Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil

Santini, Annalisa; Magalhães, Juliana Teixeira de; Cascardo, Júlio Cézar M.; Corrêa, Ronan Xavier

doi:10.4238/gmr.15027955

Cited by 2 publications

(2 citation statements)

References 18 publications

(23 reference statements)

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“…Bacteria from the genus Chromobacterium are highly abundant in nature and have been isolated from a wide variety of environments (39)(40)(41)(42)(43)(44). As such, any insecticidal compounds they produce are likely already present in nature, although not at the levels that would be found during a field trial of a Chromobacterium-based biopesticide.…”

Section: Discussionmentioning

confidence: 99%

A Nonlive Preparation of Chromobacterium sp. Panama (Csp_P) Is a Highly Effective Larval Mosquito Biopesticide

Caragata

Otero

Carlson

et al. 2020

Appl Environ Microbiol

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Given the continued high prevalence of mosquito-transmitted diseases, there is a clear need to develop novel disease and vector control strategies. Biopesticides of microbial origin represent a promising source of new approaches to target disease-transmitting mosquito populations. Here, we describe the development and characterization of a novel mosquito biopesticide, derived from an air-dried, nonlive preparation of the bacterium Chromobacterium sp. Panama (family: Neisseriaceae). This preparation rapidly and effectively kills the larvae of prominent mosquito vectors, including the dengue and Zika vector Aedes aegypti and the human malaria vector Anopheles gambiae. During semi-field trials in Puerto Rico, we observed high efficacy of the biopesticide against field-derived A. aegypti populations, and against A. aegypti and Culex species larvae in natural breeding water, indicating the suitability of the biopesticide for use under more natural conditions. In addition to high efficacy, the nonlive Csp_P biopesticide has a low effective dose, a long shelf life, and high heat stability and can be incorporated into attractive larval baits, all of which are desirable characteristics for a biopesticide. IMPORTANCE We have developed a novel preparation to kill mosquitoes from an abundant soil bacterium, Chromobacterium sp. Panama. This preparation is an air-dried powder containing no live bacteria, and it can be incorporated into an attractive bait and fed directly to mosquito larvae. We demonstrate that the preparation has broad spectrum activity against the larval form of the mosquitoes responsible for the transmission of malaria and the dengue, chikungunya, yellow fever, West Nile, and Zika viruses, as well as mosquito larvae that are already resistant to commonly used mosquitocidal chemicals. Our preparation possesses many favorable traits: it kills at a low dosage, and it does not lose activity when exposed to high temperatures, all of which suggest that this preparation could eventually become an effective new tool for controlling mosquitoes and the diseases they spread.

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Section: Discussionmentioning

confidence: 99%

A Nonlive Preparation of Chromobacterium sp. Panama (Csp_P) Is a Highly Effective Larval Mosquito Biopesticide

Caragata

Otero

Carlson

et al. 2020

Appl Environ Microbiol

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“…New therapeutic approaches would be needed to bring meaningful clinical benefit to patients (Romano et al, ; Rudin & Poirier, ; Santini, Magalhaes, Cascardo, & Correa, ). The real possibility to increase patient's survival is to identify new biomarkers or noninvasive device to differentiate benign from malignant lung lesions to obtain an early diagnosis and an accurate staging of NSCLC (Chimenti et al, ; Di Domenico & Giordano, ; Lamberti et al, ).…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle‐biomolecular corona: A new approach for the early detection of non‐small‐cell lung cancer

Domenico

Pozzi

Palchetti

et al. 2018

Journal Cellular Physiology

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Lung cancer (LC) is the most common type of cancer and the second cause of death worldwide in men and women after cardiovascular diseases. Non‐small‐cell lung cancer (NSCLC) is the most frequent type of LC occurring in 85% of cases. Developing new methods for early detection of NSCLC could substantially increase the chances of survival and, therefore, is an urgent task for current research. Nowadays, explosion in nanotechnology offers unprecedented opportunities for therapeutics and diagnosis applications. In this context, exploiting the bio‐nano‐interactions between nanoparticles (NPs) and biological fluids is an emerging field of research. Upon contact with biofluids, NPs are covered by a biomolecular coating referred to as “biomolecular corona” (BC). In this study, we exploited BC for discriminating between NSCLC patients and healthy volunteers. Blood samples from 10 NSCLC patients and 5 subjects without malignancy were allowed to interact with negatively charged lipid NPs, leading to the formation of a BC at the NP surface. After isolation, BCs were characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE). We found that the BCs of NSCLC patients was significantly different from that of healthy individuals. Statistical analysis of SDS‐PAGE results allowed discriminating between NSCLC cancer patients and healthy subjects with 80% specificity, 80% sensitivity and a total discriminate correctness rate of 80%. While the results of the present investigation cannot be conclusive due to the small size of the data set, we have shown that exploitation of the BC is a promising approach for the early diagnosis of NSCLC.

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Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil

Cited by 2 publications

References 18 publications

A Nonlive Preparation of Chromobacterium sp. Panama (Csp_P) Is a Highly Effective Larval Mosquito Biopesticide

A Nonlive Preparation of Chromobacterium sp. Panama (Csp_P) Is a Highly Effective Larval Mosquito Biopesticide

Nanoparticle‐biomolecular corona: A new approach for the early detection of non‐small‐cell lung cancer

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