ruminant host species

Cited by 67 publications

References 48 publications

Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Sympatric species distribution, genetic diversity and population structure of Haemonchus isolates from domestic ruminants in Pakistan

Frequency of benzimidazole resistance inHaemonchus contortus populations isolated from buffalo, goat and sheep herds

Characterization ofDicrocoelium chinensisfrom domestic yaks in Gansu and Sichuan provinces, China, using genetic markers in two mitochondrial genes

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