Genetic variability and mycohost association of Ampelomyces quisqualis isolates inferred from phylogenetic analyses of ITS rDNA and actin gene sequences

Park, Ju Hee; Choi, Young Joon; Hong, Seung Beom; Shin, Hyeon Dong

doi:10.1016/j.funbio.2010.01.003

Cited by 35 publications

(56 citation statements)

References 29 publications

(30 reference statements)

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“…Strains were selected according to the fungal host of origin. The selected strains represent the four major clades reported in a recent study by Park et al (2010).…”

Section: Methodsmentioning

confidence: 99%

“…Many molecular analyses based on the internal transcribed spacer (ITS) region of the nuclear ribosomal RNA gene (nrDNA) have revealed considerable genetic diversity among A. quisqualis strains (Angeli et al 2009a;Liang et al 2007;Park et al 2010;Szentiványi et al 2005). Recently, ITS sequences and microsatellite markers have been used to show that a set of A. quisqualis populations found in apple powdery mildew (Podosphaera leucotricha) are quite genetically distinct from populations collected from several other powdery mildew species infecting other plant species (Kiss et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, a thorough investigation of A. quisqualis sensu strictu is lacking. More physiological and phenotypic information is required to complete the picture of the genetic differences that have been identified in previous studies (Kiss et al 2011;Liang et al 2007;Park et al 2010).…”

Section: Introductionmentioning

confidence: 99%

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Existence of different physiological forms within genetically diverse strains of Ampelomyces quisqualis

et al. 2011

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Powdery mildew fungi are parasitized by strains of the genetically distinct Ampelomyces quisqualis. To investigate whether differences in the phylogeny and other cultural, morphological and physiological characteristics of these different strains are related to differences in their geographic origins or the host species from which they were isolated, several A. quisqualis strains isolated from different species of Erysiphaceae collected in different countries and possessing different ITS rDNA sequences were selected and characterized. The results revealed some significant variation among the selected strains, which provides evidence for the existence of different physiological forms within the A. quisqualis species. Two groups that display differential growth on artificial media were identified. These groups also differ in the morphology of their mycelium, but not in the morphology of their pycnidia and conidia. Temperature greatly affected the in vitro growth of the A. quisqualis strains and growth rate was closely correlated to colony color. Differences in the conidial germination of distinct strains were observed during the recognition phase of the parasitic relationship. The germination of each of the investigated strains was greatly stimulated by all of the examined powdery mildew species and not only by the conidia of their original hosts. An Italian strain isolated from grapevine in the Trentino Alto-Adige region was identified as the strain that germinates the most quickly in the presence of powdery mildew conidia. Phylogenetic analysis revealed that these A. quisqualis strains can be classified into five different genetic groups, which generally correlate with the fungal host of origin and morphological and growth characteristics.

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“…Strains were selected according to the fungal host of origin. The selected strains represent the four major clades reported in a recent study by Park et al (2010).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Existence of different physiological forms within genetically diverse strains of Ampelomyces quisqualis

et al. 2011

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“…65 fungal species of eight genera of the order Erysiphales in different regions globally [2]. In Korea, Ampelomyces has been revealed on 19 species of the genus Erysiphe and occurs on a wide range of plants [7]. To our knowledge, there were no previous published literature records of this species as a mycoparasite of powdery mildew disease on sycamore (Platanus occidentalis L.) and crape myrtle (Lagerstroemia indica L.) hosts.…”

Section: Abstract : Ampelomyces Quisqualis Hyperparasite Lagerstroementioning

confidence: 99%

First Report of Ampelomyces quisqualis from Sycamore and Crape Myrtle and Its Potential as a Mycoparasite of Powdery Mildew

