Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation

Rojas, Lázara; Fraga, Jorge; Sariego, Idalia

doi:10.1016/j.meegid.2003.12.003

Cited by 30 publications

(16 citation statements)

References 15 publications

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“…Currently, there is no "gold standard" method for genotyping isolates of T. vaginalis. A number of methods have been used in previous attempts to characterize the genetic diversity and population structure of T. vaginalis including isoenzyme analysis, repetitive sequence hybridization, random amplified polymorphic DNA, restriction fragment length polymorphism (RFLP), sequence polymorphisms in rRNA genes and intergenic regions, pulsed-field gel electrophoresis, and antigenic characterization (3,17,26,43,49,50,58,60,64,65). These methods have variable levels of reproducibility and precision in determining the genetic relatedness of isolates.…”

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confidence: 99%

Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing

et al. 2012

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dIn this study, we introduce a multilocus sequence typing (MLST) scheme, comprised of seven single-copy housekeeping genes, to genetically characterize Trichomonas vaginalis. Sixty-eight historical and recent isolates of T. vaginalis were sampled from the American Type Culture Collection and female patients at area health care facilities, respectively, to assess the usefulness of this typing method. Forty-three polymorphic nucleotide sites, 51 different alleles, and 60 sequence types were distinguished among the 68 isolates, revealing a diverse T. vaginalis population. Moreover, this discriminatory MLST scheme retains the ability to identify epidemiologically linked isolates such as those collected from sexual partners. Population genetic and phylogenetic analyses determined that T. vaginalis population structure is strongly influenced by recombination and is composed of two separate populations that may be nonclonal. MLST is useful for investigating the epidemiology, genetic diversity, and population structure of T. vaginalis.

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confidence: 99%

Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing

et al. 2012

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“…The isolate T-H7 of T. vaginalis was axenized from a symptomatic woman following the methodology described by Rojas et al (2004). Parasites were cultured in TYI (Diamond et al 1978) supplemented with heat-inactivated bovine serum at a final concentration of 10%, under anaerobic conditions, at 37 C. Cultures at exponential phase were used for the experiments.…”

Section: Parasites Culturesmentioning

confidence: 99%

Antiprotozoal screening of the Cuban native plant Scutellaria havanensis

Valdés

Fidalgo

Ramos

et al. 2016

Pharmaceutical Biology

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Context: Scutellaria havanensis Jacq. (Lamiaceae) is a native medicinal herb with a history of use in Cuba.Objective: This study screens the antiprotozoal activity of S. havanensis. Materials and methods: Chloroform and methanol extracts from leaves and stems were evaluated in vitro at doses between 0.015 and 200 lg/mL against protozoan parasites: Plasmodium berghei, Trichomonas vaginalis and Leishmania amazonensis. Chloroform and methanol extracts were characterized by GC/MS. Cytotoxicity against mouse peritoneal macrophages was tested in parallel. Results: Scutellaria havanensis extracts exhibited IC 50 values between 7.7 and 32.2 lg/mL against trophozoites of P. berghei and T. vaginalis; while the extracts were inactive against L. amazonensis promastigotes. Trichomonicidal activity of methanol extract exhibited the best selectivity but chloroform extract showed the highest antiplasmodial, trichomonicidal and cytotoxic activity. The majority of compounds in the chloroform extract were hydroxy and/or methoxyflavones (77.96%), in particular, wogonin (48.27%). In methanol extract, wogonin (5.89%) was detected. Trichomonicidal effect of wogonin was moderate (IC 50 ¼ 56 lM) and unspecific with respect to macrophages (SI ¼ 2). On the contrary, antiplasmodial activity of wogonin were particularly active (IC 50 ¼ 15 lM) demonstrating a higher selectivity index (SI ¼ 7.4). Conclusions: Wogonin is an active principle compound of the chloroform extract of S. havanensis against P. berghei and T. vaginalis trophozoites, whereas the methanol extract of S. havanensis should be investigated more deeply as a trichomonicide. Our findings suggest that wogonin is potentially useful for the development of antimalarial alternative treatments. ARTICLE HISTORY

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“…Initial investigations into T. vaginalis genetic diversity used RAPD analysis (Rapid Amplification of Polymorphic DNA) 7 . Drawbacks of this method are a lack of reproducibility between laboratories and a requirement for DNA from axenic organisms, since DNA from any source can act as a template for amplification when using random primer annealing.…”

Section: Introductionmentioning

confidence: 99%

A preliminary investigation of microsatellite-based genotyping in Trichomonas vaginalis

Prokopi

Chatzitheodorou

Ackers

et al. 2011

Transactions of the Royal Society of Tropical Medicine and Hygi

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Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation

Cited by 30 publications

References 15 publications

Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing

Genetic Characterization of Trichomonas vaginalis Isolates by Use of Multilocus Sequence Typing

Antiprotozoal screening of the Cuban native plant Scutellaria havanensis

A preliminary investigation of microsatellite-based genotyping in Trichomonas vaginalis

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