Genetic Variability in Hesperozygis ringens Benth. (Lamiaceae), an Endangered Aromatic and Medicinal Plant of Southern Brazil

Fracaro, Fernando; Echeverrigaray, Sérgio

doi:10.1007/s10528-006-9044-z

Cited by 29 publications

(14 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hesperozygis ringens (Benth.) Epling (Lamiaceae) is a woody herb, 20 to 50 cm high, native to rocky fields of the Pampa biome with a very branched stem (Fracaro and Echeverrigaray, 2006;Von Poser et al, 1996). Some studies have described a high production of essential oil by its leaves, with pulegone as major component (Ribeiro et al, 2010;Silva et al, 2014;Toni et al, 2014;Von Poser et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling.

et al. 2016

View full text Add to dashboard Cite

This study was developed to evaluate the effect of seasonality on the yield and chemical composition of the essential oil (EO) of Hesperozygis ringens (Benth.) Epling, a native species from the Brazilian Pampa. Leaves were collected from four specimens of a single population in each of the four seasons for a year and were extracted in triplicate by hydro-distillation for 2 hours. The yield of EO (% w/w) was calculated on fresh weight basis (FWB), and the 16 oil samples were analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) were used as statistical tools to evaluate differences in chemical composition. The highest yields were obtained in autumn, spring and summer (2.32-4.38%), while the lowest yields were detected in winter, ranging from 1.15 to 1.91%. Oxygenated monoterpenoids were the predominant class of chemical constituents in the EO obtained in all seasons, showing the highest contents in autumn and summer, and pulegone was identified as a major compound, whose contents varied between 54.13 and 81.17%. The EO samples were divided into three chemical groups by HCA and PCA and were assigned to the same group, except for the three samples gathered in winter. The results showed a seasonal influence on the yield and chemical composition of the EO.Keywords: Lamiaceae, "espanta-pulga", pulegone, oxygenated monoterpenoids. Variabilidade sazonal do óleo essencial deHesperozygis ringens (Benth.) Epling. ResumoEste estudo foi desenvolvido a fim de avaliar o efeito da sazonalidade no rendimento e composição química do óleo essencial (OE) de Hesperozygis ringens (Benth.) Epling., uma espécie nativa do Pampa brasileiro. Folhas foram coletadas de quatro indivíduos de uma mesma população, em cada uma das quatro estações de um ano, e foram extraídas em triplicada por hidrodestilação durante 2 horas. O rendimento do OE (% m/m) foi calculado considerando a base fresca (BF) e as 16 amostras de óleo foram analisadas por cromatografia gasosa acoplada à espectrometria de massas (CG-EM) e cromatografia gasosa com detector de ionização de chamas (CG-DIC). Análise Hierárquica de Cluster (AHC) e Análise de Componentes Principais (ACP) foram utilizadas como ferramentas estatísticas para avaliar as diferenças na composição química. Os maiores rendimentos foram obtidos no outono, primavera e verão (2,32-4,38%), enquanto que os menores foram detectados no inverno, variando de 1,15 até 1,91%. Os monoterpenoides oxigenados foram a classe predominante dos constituintes do OE obtido em todas as estações, apresentando os maiores teores no outono e no verão, e a pulegona foi identificada como o constituinte majoritário, cujos teores variaram entre 54,13 e 81,17%. As amostras de OE foram divididas em três grupos químicos por AHC e ACP e foram classificadas no mesmo grupo, com exceção de três amostras coletadas no inverno. Os resultados demonstraram influência sazonal no rendimento e...

show abstract

Section: Introductionmentioning

confidence: 99%

Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling.

et al. 2016

View full text Add to dashboard Cite

show abstract

“…DNA-based molecular markers have the advantage of being free from environmental modulation. Inter simple sequence repeats (ISSR) based on PCR amplification have been widely used for the study of population genetics in several medicinal plants (Bornet et al, 2002;Chen et al, 2006;Fracaro and Echeverrigaray, 2006;Li and Jin, 2008). ISSR primers of repeat motifs (microsatellites) that are abundant and dispersed throughout genomes anchor at either the 5' or 3' end with one or a few specific nucleotides.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Kang¹,

Suresh²,

Lee³

et al. 2013

Korean Journal of Plant Resources

View full text Add to dashboard Cite

-Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity (HT) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles (NE) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei's genetic diversity (H) mean value was 0.218, and Shannon's information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei's genetic diversity, and Shannon's information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.

show abstract

“…Fracaro and Echeverrigaray (2006) observed high genetic diversity in a study with Hesperozygis ringens Benth. plants collected in different regions of the South of Brazil.…”

Section: Discussionmentioning

confidence: 91%

Research Article Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers.

et al. 2017

View full text Add to dashboard Cite

ABSTRACT. Hyptis pectinata, popularly known as 'sambacaitá' or 'canudinho', is a medicinal and aromatic species widely used in the Brazilian Northeast. In Sergipe, the excessive extraction of natural resources may reduce the genetic variability of native plants. Thus, molecular markers have frequently been applied to the characterization of genetic diversity as the basis for germplasm conservation and breeding programs. The objective of the present study was to evaluate the genetic diversity of H. pectinata plants collected in different municipalities of the State of Sergipe using ISSR molecular markers. Thirty-four primers were tested, nine of which were selected for providing reproducible and analyzable amplification products, resulting in 67 polymorphic bands. The expected heterozygosity ranged from 0.32 to 0.45, with a mean of 0.39. Polymorphism information content was of 0.49, which classifies the markers as moderately informative. A dendrogram was constructed

show abstract

Genetic Variability in Hesperozygis ringens Benth. (Lamiaceae), an Endangered Aromatic and Medicinal Plant of Southern Brazil

Cited by 29 publications

References 26 publications

Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling.

Seasonal variability of the essential oil of Hesperozygis ringens (Benth.) Epling.

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Research Article Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers.

Contact Info

Product

Resources

About