Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD)

Voigt, Kerstin; Schleier, S.; Brückner, B.

doi:10.1007/bf00314443

Cited by 73 publications

(40 citation statements)

References 47 publications

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“…G. fujikuroi MP-A was represented by three strains isolated from local rice varieties from Lamjung district. This mating population is commonly associated with Nepalese maize (2, 6), but there have been few reports of its isolation from rice (15,34). A cropping pattern of rice after maize on small farms in the foothills may facilitate movement of this pathogen from maize to rice.…”

Section: Discussionmentioning

confidence: 99%

“…The strains were tested for male fertility once on carrot agar (11) and once on slants of 1% water agar with pieces of autoclaved rice straw. Both of these substrates have been reported to be favorable for crossing MP-C, but carrot agar was usually more effective than rice straw in this study (9,34). Mating tests were incubated in an alternating 12-h, 25°C light/20°C dark schedule.…”

Section: Methodsmentioning

confidence: 96%

“…Mating population C (MP-C) (anamorph, Fusarium fujikuroi) (28) was first identified in 1977 among strains from rice from Taiwan (9). More recently, mating population A (MP-A) (anamorph, Fusarium verticillioides [synonym, F. moniliforme]) and mating population D (MP-D) (anamorph, Fusarium proliferatum) have been isolated from rice from Asia, and MP-D has been isolated from rice from Africa, Australia, and the United States (2,7,34). Thus, more than one species of Fusarium may be able to infect rice and cause symptoms of bakanae disease.…”

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confidence: 99%

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Fusarium Species from Nepalese Rice and Production of Mycotoxins and Gibberellic Acid by Selected Species

Desjardins

Manandhar

Plattner

et al. 2000

Appl Environ Microbiol

209

136

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Infection of cereal grains with Fusarium species can cause contamination with mycotoxins that affect human and animal health. To determine the potential for mycotoxin contamination, we isolated Fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the Nepal Himalaya. The predominant Fusarium species in surface-disinfested seeds with husks were species of the Gibberella fujikuroi complex, including G. fujikuroi mating population A (anamorph, Fusarium verticillioides), G. fujikuroi mating population C (anamorph, Fusarium fujikuroi), and G. fujikuroi mating population D (anamorph, Fusarium proliferatum). The widespread occurrence of mating population D suggests that its role in the complex symptoms of bakanae disease of rice may be significant. Other common species were Gibberella zeae (anamorph, Fusarium graminearum) and Fusarium semitectum, with Fusarium acuminatum, Fusarium anguioides, Fusarium avenaceum, Fusarium chlamydosporum, Fusarium equiseti, and Fusarium oxysporum occasionally present. Strains of mating population C produced beauvericin, moniliformin, and gibberellic acid, but little or no fumonisin, whereas strains of mating population D produced beauvericin, fumonisin, and, usually, moniliformin, but no gibberellic acid. Some strains of G. zeae produced the 8-ketotrichothecene nivalenol, whereas others produced deoxynivalenol. Despite the occurrence of fumonisin-producing strains of mating population D, and of 8-ketotrichothecene-producing strains of G. zeae, Nepalese rice showed no detectable contamination with these mycotoxins. Effective traditional practices for grain drying and storage may prevent contamination of Nepalese rice with Fusarium mycotoxins.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 96%

mentioning

confidence: 99%

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Fusarium Species from Nepalese Rice and Production of Mycotoxins and Gibberellic Acid by Selected Species

Desjardins

Manandhar

Plattner

et al. 2000

Appl Environ Microbiol

209

136

View full text Add to dashboard Cite

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“…It is present in all prokaryote and eukaryote that can be targeted for amplification to identify the pathogen [7]. In contrast, molecular approaches have been developed for fungal systematic studies, including RAPD analysis [14], specific diagnostic PCR primers [15]. Since these molecular studies require a number of laborious steps, including preparation of a DNA template by extraction of DNA from fungi [10].…”

Section: Introductionmentioning

confidence: 99%

Direct Colony Nested-PCR for the Detection of Fusarium oxysporum f. sp. Psidii Causing Wilt Disease in Psidium guajava L.

Mishra¹,

Verma²

2014

Horticulture

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Guava (Psidium guajava L.) is one of the important fruit crops which are grown in tropical and subtropical countries including India. Wilt is an important disease in guava which is caused by Fusarium oxysporum f. sp. psidii (Fop) as a major obstacle for guava fruit production. It is manifested symptomatically with alterations in the development process such as premature shedding of leaves, pre-maturation of fruits, entire/whole tree defoliation and eventually death of the plant. In this study a colony PCR assay was developed for direct amplification of Internal Transcribed Spacer (ITS) region of fungal rDNA of Fop isolates. For this, two sets of primers were developed for amplification of ITS region. . The sensitivity of both the primer pair was ranged up to 10 -6 and 10 -7 dilutions of the pure mycelium suspension. This study provides new insight for rapid, sensitive and specific molecular detection of Fop isolates, and also useful for earlier diagnostic for better management of guava wilt disease.

