Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Ramesha, K. P.; Rao, A. Narasimha; Basavaraju, M.; Geetha, GR; Kataktalware, M. A.; Jeyakumar, Sakthivel

doi:10.4314/sajas.v45i5.5

Cited by 18 publications

(17 citation statements)

References 27 publications

(32 reference statements)

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“…GH is an anabolic hormone synthesized and secreted by the somatotroph cells, it is a major regulator of postnatal growth and metabolism in mammals, and it plays an important role in lactation, protein, lipid and carbohydrate metabolism, tissue growth, and fertility in cows (Akers, 2006;Lucy, 2008;Thidar et al, 2008). The growth promoting and metabolic actions of GH are generally mediated by the insulin-like growth factor I (IGF-I) (Ramesha et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

et al. 2018

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Abstract. Research to assess the effect of single genes on reproductive traits in bovine species is imperative to elucidate genes' functions and acquire a better perspective of quantitative traits. The present study was undertaken to characterize genetic diversity in the bovine growth hormone (GH) gene in a population of 410 Holstein dairy cows in Tunisia. The analyses were based on single nucleotide polymorphisms, and GH-AluI and GH-MspI detections and genotyping were carried out using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Data were analyzed using a mixed linear model with the MIXED procedure to reveal the possible effect of GH genotypes on reproductive traits. The frequency data of AluI(L//V) and MspI(+//−) alleles were 87.04//12.96 and 70.06//29.94, respectively. The distribution of the frequency of GH genotypes for LL/LV/VV and (−/−)//(+/−)//(+/+) were 77.75//18.59//3.66 and 15.37//29.13//55.50, respectively. The results of the statistical analyses proved that GH-AluI showed a substantial favorable effect on exanimate traits except for the age at first calving; however, only a suggestive effect of GH-MspI on the calving interval (CI) and the days open (DI) was found. The homozygous LL genotype seemed to be advantageous with respect to the CI and the DI compared with LV and VV genotypes. Heterozygous MspI(+/−) cows tended to have a longer CI and DI than MspI(+/+) and MspI(−/−) cows, but the difference was not statistically significant. A significant effect of different GH-AluI–MspI combined genotypes was found on the number of inseminations per conception, the CI and the DI, and the LL/– combined genotype seemed to be associated with better reproductive performance. Based on these results, the LL genotype of the GH locus can be considered to be a favorable genotype for reproductive traits in Holstein dairy cattle, although these findings need to be confirmed by further research before polymorphisms can be used in a marker-assisted selection program.

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Section: Introductionmentioning

confidence: 99%

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

et al. 2018

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“…According to Othman et al (2014), IGFBP-3 can be used as a marker for the function of growth, metabolism, reproduction, immunity and energy balance in cattle. SNP is a condition where the presence of a different nucleotide constituent of a gene, and studies of SNP on IGFBP-3 gene in sheep, goat, cattle and buffalo have been reported (Rodríguez et al 2013;Mahrous et al 2014;Ramesha et al 2015;Rasouli et al 2016). SNP in the IGFBP-3 gene was reported in Egypt cattle (Othman et al 2014), Holstein and Cross between Holstein-Haryana (Choudhary et al 2007), Hanwoo (Kim et al 2005) and various Chinese cattle (Gao et al 2009;Liu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

et al. 2017

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Abstract. Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functions that influence the growth, energy metabolism, reproduction and immunity. The genome was isolated from whole blood samples of 10 Brahman cattle and 16 Brahman Cross cattle. Cattle IGFBP-3 gene that targeted in this study was located in the part of intron 2, exon 3 and part of intron 3. The gene targets were amplified using specific primers by Polymerase Chain Reaction (PCR) technique, resulting 563 bp amplification product. As data comparison to reveal the SNP, the GenBank sequences (n = 14) from various breed and countries were used. The objective of this study was to reveal the SNP on the Brahman and Brahman Cross IGFBP-3 gene. The results obtained 3 genotypes from one SNP that spread in the sample population. The SNP was located in intron 2 at position 3,930 (GA). The Polymorphism could be recognized by PvuII restriction enzymes. There was not enough evidence to associate the SNP with the phenotype (at pre-weaning age) of Brahman and Brahman Cross cattle. There was a genetic diversity in the population studied. Knowledgeable SNP could be used as genetic markers for further research on Brahman and Brahman Cross cattle.

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“…GH and GHR were previously reported as particular gene regions for meat yield in farm animals, and they were selected as candidate genes for molecular selection (Biswas et al, 2003;Andreas et al, 2010). Specific primer pairs that were previously described for cattle (Maciulla et al, 1997) were used for these two gene regions in water buffaloes, and PCR products were obtained with similar fragment lengths from water buffalo samples (Andreas et al, 2010;Ramesha et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Investigation of GH and GHRAlu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

Özşensoy¹,

Kara²

2019

Turk J Zool

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The Anatolian water buffalo is a native water buffalo breed only being reared in Turkey. The objective of this study was to investigate gene polymorphisms on exons 4 and 5 of the growth hormone (GH) and on exon 10 of the growth hormone receptor (GHR), which are thought to be related to meat yields of Anatolian water buffalo. Blood samples of 192 water buffaloes from Sivas Province were used in DNA extractions by phenol-chloroform method. DNA samples were amplified by using specific primers in PCR. PCR products were digested by Alu I (GH, n: 167) and Alu I (GHR, n: 192) restriction enzymes in order to determine polymorphisms. Digested PCR products were separated in 2.5%-3% agarose gel electrophoresis to determine allelic polymorphisms. As a result, the LL (78.44%) and LV (21.56%) genotypes and L (0.89) and V (0.11) alleles for the GH gene and the AA (7.81%) and AG (92.19%) genotypes and A (0.54) and G (0.46) alleles for the GHR gene were obtained. Gene polymorphisms were not detected (P > 0.05) on the GH gene, but a significant difference was found for the GHR gene (P < 0.001). Therefore, it can be said that Anatolian water buffaloes have increased meat yield due to the presence of genotypes in gene regions. This was the first investigation of enzyme polymorphisms of the GHR gene in Anatolian water buffaloes.

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Genetic variability of bovine GHR, IGF-1 and IGFBP-3 genes in Indian cattle and buffalo

Cited by 18 publications

References 27 publications

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

Assessment of growth hormone gene polymorphism effects on reproductive traits in Holstein dairy cattle in Tunisia

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

Investigation of GH and GHRAlu I gene polymorphisms on meat yields in Anatolian water buffalo breed using PCR-RFLP method

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