Genetic variability in populations of Agave sisalana perrine detected by inter simple sequence repeats

Santos, Silvia Letícia Bispo dos; Passos, Adriana Rodrigues; Queiroz, Sandra Regina de Oliveira Domingos; Nascimento, Marilza Neves do; Carneiro, Fernando dos Santos

doi:10.14393/bj-v31n6a2015-26420

Cited by 3 publications

(2 citation statements)

References 22 publications

(14 reference statements)

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“…Most molecular markers are robust to identify variability in sexually or asexually propagated populations. In literature, ISSR, amplified fragment length polymorphism (AFLP) and microsatellites markers are the most reported (Barraza-Morales et al, 2006;Abraham-Juárez et al, 2009;Santos et al, 2015). In Agave, the production is supported by intensive clonal propagation and the suppression of the sexual reproduction, leading to reduction in genetic variability plantations (Gil-Vega et al, 2001;Infante et al, 2006;Abraham-Juárez et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

Souza¹,

Cavalcanti²,

Ramos³

et al. 2018

Afr. J. Agric. Res.

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The Agave genus is composed of about 200 species, but the cultivation of sisal for fiber production in Brazil is restricted to two species: Agave sisalana and Agave fourcroydes, both have several accessions with wide variability. The collection of Agave of Embrapa has 37 accessions maintained in situ and periodically evaluated agronomical traits. Most of these accessions have phenotypic similarities, although they differ in fiber quality, which are widely used for commercial purposes. The identification of promising accesses contributes to the advance in improvement works, focusing on commercial indication. In order to estimate the genetic divergence of this collection, a cluster analyses was performed based on Inter-Simple Sequence Repeat (ISSR) markers and phenotypic traits. Genomic DNA from these accessions were used in polymerase chain reaction (PCR) with thirty ISSR oligonucleotides. For phenotypic characterization, twelve descriptors were adopted based on morphological and agronomic data. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) and Tocher multivariate methods were adopted. Thirteen groups were formed by the Tocher Method and six by UPGMA; however UPGMA method was more representative in the group formation. The comparison of the band patterns among accessions derived from the shoots showed that genetic variability is generated during asexual reproduction in these plants. The four lines generated from Tatui were the most divergent accessions. These plants are tallest, with higher mass values of fresh and dry mucilage, fresh and dry fiber mass, fiber length and presence of spines at the edges. The accessions from Instituto Agronômico de Campinas (IAC) showed the lowest genetic distances, indicating a possible narrow genetic base and high kinship degree. The crossings between H-RN, H-Kenya, H-400 fls, and H-11648 with Tatuí 1, 2, 3, and 4 can be a valorous strategy to broadening genetic diversity among commercial and native sisal germplasm.

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Section: Resultsmentioning

confidence: 99%

Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

Souza¹,

Cavalcanti²,

Ramos³

et al. 2018

Afr. J. Agric. Res.

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“…In this study, genetic polymorphism in the populations was evaluated through the use of molecular markers that have the advantage of not being influenced by environmental factors (Laakili et al 2018). Dominant markers, such as ISSRs, allow rapid, simple, and reproducible analysis and exhibit sufficient polymorphism to assess genetic diversity or identify closely related cultivars in many species, including fruit trees (Santos et al 2015;Paschoa et al 2018). Therefore, we used an ISSR approach to evaluate the diversity and genetic structure of cajui from three natural populations in PNSC and two accessions from Pacajus (Caju I and Caju-II).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and structure in natural populations of Cajui from Brazilian Cerrado

Gomes

Borges²,

Batista

et al. 2021

Biosci. J.

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Cajui (Anacardium spp.) is a native fruit tree (small cashew) of the Brazilian Cerrado and possesses the potential for commercialization. However, cajui exploitation occurs exclusively through extractivism in the absence of conservation strategies. The lack of conservation strategies may lead to a decrease in genetic diversity of Anacardium. In this work, the genetic diversity and population structure of three natural populations in Sete Cidades National Park (PNSC; PI, Brazil) were assessed using ISSR analysis of 56 cajui accessions and two A. occidentale accessions (outgroup) from Pacajus (CE, Brazil). A total of 112 markers were obtained, 93 (83.04%) of which were polymorphic. The diversity indices of these populations indicated moderate levels of genetic diversity. According to AMOVA, 96.17% of the genetic variability lay within populations, with low genetic differentiation among populations (ΦST = 0.03828). Furthermore, STRUCTURE analysis indicated the existence of four connected genetic groups. The findings show that the individuals from the three collection sites did not represent different subpopulations, likely due to the high gene flow (Nm = 6.7) favored by the floral biology of Anacardium, pollinators and small-animal seed dispersers. This research identifies genetically divergent individuals (C-03, C-05, C-22, C-26, C-34 and C-39), which should be considered priority individuals for conservation and can inform conservation programs for Anacardium spp.

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Breeding Strategies to Improve Production of Agave (Agave spp.)

Monja-Mio

Herrera-Alamillo

Teyer

et al. 2019

Advances in Plant Breeding Strategies: Industrial and Food Crops

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Genetic variability in populations of Agave sisalana perrine detected by inter simple sequence repeats

Cited by 3 publications

References 22 publications

Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

Genetic divergence in Agave accessions through ISSR markers and phenotypic traits

Genetic diversity and structure in natural populations of Cajui from Brazilian Cerrado

Breeding Strategies to Improve Production of Agave (Agave spp.)

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