Genetic variability in regenerated plants of Ungernia victoris

Bublyk, O. M.; Андреев, И. О.; Spiridonova, K. V.; Кунах, В. А.

doi:10.1007/s10535-012-0106-2

Cited by 12 publications

(7 citation statements)

References 24 publications

(23 reference statements)

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“…These results are consistent with the data of other studies (Guo et al 2006;Bairu et al 2011;Bublyk et al 2012). In particular, it is known that regenerants derived through direct organogenesis exhibit less genetic variation than plants regenerated via tissue culture (Kunakh 2005).…”

Section: Dependence On the Mode Of Regenerationsupporting

confidence: 94%

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Кунах

Mel’nyk

Дробык

et al. 2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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This chapter presents the results of cytogenetic and molecular genetic studies on tissue and organ cultures from some Gentiana species. Cytogenetic analysis of calli from seven gentians showed that all of them (except for G. punctata) consisted mainly of the cells with diploid and/or near-diploid chromosome sets with low anaphase aberration. Species specificity of cytogenetic structure was established for cell populations in vitro. An increase in the proportion of polyploid cells was found for G. acaulis callus after long-term culture. RAPD analysis of tissue cultures from six gentians showed that culture resulted in genetic changes, with the species differing in the level of genetic variation. RAPD and ISSR analyses of G. pneumonanthe and G. lutea genetic variation in tissue and organ cultures and regenerated plants revealed the most significant changes in isolated root cultures as well as in long-term cultured tissues. At the same time, direct and indirect regenerants had the least genetic difference compared to donor plant. The restriction maps of nuclear 18S-25S ribosomal DNA from G. acaulis, G. punctata, G. lutea, and G. asclepiadea were constructed based on the results of blot hybridization. The decrease of 18S-25S rDNA amount was found in cultured tissues. In contrast to other species, G. lutea showed intragenome heterogeneity of rRNA genes as well as qualitative rDNA changes in tissue culture, such as the appearance of repeats with altered length. A relationship has been suggested between the peculiarities of the structural organization of the 18S-25S rRNA gene cluster and its rearrangements. Rearrangements of 5S rDNA were not found in cultures of Gentiana L. species, except for the variation in nucleotide methylation pattern at restriction sites.

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Section: Dependence On the Mode Of Regenerationsupporting

confidence: 94%

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Кунах

Mel’nyk

Дробык

et al. 2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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“…These results are consistent with the data of other studies Bairu et al 2011;Bublyk et al 2012). In particular, it is known that regenerants derived through direct organogenesis exhibit less genetic variation than plants regenerated via tissue culture .…”

Section: Dependence On the Mode Of Regenerationsupporting

confidence: 94%

The Gentianaceae - Volume 2: Biotechnology and Applications

Rybczyński¹,

Davey²,

Mikuła³

2015

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“…In spite of this limitation, the MSAP method has been successfully applied in a wide range of studies where alterations in cytosine methylation were detected in various crop species (Bednarek et al 2007). The AFLP and the MSAP markers have been used to detect significant genetic and epigenetic changes, respectively in a number of crop species for example potato (Joyce and Cassells, 2002;Dann and Wilson, 2011), Solanum aculeatissimum (Ghimire et al 2012), Triticum aestivum (Meng et al 2012), Gardenia jasminoides (Wu et al 2012), Ungernia victoris (Bublyk et al 2012), Capparis spinosa (Carra et al 2012), Phaseolus ssp. (Abid et al 2011), Nicotiana tabacum (Yang et al 2011), Freesia hybrida (Gao et al 2010), Ocotea catharinensis (Hanai et al 2010), Cymbidium (Chen et al 2009), Vitis spp.…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

Tiwari¹,

Saurabh²,

Chandel³

et al. 2013

Electron. J. Biotechnol.

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Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1 st cycle) and their regenerants (30 th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band in their regenerants. AFLP profiles and their cluster analysis based on the Jaccard's similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. On the other hand, to analyze epigenetic changes, eight MSAP primer pair combinations detected a few DNA methylation patterns in the mother plants (0 to 3.4%) and their regenerants (3.2 to 8.5%). Out of total 2320 MSAP sites in the mother plants, 2287 (98.6%) unmethylated, 21 (0.9%) fully methylated and 12 (0.5%) hemimethylated, and out of total 2494 MSAP sites in their regenerants, 2357 (94.5%) unmethylated, 79 (3.1%) fully methylated and 58 (2.3%) hemi-methylated sites were amplified. Conclusion: The study concluded that no genetic variations were observed among the somatic hybrids mother plants and their regenerants by eight AFLP markers. However, minimum epigenetic variations among the samples were detected ranged from 0 to 3.4% (mother plants) and 3.2 to 8.5% (regenerants) during the tissue culture process.

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Genetic variability in regenerated plants of Ungernia victoris

Cited by 12 publications

References 24 publications

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

Genetic Variation Induced by Tissue and Organ Culture in Gentiana Species

The Gentianaceae - Volume 2: Biotechnology and Applications

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

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