Nguyen¹,

Lee²

2016

The Korean Journal of Mycology

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: During screening fungi as potential biological control agents for plant parasitic fungi, a fungal strain, EML-FAM3, was isolated from powdery mildew leaf lesions caused by Erysiphe platani on sycamore (Platanus occidentalis L.), and another strain, EML-FAMC1, from Erysiphe australiana on crape myrtle (Lagerstroemia indica L.). Based on the morphological characteristics and phylogenetic analysis of the internal transcribed spacers (ITS1 and ITS2) and 5.8S rDNA, the strains were identified as Ampelomyces quisqualis. To our knowledge, this is the first report of new mycohosts, E. platani and E. australiana, of the mycoparasite A. quisqualis on sycamore and crape myrtle plants. The hyperparasite may represent the potential for controlling E. platani and E. australiana epidemics. KEYWORDS : Ampelomyces quisqualis, Hyperparasite, Lagerstroemia indica, Platanus occidentalis, Powdery mildewAmpelomyces spp. are common intracellular mycoparasites of powdery mildews. Pycnidial fungi belonging to the haploid ascomycete genus Ampelomyces are common mycoparasites occurring intracellularly in mycelia of powdery mildews (Erysiphaceae) worldwide [1,2]. The natural co-occurrence of A. quisqualis on various Erysiphaceae species has been reported in different geographic regions [3][4][5][6]. Ampelomyces species have been associated with ca. 65 fungal species of eight genera of the order Erysiphales in different regions globally [2]. In Korea, Ampelomyces has been revealed on 19 species of the genus Erysiphe and occurs on a wide range of plants [7]. To our knowledge, there were no previous published literature records of this species as a mycoparasite of powdery mildew disease on sycamore (Platanus occidentalis L.) and crape myrtle (Lagerstroemia indica L.) hosts.Many researches on A. quisqualis has focused on its potential use as a biocontrol agent against powdery mildews on various crops and woody plants [8,9]. At present, a few Ampelomyces isolates have already been registered as biocontrol agents of powdery mildews, e.g. AQ10 biofungicide is commercially used for the biocontrol of grape powdery mildew [10].Recent research on the phylogenetic analysis based on the internal transcribed spacer (ITS) rDNA sequences revealed that the species could be classified into five different genetic clades, showing that different physiological forms exist within genetically diverse strains of the species [11]. P. occidentalis is widely planted as a street tree distributed in South Korea and is useful in the rehabilitation of various sites with saturated soils. Moreover, it has been used for furniture, veneer, and flooring. Crape myrtle (L. indica) is used as a decorative tree. It flowers early in the summer and produces seedpods that contains the alkaloids of interesting biological activity [12].Powdery mildew has frequently been observed on sycamore plants in Jeonnam province, South Korea since it was reported by Lee et al. [13]. The use of biocontrol agents is encouraged for the control of this disease to enhance environmental sust...

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“…There is still an ongoing debate on the taxonomy of the genus Ampelomyces. Although its genetic diversity has been investigated during the last five years and four distinct groups identified according to sequences divergence in the ITS rDNA and actin genes (Park et al, 2010), a single name is still applied to all pycnidial intracellular hyperparasites of powdery mildew (inducing a high possibility of confusion with isolates of Phoma glomerata, according to Sullivan and White (2000)). Recently, it has been reported that the host specificity of Ampelomyces isolates was not so pronounced since some isolates were shown to be able to infect powdery mildew species they were unlikely to have encountered in nature (Kiss et al, 2011).…”

Section: Ampelomycesmentioning

confidence: 99%

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

2011

EFSA Journal

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EFSA is requested to assess the safety of a broad range of biological agents (including microorganisms and viruses) in the context of notifications for market authorisation as sources of food and feed additives, enzymes and plant protection products. The qualified presumption of safety (QPS) assessment was developed by EFSA for its own use to provide a generic risk assessment approach applicable across EFSA's scientific Panels, for biological agents notified for intentional use in the whole food chain. The safety of unambiguously defined biological agents at the highest taxonomic unit that is appropriate for the purpose for which an application is intended and the completeness of the body of knowledge are assessed. Identified safety concerns for a taxonomic unit are where sensible reflected as ‘qualifications’ when a recommendation for the QPS list is given. The list of QPS recommended biological agents is reviewed and updated annually. Therefore, the only valid list is the one in the most recent scientific opinion. The 2011 update reviews microorganisms previously assessed including bacteria, yeasts, filamentous fungi and viruses used for plant protection purposes and confirms the previous recommendations. The anamorph yeast form Phaffia rhodozyma of Xanthophyllomyces dendrorhous was included on the QPS list and to the qualification for yeasts ‘absence of resistance to antimycotics used for medical treatment of yeast infections’, the sentence was added that ‘in the case of Saccharomyces cerevisiae this qualification applies for yeast strains able to grow above 37 °C’. The body of knowledge of filamentous fungi and enterococci was updated and their ineligibility for the QPS list confirmed.

show abstract

Genetic variability and mycohost association of Ampelomyces quisqualis isolates inferred from phylogenetic analyses of ITS rDNA and actin gene sequences

Cited by 35 publications

References 29 publications

Existence of different physiological forms within genetically diverse strains of Ampelomyces quisqualis

Existence of different physiological forms within genetically diverse strains of Ampelomyces quisqualis

First Report of Ampelomyces quisqualis from Sycamore and Crape Myrtle and Its Potential as a Mycoparasite of Powdery Mildew

Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

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