Genetic variability and population structure of dorada (Brycon moorei sinuensis Dahl) in the Sinú River, Córdoba, Colombia

Abstract

Six microsatellite loci, isolated for Piaractus mesopotamicus (Characidae, Serrasalminae) were used to evaluate the genetic variability of the dorada (Brycon moorei sinuensis, Characidae, Bryconinae) population in the Sinú River, Córdoba, Colombia, and to facilitate management recommendations for this species. The six loci were polymorphic, with an average of 9.25 alleles for dimeric loci (Pme2, Pme4, Pme5 and Pme20), and five alleles for trimeric loci. Tests for linkage disequilibrium were used to determine that the six loci had an independent distribution. The observed heterozygosities were lower than the expected ones for all loci, and the population was not in Hardy-Weinberg equilibrium because of heterozygote deficiency. F-statistics indicated inbreeding and a low population substructure. The homogeneity of the population was confirmed by high values of genetic flux and an agglomerated representation of the individuals, based on factor correspondence analysis. Recent bottlenecks were detected for three sample sites under the assumption of an infinite allele mutation model. Proposals for the management of the dorada population are made and validated with a genetic simulation.

Population genetics of the fish Brycon henni (Characiformes: Bryconidae) using species-specific polymorphic microsatellite loci

“…The levels of observed (0.461-0.645) and expected (0.604-0.662) heterozygosities were in the range of average heterozygosity per species across microsatellite loci (H O : 0.189-0.880; H E : 0.486-0.892) found in seven Brycon species (Ashikaga et al, 2015;López, 2006;Matsumoto & Hilsdorf, 2009;Melon-Barroso et al, 2005; and Neotropical Characiforms (Hilsdorf & Hallerman, 2017). Specifically, the average expected B. henni heterozygosities were higher than those observed in B. orbignyanus (Ashikaga et al, 2015), similar to B. moorei sinuensis (López, 2006) and B. hilarii , but lower than B. opalinus (Melon-Barroso et al, 2005), B. insignis (Matsumoto & Hilsdorf, 2009), B. orthotaenia , and B. amazonicus (Oliveira, Santos, Bernardino, Hrbek, & Farias, 2018).…”

“…hilarii , but lower than B. opalinus (Melon-Barroso et al, 2005), B. insignis (Matsumoto & Hilsdorf, 2009), B. orthotaenia , and B. amazonicus (Oliveira, Santos, Bernardino, Hrbek, & Farias, 2018). However, the average observed B. henni heterozygosities in IT and ES were higher than those found in B. hilarii ), B. opalinus (Melon-Barroso et al, 2005, B. moorei sinuensis (López, 2006), and B. orbignyanus (Ashikaga et al, 2015), and lower than B. orthotaenia and B. insignis (Matsumoto & Hilsdorf, 2009). In LM, the average observed B. henni heterozygosities were only higher than those found in B. moorei sinuensis (López, 2006) and B. orbignyanus (Ashikaga et al, 2015).…”

“…Moreover, this species has a short-distance migration range between main river channels and adjacent highgradient streams (lateral migration) (Builes & Urán, 1974;Lasso et al, 2010), a fact that suggests its natural populations are not panmictic along its distribution range. Indeed, genetically structured populations have been found for B. henni (Hurtado-Alarcón, Mancera-Rodríguez, & Saldamando-Benjumea, 2011;Pineda-Santis et al, 2007) and other congeners such as Brycon hilarii (Sanches & Galetti Jr., 2007, Brycon insignis (Matsumoto & Hilsdorf, 2009), Brycon lundii (Wasko & Galetti Jr., 2002), Brycon moorei sinuensis (López, 2006), Brycon opalinus (Melon-Barroso, Hilsdorf, Moreira, Cabello, & Traub-Cseko, 2005), Brycon orbignyanus (Ashikaga, Orsi, Oliveira, Senhorini, & Foresti, 2015;Panarari-Antunes et al, 2011), and Brycon orthotaenia .…”

Utilización De La Biología Molecular Como Medio Para Optimizar La Producción Piscícola Y Repoblamiento De Medios Naturales

“…Con Oncorhynchus masou se analizarón los efectos de ayuno y de re-alimentación tanto en los niveles de ARNm y de la proteína junto con IGF-I y ARN/ADN (KAWAGUCHI et al, 2013). Para Arapaima gigas (HRBEK et al, 2005;HRBEK et al,2007), Brycon amazonicus (PARADA et al,2003, Brycon henni (PINEDA-SANTIS et al,2004;PINEDA-SANTIS et al,2007;MANCERA-RODRÍGUEZ et al,2012),Brycon moorei sinuensis (LÓPEZ, 2003;LÓPEZ et al,2004),Plagioscion squamosissimus (GALLETI et al, 2009), Prochilodus nigricans (MACHADO et al, 2009), Pseudoplatystoma (PERDOMO et al, 2009) se utilizaron estudios de DNA mitocondrial, igualmente para los análisis genéticos y filogenéticos de Prochilodus lineatus, P. nigricans, P. rubrotaeniatus, P. mariae y P. magdalenae (SIVASUNDAR et al, 2001;TURNER et al,2004;ORTÍ et al, 2005), Pseudoplatystoma magdaleniatum (TORRICO et al, 2009);y OCHOA-ORREGO et al (2009) trabajaron 14 especies de peces migratorios del río Magdalena usando el código de barras genético. En Piaractus brachypomus MONTAÑO-ARIAS & FORERO (2003) utilizaron isoenzimas en sus estudios.…”

“…Se realizaron trabajos de variabilidad genética en Brycon moorei sinuensis (LÓPEZ, 2003;LÓPEZ et al,2004), Caquetaia krausii (LAMPREA 2003LAMPREA et al,2004), Prochidolus magdalenae (SANTACRUZ, 2003;SANTACRUZ et al, 2004SANTACRUZ et al, ), bol.cient.mus.hist.nat. 19 (1) enero -junio 2015 Pseudoplatystoma fasciatum, P.magdaleniatum, P. orinocoense, P. punctifer,P.…”