Genetic Variability in <i>Rhizoctonia solani</i> Isolated from <i>Vitis vinifera</i> Based on Amplified Fragment Length Polymorphism

Abstract

Problem statement: Rhizoctonia solani is a potential grapevine pathogen. In order to develop effective methods of control, it is necessary to document its genetic diversity. Approach: The objective of this study was to analyze the genetic variability of R. solani isolated from the rhizosphere of ungrafted V. vinifera var. perlette seedless planted in Sonora, Mexico using Amplified Fragment Length Polymorphism (AFLP). Results: In the selective amplification using eight primer combinations we obtained a total of 446 AFLP markers with a 100% polymorphism. Out of 41 isolates, 36 different AFLP patterns were observed and five were replicates of the same pattern. The dendrogram shows inter-and intrapopulation similarity indexes of 0.26, 0.98 and 0.31, 0.98, respectively. Six groups emerged from the principal components analysis, five of which were clearly defined, while the other one was spread out. Conclusion: We conclude that R. solani growing in Sonoran vineyards shows a high degree of genetic variability, even under similar environmental conditions.

GENETIC DIVERSITY OF OKRA (<i>ABELMOSCHUS ESCULENTUS</i> L.) GENOTYPES FROM DIFFERENT AGRO-ECOLOGICAL REGIONS REVEALED BY AMPLIFIED FRAGMENT LENGTH POLYMORPHISM ANALYSIS

“…Therefore, AFLP markers combine the advantages of RFLP's and PCR-based markers. This technology has been adopted for fingerprinting and mapping of different plants (Hussein et al, 2005) as well as some pathogens (Meza-Moller et al, 2011). Omahinmin and Osawaru (2005) reported that high degree of wide morphological variation exist among accession of okra which requires further evidence using molecular markers to clarify.…”

AFLP Fingerprinting for Identification of Infra-Species Groups of Rhizoctonia solani and Waitea circinata

“…AFLP analysis on 32 PT and 36 TB isolates placed them into two distinct groups based on their host. A similar genetic diversity study of R. solani AG4 isolates obtained from the rhizosphere of six vineyards in Mexico was reported by Meza-Moller et al [38]. They analysed 41 Rhizoctonia isolates using AFLP markers, which revealed three main groups in the UPGMA dendrogram and six groups from principal component analysis.…”

Unraveling Techniques for Plant Microbiome Structure Analysis

“…The complexity in homologous markers (alleles), representing this method less applicable for accurate research. Conversely the promptness and simplicity with reliability marks which could be created by AFLPs were making this method as a powerful molecular tool kit for ecologists and evolutionary biologists [65,66].…”