Genetic Variability Assessed by Microsatellites in a Breeding Program of Pacific White Shrimp (Litopenaeus vannamei)

Cruz, Pedro; Ibarra, Ana M.; Mejı́a-Ruı́z, Humberto; Gaffney, Patrick M.; Pérez‐Enríquez, Ricardo

doi:10.1007/s10126-003-0017-5

Cited by 46 publications

(34 citation statements)

References 18 publications

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“…In the three generations studied most loci showed a significant deviation from the Hardy-Weinberg equilibrium (p < 0.012) when the Bonferroni correction (Rice, 1989) was applied and the F IS values obtained (Table 1) could be probably due to genetic drift, inbreeding and selection effects. Heterozygote deficit was observed for all studied generations and could be related to null alleles, preferential mating and sampling errors (Cruz et al, 2003). The mean observed heterozygosity (H o ) values varied from 0.37 to 0.60 and were significantly different (p = 0.018) between the three generations but similar to the mean values reported for diverse cultured shrimp species (reviewed by Benzie, 2000).…”

Section: Resultssupporting

confidence: 75%

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F 5 , F 6 and F 7 ) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic variability over the three generations studied. One possible reason for this is that the breeding conditions of this lineage were good enough to prevent any significant loss of genetic variability. However, three generations may have been insufficient to produce detectable changes in genetic frequencies in the loci studied. Alternatively, the microsatellite loci may have been non-neutral (biased) and related to the conditions in which the shrimps were kept, resulting in a similar allele pool in respect to these four microsatellites over the three generations studied. Any generalizations regarding microsatellite variation in closed shrimp lines may thus be incomplete and should be carefully analyzed.

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Section: Resultssupporting

confidence: 75%

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

2007

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“…The specific loci were selected from the literature as the most highly diverse for this species (Cruz et al 2002(Cruz et al , 2004Valles-Jiménez et al 2005;Machado-Tamayo 2006;Garcia and Alcivar-Waren 2007;Luvesuto et al 2007). We had 6 and 4 % reduction of H o in Amiri-Gomishan (0.479-0.450) and Amiri-Gorgeaj (0.479-0.458) populations that represent a 5.4 and 3.6 % increase in inbreeding, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Four previously described microsatellite markers TUDGLv5-7.33, TUDGLv7-9.17, TUDGLv1-3.224 (Garcia and Alcivar-Waren 2007), and P van 1758 (Cruz et al 2004) have been chosen. Polymerase chain reactions were conducted in Gene Amp PCR system 9600 thermocycler under the following conditions: 94°C for 5 min, followed by 35 cycles of 94°C for 1 min, 44-60°C for 1 min, and 72°C for 1 min, followed by a final extension step of 72°C for 10 min.…”

Section: Dna Extraction and Microsatellite Amplificationmentioning

confidence: 99%

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

2015

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Genetic diversity is vital for the maintenance of genetic pool in cultured shrimps. In order to estimate the current status of genetic diversity in Litopenaeus vannamei shrimp in Iran, as an exotic species, a total of 45 individuals from Amiri and Gorgeaj farms in Jask port of Hormozgan province and one hatchery in Gomishan city of Golestan province, were detected using four microsatellite loci. The number of alleles per locus was 5-10, and the mean effective number of alleles (N e ) across populations and loci ranged from 4.834 to 5.148. The overall mean observed heterozygosity (H o ) ranged from 0.450 to 0.479, which was lower than the expected one (0.789-0.794). There was nothing remarkable about any of the allele frequencies across populations or loci. The mean inbreeding coefficient (F IS ) and pairwise genetic differentiation (F ST ) among populations were 41.6 % and 0.133, respectively. The three studied populations departed from Hardy-Weinberg equilibrium (HWE). Analysis of molecular variance revealed 14 % variability among and 86 % within populations. However, considering departing from HWE and the high F IS and F, the moderate pairwise F ST values, importance of introducing genetically diverse broodstock and monitoring to control inbreeding is discussed.

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“…Over the past decade, microsatellites have produced promising results in studies of genetic variation in many marine species (Castro et al 2003;Cruz et al 2004;Chistiakov et al 2006;An et al 2010). Recently, numerous microsatellite markers have been identified for endangered species that can serve as *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

Development and characterization of microsatellite markers for an endangered species,Epinephelus bruneus, to establish a conservation program

Kim

Lee

et al. 2012

Animal Cells and Systems

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Kelp grouper (Epinephelus bruneus Bloch 1793) is a commercially important fish in Korea. In recent years, the catch of kelp grouper in the coastal waters of Korea has significantly declined. Despite its importance, little is known about its genetic diversity and conservation efforts are hampered. In this study, we isolated and characterized 12 microsatellite loci using an enrichment method based on magnetic/biotin capture of microsatellite sequences from a size-selected genomic library. All loci were readily amplified and contained TG/CA denucleotide repeats. To characterize each locus, 30 individuals from a natural E. bruneus population in the coastal waters of Jeju Island, Korea, were genotyped. All loci except three, KEm118, KEm154, and KEm219, were polymorphic, with an average of 8.1 alleles per locus (range 2Á18). The mean observed and expected heterozygosities were 0.47 (range 0.19Á1.00) and 0.61 (range 0.29Á0.92), respectively. A significant deviation from Hardy-Weinberg equilibrium was observed at three loci (KEm134, KEm184, and KEm283). These findings will be useful for effective monitoring and management of genetic variation of kelp grouper as well as for the implementation of a fisheries conservation program.

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Genetic Variability Assessed by Microsatellites in a Breeding Program of Pacific White Shrimp (Litopenaeus vannamei)

Cited by 46 publications

References 18 publications

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae)

Genetic diversity status of Pacific white shrimp (Litopenaeus vannamei) using SSR markers in Iran

Development and characterization of microsatellite markers for an endangered species,Epinephelus bruneus, to establish a conservation program

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