Genetic variability in mitochondrial DNA of the screwworm,Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil

Vargas, Maria Elena Infante; Azeredo-Espin, Ana Maria Lima de

doi:10.1007/bf00553622

Cited by 14 publications

(4 citation statements)

References 28 publications

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“…Total genomic DNA was extracted from legs or thoracic muscle of dried and ethanol-preserved specimens using an adapted protocol for DNAzol ® (Invitrogen) 82 or with Spin Tissue Mini-Kit (Invitek), following manufacturer’s instructions. Frozen specimens had DNA extracted with Phenol/chlorophorm 83 or using the DNeasy Blood and Tissue Kit (Qiagen), following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

Junqueira

Azeredo-Espin

Paulo

et al. 2016

Sci Rep

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True flies are insects of the order Diptera and encompass one of the most diverse groups of animals on Earth. Within dipterans, Schizophora represents a recent radiation of insects that was used as a model to develop a pipeline for generating complete mitogenomes using various sequencing platforms and strategies. 91 mitogenomes from 32 different species were sequenced and assembled with high fidelity, using amplicon, whole genome shotgun or single molecule sequencing approaches. Based on the novel mitogenomes, we estimate the origin of Schizophora within the Cretaceous-Paleogene (K-Pg) boundary, about 68.3 Ma. Detailed analyses of the blowfly family (Calliphoridae) place its origin at 22 Ma, concomitant with the radiation of grazing mammals. The emergence of ectoparasitism within calliphorids was dated 6.95 Ma for the screwworm fly and 2.3 Ma for the Australian sheep blowfly. Varying population histories were observed for the blowfly Chrysomya megacephala and the housefly Musca domestica samples in our dataset. Whereas blowflies (n = 50) appear to have undergone selective sweeps and/or severe bottlenecks in the New World, houseflies (n = 14) display variation among populations from different zoogeographical zones and low levels of gene flow. The reported high-throughput mitogenomics approach for insects enables new insights into schizophoran diversity and population history of flies.

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Section: Methodsmentioning

confidence: 99%

Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

Junqueira

Azeredo-Espin

Paulo

et al. 2016

Sci Rep

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“…For whole genome sequencing of the control population, high-quality DNA was obtained from seven-dayold pupae, as in this stage, no food remains in their gut. DNA extraction was carried out using a phenol/chlorophorm protocol [26]. Samples were prepared with the Illumina TruSeq DNA Low Sample Protocol with the HiSeq SBS v4 High Output Kit (Illumina, San Diego, USA).…”

Section: Dna and Rna Extraction And Sequencingmentioning

confidence: 99%

Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly

et al. 2020

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Background The emergence of insecticide resistance is a fast-paced example of the evolutionary process of natural selection. In this study, we investigated the molecular basis of resistance in the myiasis-causing fly Cochliomyia hominivorax (Diptera: Calliphoridae) to dimethyl-organophosphate (OP) insecticides. Methods By sequencing the RNA from surviving larvae treated with dimethyl-OP (resistant condition) and non-treated larvae (control condition), we identified genes displaying condition-specific polymorphisms, as well as those differentially expressed. Results Both analyses revealed that resistant individuals have altered expression and allele-specific expression of genes involved in proteolysis (specifically serine-endopeptidase), olfactory perception and cuticle metabolism, among others. We also confirmed that resistant individuals carry almost invariably the Trp251Ser mutation in the esterase E3, known to confer OP and Pyrethroid resistance. Interestingly, genes involved in metabolic and detoxifying processes (notably cytochrome P450s) were found under-expressed in resistant individuals. An exception to this were esterases, which were found up-regulated. Conclusions These observations suggest that reduced penetration and aversion to dimethyl-OP contaminated food may be important complementary strategies of resistant individuals. The specific genes and processes found are an important starting point for future functional studies. Their role in insecticide resistance merits consideration to better the current pest management strategies.

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“…As in most animal species, no length polymorphisms have been reported for C. capitata . However, small size polymorphisms, such as those resulting from variation in copy number of direct tandem duplications in the control region, which seem to be relatively frequent in some insect species such as Drosophila mauritiana (Tsacas and David) (Diptera: Drosophilidae) ( Solignac et al., 1983), D. simulans (Sturtevant) (Diptera: Drosophilidae) ( Baba‐Aissa et al., 1988), Acyrthosiphon pisum (Harris) (Homoptera: Aphidae) ( Barrette et al., 1994) or C. hominivorax (Coquerel) (Diptera: Calliphoridae) ( Vargas and Espin , 1995), may also be present but undetected in C. capitata .…”

Section: 1 Cleavage Mapmentioning

confidence: 99%

Mitochondrial DNA variation in Spanish populations of Ceratitis capitata (Wiedemann) (Tephritidae) and the colonization process

Reyes

Ochando

2004

J Applied Entomology

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Mitochondrial DNA variation in the Mediterranean fruit fly Ceratitis capitata (Wiedemann, Diptera: Tephritidae) was studied in three natural populations from southern, central and eastern Spain by means of restriction fragment length polymorphisms using 22 restriction endonucleases. Nine different haplotypes were found based upon the restriction patterns of the seven polymorphic endonucleases, providing a measure of discrimination between populations (N ST ¼ 0.2462, F ST ¼ 0.154). The observed distribution of haplotypes, corroborated by a parsimonious unrooted tree, suggests an ancient origin for haplotype VII, and a first step in the colonization of the Iberian Peninsula and subsequently the northern and eastern Mediterranean basin, through the Straits of Gibraltar. No relationship can be established between the colonization process for Europe and America.

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Genetic variability in mitochondrial DNA of the screwworm,Cochliomyia hominivorax (Diptera: Calliphoridae), from Brazil

Cited by 14 publications

References 28 publications

Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

Large-scale mitogenomics enables insights into Schizophora (Diptera) radiation and population diversity

Molecular basis of resistance to organophosphate insecticides in the New World screw-worm fly

Mitochondrial DNA variation in Spanish populations of Ceratitis capitata (Wiedemann) (Tephritidae) and the colonization process

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