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“…Entre as técnicas moleculares utilizadas para discriminação de Fusarium spp. destacam-se: Polimorfismo de DNA amplificado ao acaso (RAPD) (Duteau & Leslie, 1991;Ouellet & Seifert, 1993;Voigt et al, 1995;Viljoen et al, 1997), Polimorfismo no comprimento de fragmentos de restrição (RFLP) (Correll et al, 1992;Nicholson et al, 1993), espaços intergênicos transcritos (ITS 1 e ITS 2) (O'Donnell & Cigelnik, 1997;Waalwijk et al, 1996) e dados de seqüências gênicas conservadas (Steenkamp et al, 2000a), Polimorfismo no comprimento de fragmentos amplificados (AFLP) (Jurgenson et al, 2002) e Reação de Polimerase em Cadeia (PCR) (Hue et al, 1999;Steenkamp et al, 2000b). Steenpkamp et al (1999) combinaram as técnicas de PCR-RFLP para a diferenciação dos fungos de F. subglutinans associados a vários hospedeiros (cana-de-açúcar, pinheiro, mangueira e milho).…”

Section: Introductionunclassified

Utilização de marcador molecular SCAR na identificação de Fusarium subglutinans, agente causal da malformação da mangueira

et al. 2007

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RESUMO-O gênero Fusarium é responsável por doenças em diversas plantas economicamente importantes. Entre estas doenças, destaca-se a malformação da mangueira, causada pelo fungo Fusarium subglutinans. O objetivo deste trabalho foi desenvolver oligonucleotídeos iniciadores para reação em cadeia da polimerase (PCR), específicos para o fungo F. subglutinans da mangueira. A amplificação de DNA de oito Fusarium spp. de diferentes hospedeiros, usando o oligonucleotídeo randômico UBC-41 (TTAACCGGGG), produziu um fragmento de aproximadamente 1.300 pb somente para o fungo da mangueira. Tendo em vista que padrões de bandeamento por RAPD não são considerados confiáveis devido à baixa reprodutibilidade dos resultados, o fragmento diferencial foi eluído do gel de agarose, purificado, clonado e seqüenciado. As seqüências nucleotídicas foram utilizadas para identificar e sintetizar quatro pares de oligonucleotídeos específicos, denominados Fs 5, Fs 13, Fs 14 e Fs 15. DNAs de Fusarium spp. de outros hospedeiros (alho, amendoim, cana-de-açúcar, ciclâmen, ervilha, melão e trigo), da planta de mangueira cv. Tommy Atkins sadia e de outros cinco isolados de F. subglutinans de mangueira sintomática, foram submetidos à amplificação com os pares de oligonucleotídeos. Fragmentos amplificados foram visualizados somente para F. subglutinans de mangueira, demonstrando assim a especificidade dos oligonucleotídeos SCAR desenhados. Termos de Indexação: oligonucleotídeos iniciadores, Mangifera indica, marcadores moleculares, RAPD-PCR. USE OF MOLECULAR MARKER SCAR IN THE IDENTIFICATION OF Fusarium subglutinans,CAUSAL AGENT OF MANGO MALFORMATIONABSTRACT-The Fusarium genus is responsible for serious diseases in many economically important crops. Among these diseases it is distinguished the mango flower malformation, caused by the fungus Fusarium subglutinans. The objective of this work was to develop specific oligonucleotide primers for the polymerase chain reaction (PCR) targeting the mango flower malformation by fungus F. subglutinans. The DNA amplification of eight Fusarium spp. collected from different hosts making the use of a random oligonucleotide primer UBC-41 generated a fragment of approximately 1300 pb in size, specifically for the mango flower malformation fungus (Fusarium subglutinans). Since standard RAPD banding patterns are not considered reliable because of their low results of reproducibility, the distinctive fragment was eluted off the agarose gel, purified, cloned and then sequenced. The nucleotide sequences were used to identify and also to synthesize four pairs of specific oligonucleotide named herein Fs 5, Fs 13, Fs 14 and Fs 15. Other Fusarium spp. DNAs sampled from other hosts (garlic, peanut, sugarcane, cyclamen, pea, melon and wheat), from a healthy mango tree cv. Tommy Atkins and other six isolates from symptomatic mango plants were submitted to PCR amplification with these pairs of oligonucleotide. Only fragments from the mango tree fungus Fusarium subglutinans were visualized, showing this way the SCAR oligonucleotid...

show abstract

Genetic variability in Gibberella fujikuroi and some related species of the genus Fusarium based on random amplification of polymorphic DNA (RAPD)

Cited by 73 publications

References 47 publications

Fusarium Species from Nepalese Rice and Production of Mycotoxins and Gibberellic Acid by Selected Species

Fusarium Species from Nepalese Rice and Production of Mycotoxins and Gibberellic Acid by Selected Species

Direct Colony Nested-PCR for the Detection of Fusarium oxysporum f. sp. Psidii Causing Wilt Disease in Psidium guajava L.

Utilização de marcador molecular SCAR na identificação de Fusarium subglutinans, agente causal da malformação da mangueira

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