Genetic variability in Hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (Sprattus sprattus) of different geographical areas of the northeastern Atlantic

Klimpel, Sven; Kleinertz, Sonja; Hanel, Reinhold; Rückert, Sonja

doi:10.1007/s00436-007-0662-0

Cited by 28 publications

(24 citation statements)

References 24 publications

(32 reference statements)

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“…by Paraguassú et al (2002). In species of Hysterothylacium, the excretory pore is located in the region of the nerve ring, thus differing from species of Contracaecum, in which it is located near the ventral interlabia (DEARDORFF; OVERSTREET, 1981;RUKERT., 2005;KLIMPEL et al, 2007;KNOFF et al, 2012;BORGES et al, 2012). According to Smith and Wooten (1978), although it is difficult to identify species based on morphological characters alone, it is even more difficult in relation to the larval stages.…”

Section: Discussionmentioning

confidence: 99%

Parasite community of Pagrus pagrus(Sparidae) from Rio de Janeiro, Brazil: evidence of temporal stability

Soares

Vieira

Luque

2014

Rev. Bras. Parasitol. Vet.

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One hundred specimens of Pagrus pagrus were necropsied for parasitological study between January and February 2012. These data were compared with data from 90 specimens that had been examined in the year 2000. The fish were collected from Cabo Frio (21-23 ° S, 41-45 ° W), in the state of Rio de Janeiro, Brazil. In the current study a total of 19 parasite species were collected. Sixteen of these were collected in the samples of both 2012 and 2000, thus totaling 25 parasite species in the two studies. All the results were similar to those of the previous study with regard to: dominance of anisakid nematode larvae; correlation between abundance of parasites and host body size; absence of correlations between the size of parasite infrapopulations and host sex; and scarcity of interspecific associations. The only difference between the studies was in the abundance values for Anisakidae, which could be correlated with the seasonal factor of the collection period, which was concentrated in the summer. This is also the period of the South Atlantic Central Water (SACW) phenomenon, during which there is greater host reproductive activity. Therefore, significant stability of the metazoan community structure of P. pagrus was shown.Keywords: Pagrus pagrus, parasites, community structure, marine fish, temporal stability. ResumoCem exemplares de Pagrus pagrus foram necropsiados entre janeiro e fevereiro de 2012 para estudos parasitológicos. Esses dados foram comparados com dados de 90 espécimes examinados no ano 2000. Os peixes foram coletados em Cabo Frio (21-23 ° S, 41-45 ° W), no Estado do Rio de Janeiro, Brasil. No presente estudo, foram coletados um total de 19 espécies de parasitos, 16 delas foram coletadas em comum nas amostras de 2012 e 2000, dando um total de 25 espécies de parasitos. Todos os resultados foram semelhantes aos do estudo anterior em: dominância das larvas de anisaquídeos; correlação entre a abundância de parasitos e comprimento total do corpo dos hospedeiros; ausência de correlações entre o tamanho das infrapopulações parasitárias e sexo do hospedeiro e a escassez de associações interespecíficas. A única diferença entre os estudos reside nos valores da abundância de nematóides anisaquídeos, o que poderia ser correlacionado com o fator sazonal do período de coleta que foi concentrada no verão, período que coincide com o fenômeno da Água Central do Atlântico Sul (ACAS), no qual o hospedeiro apresenta maior atividade reprodutiva. Portanto, uma significativa estabilidade da estrutura da comunidade de parasitos de P. pagrus foi evidenciada.Palavras-chave: Pagrus pagrus, parasitas, estrutura da comunidade, peixe marinho, estabilidade temporal.

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Section: Discussionmentioning

confidence: 99%

Parasite community of Pagrus pagrus(Sparidae) from Rio de Janeiro, Brazil: evidence of temporal stability

Soares

Vieira

Luque

2014

Rev. Bras. Parasitol. Vet.

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“…the data only include positive hits. Studies that set out to search for cryptic helminth species but found none do exist [18], but are probably under-represented in the literature. It is therefore difficult to determine the frequency at which one described taxon consists of cryptic species, and all inferences rest solely on cases where cryptic species have been identified.…”

Section: Discussionmentioning

confidence: 99%

Uneven distribution of cryptic diversity among higher taxa of parasitic worms

Poulin

2010

Biol. Lett.

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Cryptic species cause problems for estimates of biodiversity. In the case of parasites, cryptic species also plague efforts to detect potential zoonotic diseases or invasive pathogens. It is crucial to determine whether the likelihood of finding cryptic species differs among higher parasite taxa, to better calibrate estimates of diversity and monitor diseases. Using published reports of cryptic species of helminth parasites identified using molecular tools, I show that the number of species found is strongly related to the number of parasite individuals sequenced, weakly influenced by the number of host species from which parasites were obtained, and unaffected by the genetic markers used. After correction for these factors, more cryptic species of trematodes are found than in other helminth taxa. Although several features distinguish trematodes from other helminths, it is probable that our inability to discriminate among sibling species of trematodes results from their lack of structures serving as species-specific morphological markers. The available data suggest that current estimates of helminth diversity may need to be doubled (tripled for trematodes) to better reflect extant diversity.

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“…The larvae were then identified according to morphological features (Yoshinaga et al 1987, Petter & Maillard 1988. No molecular techniques were needed, since no cryptic sibling species were detected by Klimpel et al (2007). Next, a number of larvae were frozen (-80°C) for enzyme determination (L3-0h).…”

Section: Methodsmentioning

confidence: 99%

Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

Malagón

Adroher

López³

et al. 2010

Dis. Aquat. Org.

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Proteases play a vital role in both the life cycle of parasites and the parasite-host relationship and are considered important virulence factors. In the present study, the presence of proteases with collagenolytic activity was investigated in the fish nematode Hysterothylacium aduncum during in vitro development. Collagenolytic activity was found in all studied developmental stages of the nematode (third [L3] and fourth [L4] larval stages and adults). In L3, the activity was maximum at pH 6.5 and, in the other stages, at 7.0. Pepsin is known to favour in vitro development of the worm, but, in this study, collagenolytic activity was shown to be significantly greater when no pepsin was added to the culture medium (at pH 6.5, p = 0.011). At pH 7.0, most activity was observed in the immature adult, after the final moult, suggesting that the collagenolytic activity may be involved in remodelling of the cuticle and in sexual maturity. On the other hand, at pH 6.5, activity may be related to tissue migration by L3 within the host. Using specific inhibitors, it was demonstrated that most of the collagenolytic activity detected in all the developmental stages was due to metalloproteases (40 to 100%), although serine proteases were also detected in L4 and adults (10 to 30%).

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Genetic variability in Hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (Sprattus sprattus) of different geographical areas of the northeastern Atlantic

Cited by 28 publications

References 24 publications

Parasite community of Pagrus pagrus(Sparidae) from Rio de Janeiro, Brazil: evidence of temporal stability

Parasite community of Pagrus pagrus(Sparidae) from Rio de Janeiro, Brazil: evidence of temporal stability

Uneven distribution of cryptic diversity among higher taxa of parasitic worms

Collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite Hysterothylacium aduncum (Nematoda: Anisakidae)

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