Genetic variability and virulence among Verticillium albo-atrum isolates from hop

Radišek, Sebastjan; Jakše, Jernej; Javornik, Branka

doi:10.1007/s10658-006-9061-0

Cited by 45 publications

(35 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The outbreak of lethal wilt caused by Verticillium albo-atrum pathotypes, which was first registered in England and later in Slovenia, but not in other hop growing regions, might be explained by the similar germplasm D in hop cultivars. V. alboatrum pathotypes are known to respond to the selection pressure of hosts with enhanced degrees of resistance and, in the case of the above hop wilt, pathogens have been subjected to similar selection pressures from the hop cultivars, which has resulted in the same virulence phenotype of lethal isolates from the two hop growing regions (Radisek et al 2006).…”

Section: Hop Genetic Structurementioning

confidence: 99%

Genetic structure and differentiation in hop (Humulus lupulus L.) as inferred from microsatellites

et al. 2007

Self Cite

View full text Add to dashboard Cite

A set of 67 wild and cultivated hop accessions, representative of hop diversity, was genotyped with 29 SSR markers in order to investigate the population structure and genetic diversity among hop genotypes. A total of 314 alleles was detected, with an average of 10.8 alleles per locus and an average PIC content of 0.607. Model-based clustering placed the accessions into five germplasm groups. A distance-based tree showed good agreement with five germplasm groups, and additionally assigned accessions omitted from model-based analysis into two additional germplasm groups. The 67 hop accessions were thus subdivided in seven germplasm groups, with three corresponding to major breeding groups and four to wild hops. This finding is in accordance with two biogeographically separated hop germplasms (European and North American origin) and with the known history of the accessions. North American hop germplasm was partitioned into native and cultivated germplasm groups. European germplasm was divided into two groups of hop cultivars representing distinguishable European germplasms and three new groups of native hops, which were differentiated for the first time by this analysis. Admixture analysis showed shares of various ancestries in hop cultivars, mostly congruent with pedigree data, and the introgression of various ancestries in some native hops. The above results have so far given the most detailed insight to date into the population structure of hop diversity, which is important for its effective use in hop breeding.

show abstract

Section: Hop Genetic Structurementioning

confidence: 99%

Genetic structure and differentiation in hop (Humulus lupulus L.) as inferred from microsatellites

et al. 2007

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the ten species currently recognized in Verticillium sensu stricto, [4], [5], V. dahliae is most widespread and most economically important [1], [6], [7], but V. albo-atrum [8], V. alfalfae [9], [10], V. longisporum [11], [12], V. nonalfalfae [13], [14], V. tricorpus [8], [15] and V. zaregamsianum [16] also cause significant losses, V. nubilum causes disease in pathogenicity tests [15], and both V. isaacii and V. klebahnii have been recovered from lettuce and artichoke, respectively [4], [17]. One of the characteristic features of Verticillium species is the formation of resting structures [4].…”

Section: Introductionmentioning

confidence: 99%

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

et al. 2013

View full text Add to dashboard Cite

Accurate species identification is essential for effective plant disease management, but is challenging in fungi including Verticillium sensu stricto (Ascomycota, Sordariomycetes, Plectosphaerellaceae), a small genus of ten species that includes important plant pathogens. Here we present fifteen PCR assays for the identification of all recognized Verticillium species and the three lineages of the diploid hybrid V. longisporum. The assays were based on DNA sequence data from the ribosomal internal transcribed spacer region, and coding and non-coding regions of actin, elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase and tryptophan synthase genes. The eleven single target (simplex) PCR assays resulted in amplicons of diagnostic size for V. alfalfae, V. albo-atrum, V. dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii, V. nonalfalfae, V. nubilum, V. tricorpus, V. zaregamsianum, and Species A1 and Species D1, the two undescribed ancestors of V. longisporum. The four multiple target (multiplex) PCR assays simultaneously differentiated the species or lineages within the following four groups: Verticillium albo-atrum, V. alfalfae and V. nonalfalfae; Verticillium dahliae and V. longisporum lineages A1/D1, A1/D2 and A1/D3; Verticillium dahliae including V. longisporum lineage A1/D3, V. isaacii, V. klebahnii and V. tricorpus; Verticillium isaacii, V. klebahnii and V. tricorpus. Since V. dahliae is a parent of two of the three lineages of the diploid hybrid V. longisporum, no simplex PCR assay is able to differentiate V. dahliae from all V. longisporum lineages. PCR assays were tested with fungal DNA extracts from pure cultures, and were not evaluated for detection and quantification of Verticillium species from plant or soil samples. The DNA sequence alignments are provided and can be used for the design of additional primers.

show abstract

“…V. nonalfalfae , a soil born fungal pathogen, causes serious economic damage in European hop growing regions. Significant efforts have been invested to study the molecular mechanisms of Verticillium wilt in hop and fungus pathogenicity (Radišek et al 2006; Jakše et al 2013; Cregeen et al 2015; Mandelc and Javornik 2015; Flajšman et al 2016; Jakše et al 2018; Marton et al 2018).…”

Section: Discussionmentioning

confidence: 99%

The invisibility cloak: Chitin binding protein ofVerticillium nonalfalfaedisguises fungus from plant chitinases

Volk

Marton

Flajšman

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

During fungal infections, plant cells secrete chitinases that digest chitin in the fungal cell walls. The recognition of released chitin oligomers via lysin motif (LysM)-containing immune receptors results in the activation of defence signalling pathways. We report here that Verticillium nonalfalfae, a hemibiotrophic xylem-invading fungus, prevents this recognition process by secreting a CBM18 (carbohydrate binding motif 18)-chitin binding protein, VnaChtBP, which is transcriptionally activated specifically during the parasitic life stages. VnaChtBP is encoded by the Vna8.213 gene which is highly conserved within the species, suggesting high evolutionary stability and importance for the fungal lifestyle. In a pathogenicity assay, however, Vna8.213 knockout mutants exhibit wilting symptoms similar to the wild type fungus, suggesting that Vna8.213 activity is functionally redundant during fungal infection of hop. In binding assay, recombinant VnaChtBP binds chitin and chitin oligomers in vitro with submicromolar affinity and protects fungal hyphae from degradation by plant chitinases. Using a yeast-two-hybrid assay, homology modelling and molecular docking, we demonstrated that VnaChtBP forms dimers in the absence of ligands and that this interaction is stabilized by the binding of chitin hexamers with a similar preference in the two binding sites. Our data suggest that, in addition to chitin binding LysM (CBM50) and Avr4 (CBM14) fungal effectors, structurally unrelated CBM18 effectors have convergently evolved to prevent hydrolysis of the fungal cell wall against plant chitinases.

show abstract

Genetic variability and virulence among Verticillium albo-atrum isolates from hop

Cited by 45 publications

References 35 publications

Genetic structure and differentiation in hop (Humulus lupulus L.) as inferred from microsatellites

Genetic structure and differentiation in hop (Humulus lupulus L.) as inferred from microsatellites

Identification and Differentiation of Verticillium Species and V. longisporum Lineages by Simplex and Multiplex PCR Assays

The invisibility cloak: Chitin binding protein ofVerticillium nonalfalfaedisguises fungus from plant chitinases

Contact Info

Product

Resources

About